The core exosome proteome of Trichomonas vaginalis
Background: Trichomonas vaginalis is parasitic protozoan that causes human urogenital infections. Accumulated reports indicated that exosomes released by this parasite play a crucial role in transmitting information and substances between cells during host-parasite interactions. Current knowledge on...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118224000409 |
| _version_ | 1797248341000585216 |
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| author | Seow-Chin Ong Hong-Wei Luo Wei-Hung Cheng Fu-Man Ku Chih-Yu Tsai Po-Jung Huang Chi-Ching Lee Yuan-Ming Yeh Rose Lin Cheng-Hsun Chiu Petrus Tang |
| author_facet | Seow-Chin Ong Hong-Wei Luo Wei-Hung Cheng Fu-Man Ku Chih-Yu Tsai Po-Jung Huang Chi-Ching Lee Yuan-Ming Yeh Rose Lin Cheng-Hsun Chiu Petrus Tang |
| author_sort | Seow-Chin Ong |
| collection | DOAJ |
| description | Background: Trichomonas vaginalis is parasitic protozoan that causes human urogenital infections. Accumulated reports indicated that exosomes released by this parasite play a crucial role in transmitting information and substances between cells during host-parasite interactions. Current knowledge on the protein contents in T. vaginalis exosome is mainly generated from three previous studies that used different T. vaginalis isolates as an experimental model. Whether T. vaginalis exosomes comprise a common set of proteins (core exosome proteome) is still unclear. Methods: To explore the core exosome proteome in T. vaginalis, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the contents of sucrose ultracentrifugation-enriched exosome and supernatant fractions isolated from six isolates. Results: Transmission electron microscopy (TEM) confirmed the presence of exosomes in the enriched fraction. Proteomic analysis identified a total of 1870 proteins from exosomal extracts. There were 1207 exosomal-specific proteins after excluding 436 ‘non-core exosomal proteins’. Among these, 72 common exosomal-specific proteins were expressed in all six isolates. Compared with three published T. vaginalis exosome proteome datasets, we identified 16 core exosomal-specific proteins. These core exosomal-specific proteins included tetraspanin (TvTSP1), the classical exosome marker, and proteins mainly involved in catalytic activity and binding such as ribosomal proteins, ras-associated binding (Rab) proteins, and heterotrimeric G proteins. Conclusions: Our study highlighted the importance of using supernatant fraction from exosomal extract as a control to eliminate ‘non-core exosomal proteins’. We compiled a reference core exosome proteome of T. vaginalis, which is essential for developing a fundamental understanding of exosome-mediated cell communication and host-parasite interaction. |
| first_indexed | 2024-04-24T20:13:02Z |
| format | Article |
| id | doaj.art-57c3c8a17cbc49d3a72d8710a05389f3 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-04-24T20:13:02Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-57c3c8a17cbc49d3a72d8710a05389f32024-03-23T06:23:31ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822024-04-01572246256The core exosome proteome of Trichomonas vaginalisSeow-Chin Ong0Hong-Wei Luo1Wei-Hung Cheng2Fu-Man Ku3Chih-Yu Tsai4Po-Jung Huang5Chi-Ching Lee6Yuan-Ming Yeh7Rose Lin8Cheng-Hsun Chiu9Petrus Tang10Department of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, TaiwanDepartment of Parasitology, College of Medicine, National Cheng Kung University, Tainan City, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, TaiwanDepartment of Biomedical Sciences, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan; Genomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, TaiwanGenomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Guishan District, Taoyuan City, TaiwanGenomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, TaiwanMolecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Linkou, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Guishan District, Taoyuan City, Taiwan; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Linkou, Taiwan; Corresponding author. Department of Parasitology, College of Medicine, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan.Background: Trichomonas vaginalis is parasitic protozoan that causes human urogenital infections. Accumulated reports indicated that exosomes released by this parasite play a crucial role in transmitting information and substances between cells during host-parasite interactions. Current knowledge on the protein contents in T. vaginalis exosome is mainly generated from three previous studies that used different T. vaginalis isolates as an experimental model. Whether T. vaginalis exosomes comprise a common set of proteins (core exosome proteome) is still unclear. Methods: To explore the core exosome proteome in T. vaginalis, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the contents of sucrose ultracentrifugation-enriched exosome and supernatant fractions isolated from six isolates. Results: Transmission electron microscopy (TEM) confirmed the presence of exosomes in the enriched fraction. Proteomic analysis identified a total of 1870 proteins from exosomal extracts. There were 1207 exosomal-specific proteins after excluding 436 ‘non-core exosomal proteins’. Among these, 72 common exosomal-specific proteins were expressed in all six isolates. Compared with three published T. vaginalis exosome proteome datasets, we identified 16 core exosomal-specific proteins. These core exosomal-specific proteins included tetraspanin (TvTSP1), the classical exosome marker, and proteins mainly involved in catalytic activity and binding such as ribosomal proteins, ras-associated binding (Rab) proteins, and heterotrimeric G proteins. Conclusions: Our study highlighted the importance of using supernatant fraction from exosomal extract as a control to eliminate ‘non-core exosomal proteins’. We compiled a reference core exosome proteome of T. vaginalis, which is essential for developing a fundamental understanding of exosome-mediated cell communication and host-parasite interaction.http://www.sciencedirect.com/science/article/pii/S1684118224000409Core proteomeExosomeExtracellular vesiclesTrichomonas vaginalis |
| spellingShingle | Seow-Chin Ong Hong-Wei Luo Wei-Hung Cheng Fu-Man Ku Chih-Yu Tsai Po-Jung Huang Chi-Ching Lee Yuan-Ming Yeh Rose Lin Cheng-Hsun Chiu Petrus Tang The core exosome proteome of Trichomonas vaginalis Journal of Microbiology, Immunology and Infection Core proteome Exosome Extracellular vesicles Trichomonas vaginalis |
| title | The core exosome proteome of Trichomonas vaginalis |
| title_full | The core exosome proteome of Trichomonas vaginalis |
| title_fullStr | The core exosome proteome of Trichomonas vaginalis |
| title_full_unstemmed | The core exosome proteome of Trichomonas vaginalis |
| title_short | The core exosome proteome of Trichomonas vaginalis |
| title_sort | core exosome proteome of trichomonas vaginalis |
| topic | Core proteome Exosome Extracellular vesicles Trichomonas vaginalis |
| url | http://www.sciencedirect.com/science/article/pii/S1684118224000409 |
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