Establishing patient-derived organoids from human endometrial cancer and normal endometrium
Endometrial cancer is the most common gynecologic malignancy in the United States and is one of the few malignancies that had an increasing incidence and mortality rate over the last 10 years. Current research models fail to recapitulate actual characteristics of the tumor that are necessary for the...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1059228/full |
| _version_ | 1797847222187982848 |
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| author | Arielle Katcher Arielle Katcher Brian Yueh Kadir Ozler Aaron Nizam Aaron Nizam Ariel Kredentser Ariel Kredentser Charlie Chung Marina Frimer Marina Frimer Gary L. Goldberg Gary L. Goldberg Gary L. Goldberg Semir Beyaz |
| author_facet | Arielle Katcher Arielle Katcher Brian Yueh Kadir Ozler Aaron Nizam Aaron Nizam Ariel Kredentser Ariel Kredentser Charlie Chung Marina Frimer Marina Frimer Gary L. Goldberg Gary L. Goldberg Gary L. Goldberg Semir Beyaz |
| author_sort | Arielle Katcher |
| collection | DOAJ |
| description | Endometrial cancer is the most common gynecologic malignancy in the United States and is one of the few malignancies that had an increasing incidence and mortality rate over the last 10 years. Current research models fail to recapitulate actual characteristics of the tumor that are necessary for the proper understanding and treatment of this heterogenous disease. Patient-derived organoids provide a durable and versatile culture system that can capture patient-specific characteristics such as the mutational profile and response to therapy of the primary tumor. Here we describe the methods for establishing, expansion and banking of endometrial cancer organoids to develop a living biobank. Samples of both endometrial tumor tissue and matched normal endometrium were collected from 10 patients. The tissue was digested into single cells and then cultured in optimized media to establish matched patient endometrial cancer and normal endometrial tissue organoids. Organoids were created from all major endometrial cancer histologic subtypes. These organoids are passaged long term, banked and can be utilized for downstream histological and genomic characterization as well as functional assays such as assessing the response to therapeutic drugs. |
| first_indexed | 2024-04-09T18:07:50Z |
| format | Article |
| id | doaj.art-57bc388357ca48009ba5ce3e776eacbf |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-09T18:07:50Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-57bc388357ca48009ba5ce3e776eacbf2023-04-14T05:05:56ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-04-011410.3389/fendo.2023.10592281059228Establishing patient-derived organoids from human endometrial cancer and normal endometriumArielle Katcher0Arielle Katcher1Brian Yueh2Kadir Ozler3Aaron Nizam4Aaron Nizam5Ariel Kredentser6Ariel Kredentser7Charlie Chung8Marina Frimer9Marina Frimer10Gary L. Goldberg11Gary L. Goldberg12Gary L. Goldberg13Semir Beyaz14Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Long Island Jewish Medical Center, Zucker School of Medicine at Hofstra/Northwell, Northwell Health, New Hyde Park, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Long Island Jewish Medical Center, Zucker School of Medicine at Hofstra/Northwell, Northwell Health, New Hyde Park, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Long Island Jewish Medical Center, Zucker School of Medicine at Hofstra/Northwell, Northwell Health, New Hyde Park, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Long Island Jewish Medical Center, Zucker School of Medicine at Hofstra/Northwell, Northwell Health, New Hyde Park, NY, United StatesInstitute for Molecular Medicine, Feinstein Institutes for Medical Research, Manhasset, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Long Island Jewish Medical Center, Zucker School of Medicine at Hofstra/Northwell, Northwell Health, New Hyde Park, NY, United StatesInstitute for Molecular Medicine, Feinstein Institutes for Medical Research, Manhasset, NY, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, NY, United StatesEndometrial cancer is the most common gynecologic malignancy in the United States and is one of the few malignancies that had an increasing incidence and mortality rate over the last 10 years. Current research models fail to recapitulate actual characteristics of the tumor that are necessary for the proper understanding and treatment of this heterogenous disease. Patient-derived organoids provide a durable and versatile culture system that can capture patient-specific characteristics such as the mutational profile and response to therapy of the primary tumor. Here we describe the methods for establishing, expansion and banking of endometrial cancer organoids to develop a living biobank. Samples of both endometrial tumor tissue and matched normal endometrium were collected from 10 patients. The tissue was digested into single cells and then cultured in optimized media to establish matched patient endometrial cancer and normal endometrial tissue organoids. Organoids were created from all major endometrial cancer histologic subtypes. These organoids are passaged long term, banked and can be utilized for downstream histological and genomic characterization as well as functional assays such as assessing the response to therapeutic drugs.https://www.frontiersin.org/articles/10.3389/fendo.2023.1059228/fullorganoidendometrial cancerendometriumpatient derivedorganoid culture method |
| spellingShingle | Arielle Katcher Arielle Katcher Brian Yueh Kadir Ozler Aaron Nizam Aaron Nizam Ariel Kredentser Ariel Kredentser Charlie Chung Marina Frimer Marina Frimer Gary L. Goldberg Gary L. Goldberg Gary L. Goldberg Semir Beyaz Establishing patient-derived organoids from human endometrial cancer and normal endometrium Frontiers in Endocrinology organoid endometrial cancer endometrium patient derived organoid culture method |
| title | Establishing patient-derived organoids from human endometrial cancer and normal endometrium |
| title_full | Establishing patient-derived organoids from human endometrial cancer and normal endometrium |
| title_fullStr | Establishing patient-derived organoids from human endometrial cancer and normal endometrium |
| title_full_unstemmed | Establishing patient-derived organoids from human endometrial cancer and normal endometrium |
| title_short | Establishing patient-derived organoids from human endometrial cancer and normal endometrium |
| title_sort | establishing patient derived organoids from human endometrial cancer and normal endometrium |
| topic | organoid endometrial cancer endometrium patient derived organoid culture method |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1059228/full |
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