Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells
Prostaglandins are a group of lipids that produce diverse physiological and pathological effects. Among them, prostaglandin E2 (PGE2) stands out for the wide variety of functions in which it participates. To date, there is little information about the influence of PGE2 on gap junctional intercellula...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/11/5813 |
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| author | Alejandro Ogazon del Toro Lidia Jimenez Mauricio Serrano Rubi Aida Castillo Lorena Hinojosa Jacqueline Martinez Rendon Marcelino Cereijido Arturo Ponce |
| author_facet | Alejandro Ogazon del Toro Lidia Jimenez Mauricio Serrano Rubi Aida Castillo Lorena Hinojosa Jacqueline Martinez Rendon Marcelino Cereijido Arturo Ponce |
| author_sort | Alejandro Ogazon del Toro |
| collection | DOAJ |
| description | Prostaglandins are a group of lipids that produce diverse physiological and pathological effects. Among them, prostaglandin E2 (PGE2) stands out for the wide variety of functions in which it participates. To date, there is little information about the influence of PGE2 on gap junctional intercellular communication (GJIC) in any type of tissue, including epithelia. In this work, we set out to determine whether PGE2 influences GJIC in epithelial cells (MDCK cells). To this end, we performed dye (Lucifer yellow) transfer assays to compare GJIC of MDCK cells treated with PGE2 and untreated cells. Our results indicated that (1) PGE2 induces a statistically significant increase in GJIC from 100 nM and from 15 min after its addition to the medium, (2) such effect does not require the synthesis of new mRNA or proteins subunits but rather trafficking of subunits already synthesized, and (3) such effect is mediated by the E2 receptor, which, in turn, triggers a signaling pathway that includes activation of adenylyl cyclase and protein kinase A (PKA). These results widen the knowledge regarding modulation of gap junctional intercellular communication by prostaglandins. |
| first_indexed | 2024-03-10T10:55:31Z |
| format | Article |
| id | doaj.art-57c3f3d7a5c747248b87b999e035de2d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:55:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-57c3f3d7a5c747248b87b999e035de2d2023-11-21T21:55:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211581310.3390/ijms22115813Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial CellsAlejandro Ogazon del Toro0Lidia Jimenez1Mauricio Serrano Rubi2Aida Castillo3Lorena Hinojosa4Jacqueline Martinez Rendon5Marcelino Cereijido6Arturo Ponce7Department of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, CDMX, México C.P. 07360, MexicoProstaglandins are a group of lipids that produce diverse physiological and pathological effects. Among them, prostaglandin E2 (PGE2) stands out for the wide variety of functions in which it participates. To date, there is little information about the influence of PGE2 on gap junctional intercellular communication (GJIC) in any type of tissue, including epithelia. In this work, we set out to determine whether PGE2 influences GJIC in epithelial cells (MDCK cells). To this end, we performed dye (Lucifer yellow) transfer assays to compare GJIC of MDCK cells treated with PGE2 and untreated cells. Our results indicated that (1) PGE2 induces a statistically significant increase in GJIC from 100 nM and from 15 min after its addition to the medium, (2) such effect does not require the synthesis of new mRNA or proteins subunits but rather trafficking of subunits already synthesized, and (3) such effect is mediated by the E2 receptor, which, in turn, triggers a signaling pathway that includes activation of adenylyl cyclase and protein kinase A (PKA). These results widen the knowledge regarding modulation of gap junctional intercellular communication by prostaglandins.https://www.mdpi.com/1422-0067/22/11/5813prostaglandin E2gap junctiondye transferepithelia |
| spellingShingle | Alejandro Ogazon del Toro Lidia Jimenez Mauricio Serrano Rubi Aida Castillo Lorena Hinojosa Jacqueline Martinez Rendon Marcelino Cereijido Arturo Ponce Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells International Journal of Molecular Sciences prostaglandin E2 gap junction dye transfer epithelia |
| title | Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells |
| title_full | Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells |
| title_fullStr | Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells |
| title_full_unstemmed | Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells |
| title_short | Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells |
| title_sort | prostaglandin e2 enhances gap junctional intercellular communication in clonal epithelial cells |
| topic | prostaglandin E2 gap junction dye transfer epithelia |
| url | https://www.mdpi.com/1422-0067/22/11/5813 |
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