Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota
Ottonia martiana Miq. (Piperaceae), a plant known popularly in southern Brazil as “anestésia” and used in the treatment of odontalgia for its anesthetic action on the oral mucosa, was investigated for antibacterial activity by paper disc agar diffusion and bioautographic methods, against microo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
São Paulo State University (UNESP)
2012-12-01
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| Series: | Revista de Ciências Farmacêuticas Básica e Aplicada |
| Subjects: | |
| Online Access: | http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2398/1325 |
| _version_ | 1818877885438492672 |
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| author | Miriam Machado Cunico Celso Garcia Auer Cristina Peitz de Lima Lilian Cristina Côcco Carlos Itsuo Yamamoto Marilis Dallarmi Miguel Obdulio Gomes Miguel Carlos Roberto Sanquetta |
| author_facet | Miriam Machado Cunico Celso Garcia Auer Cristina Peitz de Lima Lilian Cristina Côcco Carlos Itsuo Yamamoto Marilis Dallarmi Miguel Obdulio Gomes Miguel Carlos Roberto Sanquetta |
| author_sort | Miriam Machado Cunico |
| collection | DOAJ |
| description | Ottonia martiana Miq. (Piperaceae), a plant known
popularly in southern Brazil as “anestésia” and used
in the treatment of odontalgia for its anesthetic action
on the oral mucosa, was investigated for antibacterial
activity by paper disc agar diffusion and bioautographic
methods, against microorganisms present in the human
oral cavity [Streptococcus mutans (ATCC 25175),
Streptococcus mitis (ATCC 49456), Streptococcus
pyogenes (ATCC 19615), Streptococcus salivarius
(ATCC 25975), Escherichia coli (ATCC 11229 and
25922), Pseudomonas aeruginosa (ATCC 27853) and
Enterobacter aerogenes(ATCC 27853).The crude extract
of O. martiana (32.9 mg mL-1) had antibacterial potential
against all Gram-positive bacteria tested. Analysis
of the bioautograms led to the detection of bioactive
substances, among which it was possible to identify
piperovatine (Rf 0.35), piperlonguminine (Rf 0.52) and
isopiperlonguminine (Rf 0.52). The piperovatine and
isopiperlonguminine were isolated from the roots of
O. martiana, guided by a bioautographic antibacterial
bioassay. |
| first_indexed | 2024-12-19T14:05:24Z |
| format | Article |
| id | doaj.art-57c67882f6e14e6a857b1a8664f630f6 |
| institution | Directory Open Access Journal |
| issn | 1808-4532 |
| language | English |
| last_indexed | 2024-12-19T14:05:24Z |
| publishDate | 2012-12-01 |
| publisher | São Paulo State University (UNESP) |
| record_format | Article |
| series | Revista de Ciências Farmacêuticas Básica e Aplicada |
| spelling | doaj.art-57c67882f6e14e6a857b1a8664f630f62022-12-21T20:18:19ZengSão Paulo State University (UNESP)Revista de Ciências Farmacêuticas Básica e Aplicada1808-45322012-12-01334515519Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiotaMiriam Machado Cunico0Celso Garcia Auer1Cristina Peitz de Lima2Lilian Cristina Côcco3Carlos Itsuo Yamamoto4Marilis Dallarmi Miguel5Obdulio Gomes Miguel6Carlos Roberto Sanquetta7Universidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáOttonia martiana Miq. (Piperaceae), a plant known popularly in southern Brazil as “anestésia” and used in the treatment of odontalgia for its anesthetic action on the oral mucosa, was investigated for antibacterial activity by paper disc agar diffusion and bioautographic methods, against microorganisms present in the human oral cavity [Streptococcus mutans (ATCC 25175), Streptococcus mitis (ATCC 49456), Streptococcus pyogenes (ATCC 19615), Streptococcus salivarius (ATCC 25975), Escherichia coli (ATCC 11229 and 25922), Pseudomonas aeruginosa (ATCC 27853) and Enterobacter aerogenes(ATCC 27853).The crude extract of O. martiana (32.9 mg mL-1) had antibacterial potential against all Gram-positive bacteria tested. Analysis of the bioautograms led to the detection of bioactive substances, among which it was possible to identify piperovatine (Rf 0.35), piperlonguminine (Rf 0.52) and isopiperlonguminine (Rf 0.52). The piperovatine and isopiperlonguminine were isolated from the roots of O. martiana, guided by a bioautographic antibacterial bioassay.http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2398/1325AmidesAntibacterial activityBioautographyToothacheOttonia martiana |
| spellingShingle | Miriam Machado Cunico Celso Garcia Auer Cristina Peitz de Lima Lilian Cristina Côcco Carlos Itsuo Yamamoto Marilis Dallarmi Miguel Obdulio Gomes Miguel Carlos Roberto Sanquetta Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota Revista de Ciências Farmacêuticas Básica e Aplicada Amides Antibacterial activity Bioautography Toothache Ottonia martiana |
| title | Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota |
| title_full | Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota |
| title_fullStr | Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota |
| title_full_unstemmed | Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota |
| title_short | Bioautography to assess antibacterial activity of Ottonia martiana Miq. (Piperaceae) on the human oral microbiota |
| title_sort | bioautography to assess antibacterial activity of ottonia martiana miq piperaceae on the human oral microbiota |
| topic | Amides Antibacterial activity Bioautography Toothache Ottonia martiana |
| url | http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2398/1325 |
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