Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India
Abstract Background Identifying highly immunogenic blood stage antigens which can work as target for naturally acquired antibodies in different eco-epidemiological settings is an important step for designing malaria vaccine. Blood stage proteins of Plasmodium vivax, apical membrane antigen-1 (PvAMA-...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | Malaria Journal |
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| Online Access: | https://doi.org/10.1186/s12936-019-3066-6 |
| _version_ | 1818663973915983872 |
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| author | Sonal Kale Chander P. Yadav Pavitra N. Rao Sneh Shalini Alex Eapen Harish C. Srivasatava Surya K. Sharma Veena Pande Jane M. Carlton Om P. Singh Prashant K. Mallick |
| author_facet | Sonal Kale Chander P. Yadav Pavitra N. Rao Sneh Shalini Alex Eapen Harish C. Srivasatava Surya K. Sharma Veena Pande Jane M. Carlton Om P. Singh Prashant K. Mallick |
| author_sort | Sonal Kale |
| collection | DOAJ |
| description | Abstract Background Identifying highly immunogenic blood stage antigens which can work as target for naturally acquired antibodies in different eco-epidemiological settings is an important step for designing malaria vaccine. Blood stage proteins of Plasmodium vivax, apical membrane antigen-1 (PvAMA-1) and 19 kDa fragment of merozoite surface protein (PvMSP-119) are such promising vaccine candidate antigens. This study determined the naturally-acquired antibody response to PvAMA-1 and PvMSP-119 antigens in individuals living in three geographically diverse malaria endemic regions of India. Methods A total of 234 blood samples were collected from individuals living in three different eco-epidemiological settings, Chennai, Nadiad, and Rourkela of India. Indirect ELISA was performed to measure human IgG antibodies against recombinant PvAMA-1 and PvMSP-119 antigens. The difference in seroprevalence and factors associated with antibody responses at each site was statistically analysed. Results The overall seroprevalence was 40.6% for PvAMA-1 and 62.4% for PvMSP-119. Seroprevalence to PvAMA-1 was higher in Chennai (47%) followed by Nadiad (46.7%) and Rourkela (27.6%). For PvMSP-119, seroprevalence was higher in Chennai (80.3%) as compared to Nadiad (53.3%) and Rourkela (57.9%). Seroprevalence for both the antigens were found to be higher in Chennai where P. vivax is the dominant malaria species. In addition, heterogeneous antibody response was observed for PvAMA-1 and PvMSP-119 antigens at each of the study sites. Two factors, age and malaria positivity were significantly associated with seropositivity for both the antigens PvAMA-1 and PvMSP-119. Conclusion These data suggest that natural acquired antibody response is higher for PvMSP-119 antigen as compared to PvAMA-1 antigen in individuals living in three geographically diverse malaria endemic regions in India. PvMSP-119 appears to be highly immunogenic in Indian population and has great potential as a malaria vaccine candidate. The differences in immune response against vaccine candidate antigens in different endemic settings should be taken into account for development of asexual stage based P. vivax malaria vaccine, which in turn can enhance malaria control efforts. |
| first_indexed | 2024-12-17T05:25:22Z |
| format | Article |
| id | doaj.art-57bc6dad5e394c60af7e8c07f4af14b9 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-17T05:25:22Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-57bc6dad5e394c60af7e8c07f4af14b92022-12-21T22:01:53ZengBMCMalaria Journal1475-28752019-12-0118111310.1186/s12936-019-3066-6Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of IndiaSonal Kale0Chander P. Yadav1Pavitra N. Rao2Sneh Shalini3Alex Eapen4Harish C. Srivasatava5Surya K. Sharma6Veena Pande7Jane M. Carlton8Om P. Singh9Prashant K. Mallick10ICMR-National Institute of Malaria ResearchICMR-National Institute of Malaria ResearchDepartment of Biology, Center for Genomics and Systems Biology, New York UniversityICMR-National Institute of Malaria ResearchNational Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology CampusNational Institute of Malaria Research Field Unit, Civil HospitalJigyansha, International Center of Excellence for Malaria ResearchDepartment of Biotechnology, Kumaun UniversityDepartment of Biology, Center for Genomics and Systems Biology, New York UniversityICMR-National Institute of Malaria ResearchICMR-National Institute of Malaria ResearchAbstract Background Identifying highly immunogenic blood stage antigens which can work as target for naturally acquired antibodies in different eco-epidemiological settings is an important step for designing malaria vaccine. Blood stage proteins of Plasmodium vivax, apical membrane antigen-1 (PvAMA-1) and 19 kDa fragment of merozoite surface protein (PvMSP-119) are such promising vaccine candidate antigens. This study determined the naturally-acquired antibody response to PvAMA-1 and PvMSP-119 antigens in individuals living in three geographically diverse malaria endemic regions of India. Methods A total of 234 blood samples were collected from individuals living in three different eco-epidemiological settings, Chennai, Nadiad, and Rourkela of India. Indirect ELISA was performed to measure human IgG antibodies against recombinant PvAMA-1 and PvMSP-119 antigens. The difference in seroprevalence and factors associated with antibody responses at each site was statistically analysed. Results The overall seroprevalence was 40.6% for PvAMA-1 and 62.4% for PvMSP-119. Seroprevalence to PvAMA-1 was higher in Chennai (47%) followed by Nadiad (46.7%) and Rourkela (27.6%). For PvMSP-119, seroprevalence was higher in Chennai (80.3%) as compared to Nadiad (53.3%) and Rourkela (57.9%). Seroprevalence for both the antigens were found to be higher in Chennai where P. vivax is the dominant malaria species. In addition, heterogeneous antibody response was observed for PvAMA-1 and PvMSP-119 antigens at each of the study sites. Two factors, age and malaria positivity were significantly associated with seropositivity for both the antigens PvAMA-1 and PvMSP-119. Conclusion These data suggest that natural acquired antibody response is higher for PvMSP-119 antigen as compared to PvAMA-1 antigen in individuals living in three geographically diverse malaria endemic regions in India. PvMSP-119 appears to be highly immunogenic in Indian population and has great potential as a malaria vaccine candidate. The differences in immune response against vaccine candidate antigens in different endemic settings should be taken into account for development of asexual stage based P. vivax malaria vaccine, which in turn can enhance malaria control efforts.https://doi.org/10.1186/s12936-019-3066-6Plasmodium vivaxPvAMA-1PvMSP-119Antibody responseSeroprevalenceIndia |
| spellingShingle | Sonal Kale Chander P. Yadav Pavitra N. Rao Sneh Shalini Alex Eapen Harish C. Srivasatava Surya K. Sharma Veena Pande Jane M. Carlton Om P. Singh Prashant K. Mallick Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India Malaria Journal Plasmodium vivax PvAMA-1 PvMSP-119 Antibody response Seroprevalence India |
| title | Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India |
| title_full | Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India |
| title_fullStr | Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India |
| title_full_unstemmed | Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India |
| title_short | Antibody responses within two leading Plasmodium vivax vaccine candidate antigens in three geographically diverse malaria-endemic regions of India |
| title_sort | antibody responses within two leading plasmodium vivax vaccine candidate antigens in three geographically diverse malaria endemic regions of india |
| topic | Plasmodium vivax PvAMA-1 PvMSP-119 Antibody response Seroprevalence India |
| url | https://doi.org/10.1186/s12936-019-3066-6 |
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