Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
Abstract We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to...
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Nature Portfolio
2017-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-15582-4 |
| _version_ | 1818844068168335360 |
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| author | J. Peters J. Marion F. J. Becher M. Trapp T. Gutberlet D. J. Bicout T. Heimburg |
| author_facet | J. Peters J. Marion F. J. Becher M. Trapp T. Gutberlet D. J. Bicout T. Heimburg |
| author_sort | J. Peters |
| collection | DOAJ |
| description | Abstract We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to relevant thermodynamic quantities, and elastic incoherent neutron scattering (EINS) that probes local motions of a system at the atomic level by allowing extraction of dynamical parameters. PPC revealed that the volume expansion coefficient of DMPC and DMPC/Cholesterol samples with 13 and 25 mol% cholesterol is a linear function of the heat capacity measured by differential scanning calorimetry. Neutron backscattering spectroscopy showed that the mean square displacements of H atoms do exhibit an increase with temperature and a decrease under increasing pressure. Cholesterol added at concentrations of 25 and 50 mol% led to suppression of the main phase transition. Taking advantage of these results, the present study aims (i) to show that calorimetry and EINS using the Bicout and Zaccai model equally permit to get access to thermodynamic quantities characterizing pure DMPC and DMPC/cholesterol mixtures, thus directly confirming the theoretical method, and (ii) to validate our approach as function of temperature and of pressure, as both are equally important and complementary thermodynamic variables. |
| first_indexed | 2024-12-19T05:07:53Z |
| format | Article |
| id | doaj.art-57c3499640cc4f67867d4c378b8b2703 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:07:53Z |
| publishDate | 2017-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-57c3499640cc4f67867d4c378b8b27032022-12-21T20:34:53ZengNature PortfolioScientific Reports2045-23222017-11-017111510.1038/s41598-017-15582-4Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressureJ. Peters0J. Marion1F. J. Becher2M. Trapp3T. Gutberlet4D. J. Bicout5T. Heimburg6Univ. Grenoble Alpes, LiPhy, 140 Rue de la PhysiqueUniv. Grenoble Alpes, LiPhy, 140 Rue de la PhysiqueNiels Bohr Institute, University of Copenhagen, Blegdamsvej 17Helmholtz-Zentrum Berlin für Materialien und Energie, Lise-Meitner Campus, Hahn-Meitner-Platz 1Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Lichtenbergstr. 1Institut Laue-Langevin, 71 avenue des Martyrs, CS 20156Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17Abstract We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to relevant thermodynamic quantities, and elastic incoherent neutron scattering (EINS) that probes local motions of a system at the atomic level by allowing extraction of dynamical parameters. PPC revealed that the volume expansion coefficient of DMPC and DMPC/Cholesterol samples with 13 and 25 mol% cholesterol is a linear function of the heat capacity measured by differential scanning calorimetry. Neutron backscattering spectroscopy showed that the mean square displacements of H atoms do exhibit an increase with temperature and a decrease under increasing pressure. Cholesterol added at concentrations of 25 and 50 mol% led to suppression of the main phase transition. Taking advantage of these results, the present study aims (i) to show that calorimetry and EINS using the Bicout and Zaccai model equally permit to get access to thermodynamic quantities characterizing pure DMPC and DMPC/cholesterol mixtures, thus directly confirming the theoretical method, and (ii) to validate our approach as function of temperature and of pressure, as both are equally important and complementary thermodynamic variables.https://doi.org/10.1038/s41598-017-15582-4 |
| spellingShingle | J. Peters J. Marion F. J. Becher M. Trapp T. Gutberlet D. J. Bicout T. Heimburg Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure Scientific Reports |
| title | Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure |
| title_full | Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure |
| title_fullStr | Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure |
| title_full_unstemmed | Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure |
| title_short | Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure |
| title_sort | thermodynamics of lipid multi lamellar vesicles in presence of sterols at high hydrostatic pressure |
| url | https://doi.org/10.1038/s41598-017-15582-4 |
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