Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I
Abstract Feline calicivirus (FCV) and feline herpesvirus type I (FHV-1) are the most common viral pathogens responsible for cat respiratory diseases, and coinfection with these two pathogens is often found. In veterinary clinics, the main diagnostic methods for FCV and FHV-1 are test strips and poly...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02020-3 |
| _version_ | 1797850227530530816 |
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| author | Bo Chen Haoyang Zhang Hanhong Wang Shoujun Li Pei Zhou |
| author_facet | Bo Chen Haoyang Zhang Hanhong Wang Shoujun Li Pei Zhou |
| author_sort | Bo Chen |
| collection | DOAJ |
| description | Abstract Feline calicivirus (FCV) and feline herpesvirus type I (FHV-1) are the most common viral pathogens responsible for cat respiratory diseases, and coinfection with these two pathogens is often found. In veterinary clinics, the main diagnostic methods for FCV and FHV-1 are test strips and polymerase chain reaction (PCR). However, the sensitivity of test strips are not sufficient, and PCR is time-consuming. Therefore, developing a rapid and high-performance clinical diagnostic test is imperative for the prevention and treatment of these diseases. Enzymatic recombinase amplification (ERA) is an automated isothermal nucleic acid amplification technique that maintains a constant temperature, and is both rapid and highly accurate. In this study, a dual ERA method was developed using the Exo probe for a differential detection of FCV and FHV-1. This dual ERA method demonstrated high performance with the detection limit of 101 copies for both viruses, and no cross-reactions with feline parvovirus virus and F81 cells. To test the utility of the method for clinical applications, 50 nasopharyngeal swabs from cats with respiratory symptoms were collected and tested. The positive rates of FCV and FHV-1 were 40% (20/50, 95% confidence interval [CI], 26.4 to 54.8%) and 14% (7/50, 95% CI, 5.8 to 26.7%), respectively. The rate of coinfection with FCV and FHV-1 was 10% (5/50, 95% CI, 3.3 to 21.8%). These results were in agreement with those found using quantitative real-time PCR. Therefore, this dual ERA method is a novel and efficient clinical diagnostic tool for FCV and FHV-1 detection. |
| first_indexed | 2024-04-09T18:57:01Z |
| format | Article |
| id | doaj.art-57c1d9220a894ea3ab48e55723495fb1 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T18:57:01Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-57c1d9220a894ea3ab48e55723495fb12023-04-09T11:07:57ZengBMCVirology Journal1743-422X2023-04-012011810.1186/s12985-023-02020-3Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type IBo Chen0Haoyang Zhang1Hanhong Wang2Shoujun Li3Pei Zhou4Guangdong Provincial Pet Engineering Technology Research Center, College of Veterinary Medicine, South China Agricultural UniversityGuangdong Provincial Pet Engineering Technology Research Center, College of Veterinary Medicine, South China Agricultural UniversityGuangdong Provincial Pet Engineering Technology Research Center, College of Veterinary Medicine, South China Agricultural UniversityGuangdong Provincial Pet Engineering Technology Research Center, College of Veterinary Medicine, South China Agricultural UniversityGuangdong Provincial Pet Engineering Technology Research Center, College of Veterinary Medicine, South China Agricultural UniversityAbstract Feline calicivirus (FCV) and feline herpesvirus type I (FHV-1) are the most common viral pathogens responsible for cat respiratory diseases, and coinfection with these two pathogens is often found. In veterinary clinics, the main diagnostic methods for FCV and FHV-1 are test strips and polymerase chain reaction (PCR). However, the sensitivity of test strips are not sufficient, and PCR is time-consuming. Therefore, developing a rapid and high-performance clinical diagnostic test is imperative for the prevention and treatment of these diseases. Enzymatic recombinase amplification (ERA) is an automated isothermal nucleic acid amplification technique that maintains a constant temperature, and is both rapid and highly accurate. In this study, a dual ERA method was developed using the Exo probe for a differential detection of FCV and FHV-1. This dual ERA method demonstrated high performance with the detection limit of 101 copies for both viruses, and no cross-reactions with feline parvovirus virus and F81 cells. To test the utility of the method for clinical applications, 50 nasopharyngeal swabs from cats with respiratory symptoms were collected and tested. The positive rates of FCV and FHV-1 were 40% (20/50, 95% confidence interval [CI], 26.4 to 54.8%) and 14% (7/50, 95% CI, 5.8 to 26.7%), respectively. The rate of coinfection with FCV and FHV-1 was 10% (5/50, 95% CI, 3.3 to 21.8%). These results were in agreement with those found using quantitative real-time PCR. Therefore, this dual ERA method is a novel and efficient clinical diagnostic tool for FCV and FHV-1 detection.https://doi.org/10.1186/s12985-023-02020-3ERAFCVFHV-1Dual detectionClinical detection |
| spellingShingle | Bo Chen Haoyang Zhang Hanhong Wang Shoujun Li Pei Zhou Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I Virology Journal ERA FCV FHV-1 Dual detection Clinical detection |
| title | Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I |
| title_full | Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I |
| title_fullStr | Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I |
| title_full_unstemmed | Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I |
| title_short | Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I |
| title_sort | development and application of a dual era method for the detection of feline calicivirus and feline herpesvirus type i |
| topic | ERA FCV FHV-1 Dual detection Clinical detection |
| url | https://doi.org/10.1186/s12985-023-02020-3 |
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