Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene
Dicyclopropanated 5-vinyl-2-norbornene (dcpVNB) is a strained polycyclic hydrocarbon compound with a high energy content, which makes it promising for the development of propellant components based on it. In this work, the genotoxic properties of dcpVNB were studied using whole-cell <i>lux<...
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/1/57 |
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| author | Uliana S. Novoyatlova Andrei G. Kessenikh Olga V. Kononchuk Sergey V. Bazhenov Alexander A. Fomkin Anna A. Kudryavtseva Sergey V. Shorunov Maxim V. Bermeshev Ilya V. Manukhov |
| author_facet | Uliana S. Novoyatlova Andrei G. Kessenikh Olga V. Kononchuk Sergey V. Bazhenov Alexander A. Fomkin Anna A. Kudryavtseva Sergey V. Shorunov Maxim V. Bermeshev Ilya V. Manukhov |
| author_sort | Uliana S. Novoyatlova |
| collection | DOAJ |
| description | Dicyclopropanated 5-vinyl-2-norbornene (dcpVNB) is a strained polycyclic hydrocarbon compound with a high energy content, which makes it promising for the development of propellant components based on it. In this work, the genotoxic properties of dcpVNB were studied using whole-cell <i>lux</i>-biosensors based on <i>Escherichia coli</i> and <i>Bacillus subtilis</i>. It was shown that the addition of dcpVNB to bacterial cells leads to the appearance of DNA damage inducing the SOS response and Dps expression with slight activation of the OxyR-mediated response to oxidative stress. The highest toxic effect of dcpVNB is detected by the following <i>lux</i>-biosensors: <i>E. coli</i> pColD-lux, <i>E. coli</i> pDps, <i>B. subtilis</i> pNK-DinC, and <i>B. subtilis</i> pNK-MrgA, in which the genes of bacterial luciferases are transcriptionally fused to the corresponding promoters: P<i><sub>cda</sub></i>, P<i><sub>dps</sub></i>, P<i><sub>dinC</sub></i>, and P<i><sub>mrgA</sub></i>. It was shown that <i>lux</i>-biosensors based on <i>B. subtilis,</i> and <i>E. coli</i> are almost equally sensitive to dcpVNB, which indicates the same permeability to this compound of cell wall of Gram-positive and Gram-negative bacteria. The activation of P<i><sub>dps</sub></i> after dcpVNB addition maintains even in <i>oxyR</i> mutant <i>E. coli</i> strains, which means that the P<i><sub>dps</sub></i> induction is only partially determined by the OxyR/S regulon. Comparison of specific stress effects caused by dcpVNB and 2-ethyl(bicyclo[2.2.1]heptane) (EBH), characterized by the absence of cyclopropanated groups, shows that structural changes in hydrocarbons could significantly change the mode of toxicity. |
| first_indexed | 2024-03-09T13:25:16Z |
| format | Article |
| id | doaj.art-57bfc0d1c6f34d81b46d4dc39dac8532 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T13:25:16Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-57bfc0d1c6f34d81b46d4dc39dac85322023-11-30T21:24:52ZengMDPI AGBiosensors2079-63742022-12-011315710.3390/bios13010057Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-NorborneneUliana S. Novoyatlova0Andrei G. Kessenikh1Olga V. Kononchuk2Sergey V. Bazhenov3Alexander A. Fomkin4Anna A. Kudryavtseva5Sergey V. Shorunov6Maxim V. Bermeshev7Ilya V. Manukhov8Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaResearch Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaResearch Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaResearch Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaResearch Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaResearch Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaTopchiev Institute of Petrochemical Synthesis, RAS, 119071 Moscow, RussiaTopchiev Institute of Petrochemical Synthesis, RAS, 119071 Moscow, RussiaResearch Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaDicyclopropanated 5-vinyl-2-norbornene (dcpVNB) is a strained polycyclic hydrocarbon compound with a high energy content, which makes it promising for the development of propellant components based on it. In this work, the genotoxic properties of dcpVNB were studied using whole-cell <i>lux</i>-biosensors based on <i>Escherichia coli</i> and <i>Bacillus subtilis</i>. It was shown that the addition of dcpVNB to bacterial cells leads to the appearance of DNA damage inducing the SOS response and Dps expression with slight activation of the OxyR-mediated response to oxidative stress. The highest toxic effect of dcpVNB is detected by the following <i>lux</i>-biosensors: <i>E. coli</i> pColD-lux, <i>E. coli</i> pDps, <i>B. subtilis</i> pNK-DinC, and <i>B. subtilis</i> pNK-MrgA, in which the genes of bacterial luciferases are transcriptionally fused to the corresponding promoters: P<i><sub>cda</sub></i>, P<i><sub>dps</sub></i>, P<i><sub>dinC</sub></i>, and P<i><sub>mrgA</sub></i>. It was shown that <i>lux</i>-biosensors based on <i>B. subtilis,</i> and <i>E. coli</i> are almost equally sensitive to dcpVNB, which indicates the same permeability to this compound of cell wall of Gram-positive and Gram-negative bacteria. The activation of P<i><sub>dps</sub></i> after dcpVNB addition maintains even in <i>oxyR</i> mutant <i>E. coli</i> strains, which means that the P<i><sub>dps</sub></i> induction is only partially determined by the OxyR/S regulon. Comparison of specific stress effects caused by dcpVNB and 2-ethyl(bicyclo[2.2.1]heptane) (EBH), characterized by the absence of cyclopropanated groups, shows that structural changes in hydrocarbons could significantly change the mode of toxicity.https://www.mdpi.com/2079-6374/13/1/57<i>lux-</i>biosensorgenotoxicitystrained hydrocarbonsfuel |
| spellingShingle | Uliana S. Novoyatlova Andrei G. Kessenikh Olga V. Kononchuk Sergey V. Bazhenov Alexander A. Fomkin Anna A. Kudryavtseva Sergey V. Shorunov Maxim V. Bermeshev Ilya V. Manukhov Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene Biosensors <i>lux-</i>biosensor genotoxicity strained hydrocarbons fuel |
| title | Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene |
| title_full | Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene |
| title_fullStr | Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene |
| title_full_unstemmed | Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene |
| title_short | Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene |
| title_sort | genotoxic effect of dicyclopropanated 5 vinyl 2 norbornene |
| topic | <i>lux-</i>biosensor genotoxicity strained hydrocarbons fuel |
| url | https://www.mdpi.com/2079-6374/13/1/57 |
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