Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report
Toll-like receptors (TLRs) are known to have an important role in triggering the innate immune response and in priming antigen-specific adaptive immunity and inflammation. The differences in synovial tissue expression of the TLRs between seronegative and seropositive rheumatoid arthritis (RA) were e...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Autoimmunity |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08916934.2020.1864729 |
| _version_ | 1827817281501003776 |
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| author | Alzahraa Abdelwahab Sanna Palosaari Soha Abdelkawy Abdelwahab Rehab Ahmed Rifaai Nashwa Fathy El-Tahawy Entesar Ali Saber Tomi Nousiainen Maarit Valkealahti Johanna Huhtakangas Tuomo J. Karttunen Petri Lehenkari |
| author_facet | Alzahraa Abdelwahab Sanna Palosaari Soha Abdelkawy Abdelwahab Rehab Ahmed Rifaai Nashwa Fathy El-Tahawy Entesar Ali Saber Tomi Nousiainen Maarit Valkealahti Johanna Huhtakangas Tuomo J. Karttunen Petri Lehenkari |
| author_sort | Alzahraa Abdelwahab |
| collection | DOAJ |
| description | Toll-like receptors (TLRs) are known to have an important role in triggering the innate immune response and in priming antigen-specific adaptive immunity and inflammation. The differences in synovial tissue expression of the TLRs between seronegative and seropositive rheumatoid arthritis (RA) were examined from 9 seropositive RA, 5 seronegative RA and 4 osteoarthritis (OA) patients. Synovitis status was assessed using Krenn’s scoring and TLR 1–9 expression by immunohistochemistry. Tissue citrulline content was analysed by HPLC method. In RA TLR expression was generally higher than in OA. TLR2 expression was higher in both seronegative and seropositive RA compared to OA. TLR 1, 4 and 8 expressions were higher in seropositive RA than in seronegative RA or in OA. For TLRs 3, 5, 6, 7 and 9 local differences of expression were found between groups. TLR 1–9 expression correlated with the synovitis grade. No statistical difference was found in synovial tissue citrulline content between the groups. In seropositive RA, the TLR repertoire in the synovial tissue differs from seronegative RA and could explain differences in disease outcomes. The high expression of protein sensing (TLR1, TLR2 and TLR4) and nucleic acid sensing TLRs (TLR7, TLR8 and TLR9) in the seropositive RA could make the synovium primed for reacting to citrullinated proteins and nucleic acids that could be released to extracellular space in formation of neutrophil extracellular traps. This reactivity could be augmented by Fc receptor activation by anti-citrullinated protein antibody immunocomplexes associated with seropositive RA. |
| first_indexed | 2024-03-12T00:32:09Z |
| format | Article |
| id | doaj.art-57cb839778d944a4a70aa7edd72a0363 |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:32:09Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-57cb839778d944a4a70aa7edd72a03632023-09-15T10:12:23ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2021-01-01541233410.1080/08916934.2020.18647291864729Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary reportAlzahraa Abdelwahab0Sanna Palosaari1Soha Abdelkawy Abdelwahab2Rehab Ahmed Rifaai3Nashwa Fathy El-Tahawy4Entesar Ali Saber5Tomi Nousiainen6Maarit Valkealahti7Johanna Huhtakangas8Tuomo J. Karttunen9Petri Lehenkari10Department of Histology and Cell Biology, Faculty of Medicine, Minia UniversityAnatomy and Cell Biology, Medical Research Center Oulu and Cancer and Translational Medicine Research Unit, Oulu University Hospital and University of OuluDepartment of Histology and Cell Biology, Faculty of Medicine, Minia University, delegated to Deraya UniversityDepartment of Histology and Cell Biology, Faculty of Medicine, Minia UniversityDepartment of Histology and Cell Biology, Faculty of Medicine, Minia UniversityDepartment of Histology and Cell Biology, Faculty of Medicine, Minia University, delegated to Deraya UniversityDepartment of Surgery, Oulu University HospitalDepartment of Surgery, Oulu University HospitalAnatomy and Cell Biology, Medical Research Center Oulu and Cancer and Translational Medicine Research Unit, Oulu University Hospital and University of OuluDepartment of Pathology, Cancer and Translational Medicine Medical Research Unit, and Medical Research Center Oulu, Oulu University Hospital and University of OuluAnatomy and Cell Biology, Medical Research Center Oulu and Cancer and Translational Medicine Research Unit, Oulu University Hospital and University of OuluToll-like receptors (TLRs) are known to have an important role in triggering the innate immune response and in priming antigen-specific adaptive immunity and inflammation. The differences in synovial tissue expression of the TLRs between seronegative and seropositive rheumatoid arthritis (RA) were examined from 9 seropositive RA, 5 seronegative RA and 4 osteoarthritis (OA) patients. Synovitis status was assessed using Krenn’s scoring and TLR 1–9 expression by immunohistochemistry. Tissue citrulline content was analysed by HPLC method. In RA TLR expression was generally higher than in OA. TLR2 expression was higher in both seronegative and seropositive RA compared to OA. TLR 1, 4 and 8 expressions were higher in seropositive RA than in seronegative RA or in OA. For TLRs 3, 5, 6, 7 and 9 local differences of expression were found between groups. TLR 1–9 expression correlated with the synovitis grade. No statistical difference was found in synovial tissue citrulline content between the groups. In seropositive RA, the TLR repertoire in the synovial tissue differs from seronegative RA and could explain differences in disease outcomes. The high expression of protein sensing (TLR1, TLR2 and TLR4) and nucleic acid sensing TLRs (TLR7, TLR8 and TLR9) in the seropositive RA could make the synovium primed for reacting to citrullinated proteins and nucleic acids that could be released to extracellular space in formation of neutrophil extracellular traps. This reactivity could be augmented by Fc receptor activation by anti-citrullinated protein antibody immunocomplexes associated with seropositive RA.http://dx.doi.org/10.1080/08916934.2020.1864729immunohistochemistryosteoarthritisrheumatoid arthritisanti-ccptoll-like receptortlr |
| spellingShingle | Alzahraa Abdelwahab Sanna Palosaari Soha Abdelkawy Abdelwahab Rehab Ahmed Rifaai Nashwa Fathy El-Tahawy Entesar Ali Saber Tomi Nousiainen Maarit Valkealahti Johanna Huhtakangas Tuomo J. Karttunen Petri Lehenkari Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report Autoimmunity immunohistochemistry osteoarthritis rheumatoid arthritis anti-ccp toll-like receptor tlr |
| title | Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report |
| title_full | Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report |
| title_fullStr | Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report |
| title_full_unstemmed | Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report |
| title_short | Differential synovial tissue expression of TLRs in seropositive and seronegative rheumatoid arthritis: A preliminary report |
| title_sort | differential synovial tissue expression of tlrs in seropositive and seronegative rheumatoid arthritis a preliminary report |
| topic | immunohistochemistry osteoarthritis rheumatoid arthritis anti-ccp toll-like receptor tlr |
| url | http://dx.doi.org/10.1080/08916934.2020.1864729 |
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