Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
Abstract Background Black pepper (Piper nigrum L.), an important and long-cultivated spice crop, is native to South India and grown in the tropics. Piperine is the main pungent and bioactive alkaloid in the berries of black pepper, but the molecular mechanism for piperine biosynthesis has not been d...
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-08154-4 |
| _version_ | 1818996422112968704 |
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| author | Yuanhao Ding Yuyuan Mao Yi Cen Lisong Hu Yuefeng Su Xuemin Ma Lu Long Haiyan Hu Chaoyun Hao Jie Luo |
| author_facet | Yuanhao Ding Yuyuan Mao Yi Cen Lisong Hu Yuefeng Su Xuemin Ma Lu Long Haiyan Hu Chaoyun Hao Jie Luo |
| author_sort | Yuanhao Ding |
| collection | DOAJ |
| description | Abstract Background Black pepper (Piper nigrum L.), an important and long-cultivated spice crop, is native to South India and grown in the tropics. Piperine is the main pungent and bioactive alkaloid in the berries of black pepper, but the molecular mechanism for piperine biosynthesis has not been determined. MicroRNAs (miRNAs), which are classical endogenous noncoding small RNAs, play important roles in regulating secondary metabolism in many species, but less is known regarding black pepper or piperine biosynthesis. Results To dissect the functions of miRNAs in secondary metabolism especially in piperine biosynthesis, 110 known miRNAs, 18 novel miRNAs and 1007 individual targets were identified from different tissues of black pepper by small RNA sequencing. qRT-PCR and 5′-RLM-RACE experiments were conducted to validate the reliability of the sequencing data and predicted targets. We found 3 miRNAs along with their targets including miR166-4CL, miR396-PER and miR397-CCR modules that are involved in piperine biosynthesis. Conclusion MiRNA regulation of secondary metabolism is a common phenomenon in plants. Our study revealed new miRNAs that regulate piperine biosynthesis, which are special alkaloids in the piper genus, and they might be useful for future piperine genetic improvement of black pepper. |
| first_indexed | 2024-12-20T21:29:29Z |
| format | Article |
| id | doaj.art-57c9920020ed437e954578fe648001ac |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-20T21:29:29Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-57c9920020ed437e954578fe648001ac2022-12-21T19:26:04ZengBMCBMC Genomics1471-21642021-11-0122111210.1186/s12864-021-08154-4Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)Yuanhao Ding0Yuyuan Mao1Yi Cen2Lisong Hu3Yuefeng Su4Xuemin Ma5Lu Long6Haiyan Hu7Chaoyun Hao8Jie Luo9Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversitySpice and Beverage Research Institute, Chinese Academy of Tropical Agricultural SciencesHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversityState Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Science, Henan UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversitySpice and Beverage Research Institute, Chinese Academy of Tropical Agricultural SciencesHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, School of Tropical Crops, Hainan UniversityAbstract Background Black pepper (Piper nigrum L.), an important and long-cultivated spice crop, is native to South India and grown in the tropics. Piperine is the main pungent and bioactive alkaloid in the berries of black pepper, but the molecular mechanism for piperine biosynthesis has not been determined. MicroRNAs (miRNAs), which are classical endogenous noncoding small RNAs, play important roles in regulating secondary metabolism in many species, but less is known regarding black pepper or piperine biosynthesis. Results To dissect the functions of miRNAs in secondary metabolism especially in piperine biosynthesis, 110 known miRNAs, 18 novel miRNAs and 1007 individual targets were identified from different tissues of black pepper by small RNA sequencing. qRT-PCR and 5′-RLM-RACE experiments were conducted to validate the reliability of the sequencing data and predicted targets. We found 3 miRNAs along with their targets including miR166-4CL, miR396-PER and miR397-CCR modules that are involved in piperine biosynthesis. Conclusion MiRNA regulation of secondary metabolism is a common phenomenon in plants. Our study revealed new miRNAs that regulate piperine biosynthesis, which are special alkaloids in the piper genus, and they might be useful for future piperine genetic improvement of black pepper.https://doi.org/10.1186/s12864-021-08154-4Black peppermiRNASecondary metabolismAlkaloidsPiperine biosynthesis |
| spellingShingle | Yuanhao Ding Yuyuan Mao Yi Cen Lisong Hu Yuefeng Su Xuemin Ma Lu Long Haiyan Hu Chaoyun Hao Jie Luo Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.) BMC Genomics Black pepper miRNA Secondary metabolism Alkaloids Piperine biosynthesis |
| title | Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.) |
| title_full | Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.) |
| title_fullStr | Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.) |
| title_full_unstemmed | Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.) |
| title_short | Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.) |
| title_sort | small rna sequencing reveals various micrornas involved in piperine biosynthesis in black pepper piper nigrum l |
| topic | Black pepper miRNA Secondary metabolism Alkaloids Piperine biosynthesis |
| url | https://doi.org/10.1186/s12864-021-08154-4 |
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