Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers
Mucosal infection by the human papillomavirus (HPV) is responsible for a growing number of malignancies, predominantly represented by cervical cancer and oropharyngeal squamous cell carcinoma. Because of the prevalence of the virus, persistence of infection, and long latency period, novel and low-co...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6694/8/9/85 |
| _version_ | 1797715843376742400 |
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| author | Sunny S. Shah Satyajyoti Senapati Flora Klacsmann Daniel L. Miller Jeff J. Johnson Hsueh-Chia Chang M. Sharon Stack |
| author_facet | Sunny S. Shah Satyajyoti Senapati Flora Klacsmann Daniel L. Miller Jeff J. Johnson Hsueh-Chia Chang M. Sharon Stack |
| author_sort | Sunny S. Shah |
| collection | DOAJ |
| description | Mucosal infection by the human papillomavirus (HPV) is responsible for a growing number of malignancies, predominantly represented by cervical cancer and oropharyngeal squamous cell carcinoma. Because of the prevalence of the virus, persistence of infection, and long latency period, novel and low-cost methods are needed for effective population level screening and monitoring. We review established methods for screening of cervical and oral cancer as well as commercially-available techniques for detection of HPV DNA. We then describe the ongoing development of microfluidic nucleic acid-based biosensors to evaluate circulating host microRNAs that are produced in response to an oncogenic HPV infection. The goal is to develop an ideal screening platform that is low-cost, portable, and easy to use, with appropriate signal stability, sensitivity and specificity. Advances in technologies for sample lysis, pre-treatment and concentration, and multiplexed nucleic acid detection are provided. Continued development of these devices provides opportunities for cancer screening in low resource settings, for point-of-care diagnostics and self-screening, and for monitoring response to vaccination or surgical treatment. |
| first_indexed | 2024-03-12T08:12:47Z |
| format | Article |
| id | doaj.art-57ca41e1779747f0a6da15289cc2ad38 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T08:12:47Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-57ca41e1779747f0a6da15289cc2ad382023-09-02T19:03:53ZengMDPI AGCancers2072-66942016-09-01898510.3390/cancers8090085cancers8090085Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal CancersSunny S. Shah0Satyajyoti Senapati1Flora Klacsmann2Daniel L. Miller3Jeff J. Johnson4Hsueh-Chia Chang5M. Sharon Stack6Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USADepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USABionics Innovation Center Nonprofit Ltd., 1088 Budapest, HungaryDepartment of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USAHarper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USADepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USAHarper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USAMucosal infection by the human papillomavirus (HPV) is responsible for a growing number of malignancies, predominantly represented by cervical cancer and oropharyngeal squamous cell carcinoma. Because of the prevalence of the virus, persistence of infection, and long latency period, novel and low-cost methods are needed for effective population level screening and monitoring. We review established methods for screening of cervical and oral cancer as well as commercially-available techniques for detection of HPV DNA. We then describe the ongoing development of microfluidic nucleic acid-based biosensors to evaluate circulating host microRNAs that are produced in response to an oncogenic HPV infection. The goal is to develop an ideal screening platform that is low-cost, portable, and easy to use, with appropriate signal stability, sensitivity and specificity. Advances in technologies for sample lysis, pre-treatment and concentration, and multiplexed nucleic acid detection are provided. Continued development of these devices provides opportunities for cancer screening in low resource settings, for point-of-care diagnostics and self-screening, and for monitoring response to vaccination or surgical treatment.http://www.mdpi.com/2072-6694/8/9/85human papillomaviruscervical and oropharyngeal cancersdiagnosticsintegrated platformHPV DNAE6/E7 genotypesmicroRNA |
| spellingShingle | Sunny S. Shah Satyajyoti Senapati Flora Klacsmann Daniel L. Miller Jeff J. Johnson Hsueh-Chia Chang M. Sharon Stack Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers Cancers human papillomavirus cervical and oropharyngeal cancers diagnostics integrated platform HPV DNA E6/E7 genotypes microRNA |
| title | Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers |
| title_full | Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers |
| title_fullStr | Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers |
| title_full_unstemmed | Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers |
| title_short | Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers |
| title_sort | current technologies and recent developments for screening of hpv associated cervical and oropharyngeal cancers |
| topic | human papillomavirus cervical and oropharyngeal cancers diagnostics integrated platform HPV DNA E6/E7 genotypes microRNA |
| url | http://www.mdpi.com/2072-6694/8/9/85 |
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