Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method
Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup> were developed from <i>Macleaya</i><i>cordata</i> and are widely used in agriculture and animal husbandry, but their impurities have been rarely reported in the literature. Impurity analysis is of great...
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/13/3851 |
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| author | Zhuang Dong Mengting Liu Xiaohong Zhong Xiaoyong Ou Xuan Yun Mingcan Wang Shurui Ren Zhixing Qing Jianguo Zeng |
| author_facet | Zhuang Dong Mengting Liu Xiaohong Zhong Xiaoyong Ou Xuan Yun Mingcan Wang Shurui Ren Zhixing Qing Jianguo Zeng |
| author_sort | Zhuang Dong |
| collection | DOAJ |
| description | Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup> were developed from <i>Macleaya</i><i>cordata</i> and are widely used in agriculture and animal husbandry, but their impurities have been rarely reported in the literature. Impurity analysis is of great importance to the quality and safety of veterinary drugs. In this study, high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening method was used to screen and characterize the impurities in Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup>. A total of 58 impurities were screened from Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup> using the screening strategies, of which 39 were identified by their accurate <i>m/z</i> value, characteristic MS/MS data, and fragmentation pathways of references. This established method was used for impurity analysis for the first time and proved to be a useful and rapid tool to screen and identify the impurities of Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup>, especially for those at trace levels in a complex sample. In addition, this study marks the first comprehensive research into impurities in these two products and has great significance for the systematic detection of impurities in other plant-derived drugs. |
| first_indexed | 2024-03-10T10:05:30Z |
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| id | doaj.art-57cacb550a864912a1c4da85a78c080d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T11:27:40Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-57cacb550a864912a1c4da85a78c080d2024-04-10T12:59:35ZengMDPI AGMolecules1420-30492021-06-012613385110.3390/molecules26133851Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening MethodZhuang Dong0Mengting Liu1Xiaohong Zhong2Xiaoyong Ou3Xuan Yun4Mingcan Wang5Shurui Ren6Zhixing Qing7Jianguo Zeng8Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaBopu powder<sup>®</sup> and Sangrovit<sup>®</sup> were developed from <i>Macleaya</i><i>cordata</i> and are widely used in agriculture and animal husbandry, but their impurities have been rarely reported in the literature. Impurity analysis is of great importance to the quality and safety of veterinary drugs. In this study, high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening method was used to screen and characterize the impurities in Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup>. A total of 58 impurities were screened from Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup> using the screening strategies, of which 39 were identified by their accurate <i>m/z</i> value, characteristic MS/MS data, and fragmentation pathways of references. This established method was used for impurity analysis for the first time and proved to be a useful and rapid tool to screen and identify the impurities of Bopu powder<sup>®</sup> and Sangrovit<sup>®</sup>, especially for those at trace levels in a complex sample. In addition, this study marks the first comprehensive research into impurities in these two products and has great significance for the systematic detection of impurities in other plant-derived drugs.https://www.mdpi.com/1420-3049/26/13/3851impurity analysisBopu powder<sup>®</sup>Sangrovit<sup>®</sup>isoquinoline alkaloidsscreening methodLC-MS |
| spellingShingle | Zhuang Dong Mengting Liu Xiaohong Zhong Xiaoyong Ou Xuan Yun Mingcan Wang Shurui Ren Zhixing Qing Jianguo Zeng Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method Molecules impurity analysis Bopu powder<sup>®</sup> Sangrovit<sup>®</sup> isoquinoline alkaloids screening method LC-MS |
| title | Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method |
| title_full | Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method |
| title_fullStr | Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method |
| title_full_unstemmed | Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method |
| title_short | Identification of the Trace Components in BopuzongJian and <em>Macleaya cordata</em> Extract Using LC-MS Combined with a Screening Method |
| title_sort | identification of the trace components in bopuzongjian and em macleaya cordata em extract using lc ms combined with a screening method |
| topic | impurity analysis Bopu powder<sup>®</sup> Sangrovit<sup>®</sup> isoquinoline alkaloids screening method LC-MS |
| url | https://www.mdpi.com/1420-3049/26/13/3851 |
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