Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects
Cellular magnetic field effects are assumed to base on coherent singlet-triplet interconversion of radical pairs that are sensitive to applied radiofrequency (RF) and weak magnetic fields (WEMFs), known as radical pair mechanism (RPM). As a leading model, the RPM explains how quantum effects can inf...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Redox Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231724001289 |
| _version_ | 1797215953065345024 |
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| author | Viktoria Thoeni Elitsa Y. Dimova Thomas Kietzmann Robert J. Usselman Margit Egg |
| author_facet | Viktoria Thoeni Elitsa Y. Dimova Thomas Kietzmann Robert J. Usselman Margit Egg |
| author_sort | Viktoria Thoeni |
| collection | DOAJ |
| description | Cellular magnetic field effects are assumed to base on coherent singlet-triplet interconversion of radical pairs that are sensitive to applied radiofrequency (RF) and weak magnetic fields (WEMFs), known as radical pair mechanism (RPM). As a leading model, the RPM explains how quantum effects can influence biochemical and cellular signalling. Consequently, radical pairs generate reactive oxygen species (ROS) that link the RPM to redox processes, such as the response to hypoxia and the circadian clock. Therapeutic nuclear magnetic resonance (tNMR) occupies a unique position in the RPM paradigm because of the used frequencies, which are far below the range of 0.1–100 MHz postulated for the RPM to occur. Nonetheless, tNMR was shown to induce RPM like effects, such as increased extracellular H2O2 levels and altered cellular bioenergetics. In this study we compared the impact of tNMR and intermittent hypoxia on the circadian clock, as well as the role of superoxide in tNMR induced ROS partitioning. We show that both, tNMR and intermittent hypoxia, exert on/off effects on cellular clocks that are dependent on the time of application (day versus night). In addition, our data provide further evidence that superoxide plays a central role in magnetic signal transduction. tNMR used in combination with scavengers, such as Vitamin C, led to strong ROS product redistributions. This discovery might represent the first indication of radical triads in biological systems. |
| first_indexed | 2024-04-24T11:38:15Z |
| format | Article |
| id | doaj.art-57bf73ef6b5d4d4eb3c3e49ac1d21fc0 |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-04-24T11:38:15Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-57bf73ef6b5d4d4eb3c3e49ac1d21fc02024-04-10T04:28:57ZengElsevierRedox Biology2213-23172024-06-0172103152Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effectsViktoria Thoeni0Elitsa Y. Dimova1Thomas Kietzmann2Robert J. Usselman3Margit Egg4Institute of Zoology, University Innsbruck, Technikerstraße 25, 6020, Innsbruck, Tyrol, A-6020, AustriaFaculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, P.O. Box 3000, 90014, Oulu, FinlandFaculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, P.O. Box 3000, 90014, Oulu, FinlandFaculty of Chemistry and Chemical Engineering, Florida Institute of Technology, 150 W University Blvd, Melbourne, FL, 32901, USAInstitute of Zoology, University Innsbruck, Technikerstraße 25, 6020, Innsbruck, Tyrol, A-6020, Austria; Corresponding author. Institute of Zoology, University of Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria.Cellular magnetic field effects are assumed to base on coherent singlet-triplet interconversion of radical pairs that are sensitive to applied radiofrequency (RF) and weak magnetic fields (WEMFs), known as radical pair mechanism (RPM). As a leading model, the RPM explains how quantum effects can influence biochemical and cellular signalling. Consequently, radical pairs generate reactive oxygen species (ROS) that link the RPM to redox processes, such as the response to hypoxia and the circadian clock. Therapeutic nuclear magnetic resonance (tNMR) occupies a unique position in the RPM paradigm because of the used frequencies, which are far below the range of 0.1–100 MHz postulated for the RPM to occur. Nonetheless, tNMR was shown to induce RPM like effects, such as increased extracellular H2O2 levels and altered cellular bioenergetics. In this study we compared the impact of tNMR and intermittent hypoxia on the circadian clock, as well as the role of superoxide in tNMR induced ROS partitioning. We show that both, tNMR and intermittent hypoxia, exert on/off effects on cellular clocks that are dependent on the time of application (day versus night). In addition, our data provide further evidence that superoxide plays a central role in magnetic signal transduction. tNMR used in combination with scavengers, such as Vitamin C, led to strong ROS product redistributions. This discovery might represent the first indication of radical triads in biological systems.http://www.sciencedirect.com/science/article/pii/S2213231724001289Circadian clockMagnetic field effectsIntermittent hypoxiaReactive oxygen speciesSuperoxide |
| spellingShingle | Viktoria Thoeni Elitsa Y. Dimova Thomas Kietzmann Robert J. Usselman Margit Egg Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects Redox Biology Circadian clock Magnetic field effects Intermittent hypoxia Reactive oxygen species Superoxide |
| title | Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects |
| title_full | Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects |
| title_fullStr | Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects |
| title_full_unstemmed | Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects |
| title_short | Therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on/off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects |
| title_sort | therapeutic nuclear magnetic resonance and intermittent hypoxia trigger time dependent on off effects in circadian clocks and confirm a central role of superoxide in cellular magnetic field effects |
| topic | Circadian clock Magnetic field effects Intermittent hypoxia Reactive oxygen species Superoxide |
| url | http://www.sciencedirect.com/science/article/pii/S2213231724001289 |
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