Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system
Nociception is the sensory perception of noxious chemical stimuli. Repellent behavior to avoid noxious stimuli is indispensable for survival, and this mechanism has been evolutionarily conserved across a wide range of species, from mammals to insects. The transient receptor potential ankyrin 1 (TRPA...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Insect Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266651582300015X |
| _version_ | 1797398493965320192 |
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| author | Kenji Shimomura Hinoki Oikawa Kosuke Yamamoto Takehito Terajima Shunsuke Yajima Motohiro Tomizawa |
| author_facet | Kenji Shimomura Hinoki Oikawa Kosuke Yamamoto Takehito Terajima Shunsuke Yajima Motohiro Tomizawa |
| author_sort | Kenji Shimomura |
| collection | DOAJ |
| description | Nociception is the sensory perception of noxious chemical stimuli. Repellent behavior to avoid noxious stimuli is indispensable for survival, and this mechanism has been evolutionarily conserved across a wide range of species, from mammals to insects. The transient receptor potential ankyrin 1 (TRPA1) channel is one of the most conserved noxious chemical sensors. Here, we describe the heterologous stable expression of Tribolium castaneum TRPA1 (TcTRPA1) in human embryonic kidney (HEK293) cells. The intracellular Ca2+ influx was measured when two compounds, citronellal and l-menthol, derived from plant essential oils, were applied in vitro using a fluorescence assay. The analysis revealed that citronellal evoked Ca2+ influx dose-dependently for TcTRPA1, whereas l-menthol did not. In combination with our present and previous results of the avoidance-behavioral assay at the organism level, we suggest that TcTRPA1 discriminates between these two toxic compounds, and diversification in the chemical nociception selectivity has occurred in TRPA1 channel among insect taxa. |
| first_indexed | 2024-03-09T01:26:18Z |
| format | Article |
| id | doaj.art-57c6bc1acda94defb39e1f8f30d2a28c |
| institution | Directory Open Access Journal |
| issn | 2666-5158 |
| language | English |
| last_indexed | 2024-03-09T01:26:18Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Insect Science |
| spelling | doaj.art-57c6bc1acda94defb39e1f8f30d2a28c2023-12-10T06:18:10ZengElsevierCurrent Research in Insect Science2666-51582023-01-014100066Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression systemKenji Shimomura0Hinoki Oikawa1Kosuke Yamamoto2Takehito Terajima3Shunsuke Yajima4Motohiro Tomizawa5Department of Chemistry for Life Sciences and Agriculture, Tokyo University of Agriculture, Tokyo, 156-8502, Japan; Corresponding author at: 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.Department of Bioscience, Tokyo University of Agriculture, Tokyo, 156-8502, JapanDepartment of Molecular Microbiology, Tokyo University of Agriculture, Tokyo, 156-8502, JapanDepartment of Chemistry for Life Sciences and Agriculture, Tokyo University of Agriculture, Tokyo, 156-8502, JapanDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, 156-8502, JapanDepartment of Chemistry for Life Sciences and Agriculture, Tokyo University of Agriculture, Tokyo, 156-8502, JapanNociception is the sensory perception of noxious chemical stimuli. Repellent behavior to avoid noxious stimuli is indispensable for survival, and this mechanism has been evolutionarily conserved across a wide range of species, from mammals to insects. The transient receptor potential ankyrin 1 (TRPA1) channel is one of the most conserved noxious chemical sensors. Here, we describe the heterologous stable expression of Tribolium castaneum TRPA1 (TcTRPA1) in human embryonic kidney (HEK293) cells. The intracellular Ca2+ influx was measured when two compounds, citronellal and l-menthol, derived from plant essential oils, were applied in vitro using a fluorescence assay. The analysis revealed that citronellal evoked Ca2+ influx dose-dependently for TcTRPA1, whereas l-menthol did not. In combination with our present and previous results of the avoidance-behavioral assay at the organism level, we suggest that TcTRPA1 discriminates between these two toxic compounds, and diversification in the chemical nociception selectivity has occurred in TRPA1 channel among insect taxa.http://www.sciencedirect.com/science/article/pii/S266651582300015XTribolium castaneumTRPA1 channelCitronellall-mentholChemical nociception |
| spellingShingle | Kenji Shimomura Hinoki Oikawa Kosuke Yamamoto Takehito Terajima Shunsuke Yajima Motohiro Tomizawa Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system Current Research in Insect Science Tribolium castaneum TRPA1 channel Citronellal l-menthol Chemical nociception |
| title | Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system |
| title_full | Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system |
| title_fullStr | Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system |
| title_full_unstemmed | Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system |
| title_short | Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system |
| title_sort | noxious chemical discrimination by tribolium castaneum trpa1 channel in the hek293 cell expression system |
| topic | Tribolium castaneum TRPA1 channel Citronellal l-menthol Chemical nociception |
| url | http://www.sciencedirect.com/science/article/pii/S266651582300015X |
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