Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes
Ultraviolet (UV) light is an effective disinfection method. In particular, UV light-emitting diodes (UV-LEDs) are expected to have many applications as light sources owing to their compact form factor and wide range of choices of wavelengths. However, the UV sensitivity of microorganisms for each UV...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402403487X |
| _version_ | 1797224263227277312 |
|---|---|
| author | Kai Ishida Yushi Onoda Yasuko Kadomura-Ishikawa Miharu Nagahashi Michiyo Yamashita Shiho Fukushima Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Takashi Uebanso Masatake Akutagawa Kazuaki Mawatari Akira Takahashi |
| author_facet | Kai Ishida Yushi Onoda Yasuko Kadomura-Ishikawa Miharu Nagahashi Michiyo Yamashita Shiho Fukushima Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Takashi Uebanso Masatake Akutagawa Kazuaki Mawatari Akira Takahashi |
| author_sort | Kai Ishida |
| collection | DOAJ |
| description | Ultraviolet (UV) light is an effective disinfection method. In particular, UV light-emitting diodes (UV-LEDs) are expected to have many applications as light sources owing to their compact form factor and wide range of choices of wavelengths. However, the UV sensitivity of microorganisms for each UV wavelength has not been evaluated comprehensively because standard experimental conditions based on LED characteristics have not been established. Therefore, it is necessary to establish a standard evaluation method based on LED characteristics. Here, we developed a new UV-LED device based on strictly controlled irradiation conditions using LEDs for each wavelength (250–365 nm), checked the validity of the device characteristics and evaluated the UV sensitivity of Escherichia coli using this new evaluation method. For this new device, we considered accurate irradiance, accurate spectra, irradiance uniformity, accurate dose, beam angle, surrounding material reflections, and sample condition. From our results, the following UV irradiation conditions were established as standard: 1 mW/cm2 irradiance, bacterial solution with absorbance value of A600 = 0.5 diluted 10 times solution, solution volume of 1 mL, working distance (WD) of 100 mm. In order to compare the effects of irradiation under uniform conditions on inactivation of microorganisms, we assessed inactivation effect of E. coli by LED irradiation at each wavelength using the U280 LED as a standard wavelength. The inactivation effect for U280 LED irradiation was −0.95 ± 0.21 log at a dose of 4 mJ/cm2. Under this condition of dose, our results showed a high wavelength dependence of the inactivation effect at each UV wavelength peaking at 267 nm. Our study showed that this irradiation system was validated for the standard UV irradiation system and could be contributed to the establishment of food and water hygiene control methods and the development of equipment for the prevention of infectious diseases. |
| first_indexed | 2024-04-24T13:50:20Z |
| format | Article |
| id | doaj.art-57cfd862df774078ad5d6ea5d333887e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:20Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-57cfd862df774078ad5d6ea5d333887e2024-04-04T05:04:53ZengElsevierHeliyon2405-84402024-03-01106e27456Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodesKai Ishida0Yushi Onoda1Yasuko Kadomura-Ishikawa2Miharu Nagahashi3Michiyo Yamashita4Shiho Fukushima5Toshihiko Aizawa6Shigeharu Yamauchi7Yasuo Fujikawa8Tomotake Tanaka9Takashi Uebanso10Masatake Akutagawa11Kazuaki Mawatari12Akira Takahashi13Department of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Nichia Corporation, Tokushima, JapanDepartment of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanNichia Corporation, Tokushima, JapanNichia Corporation, Tokushima, JapanNichia Corporation, Tokushima, JapanNichia Corporation, Tokushima, JapanDepartment of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Department of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Electrical and Electronic Engineering, Graduate School of Technology, Industrial and Social Sciences, University of Tokushima, Tokushima, JapanDepartment of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Department of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Microbial Control, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Department of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Corresponding author. Department of Preventive Environment and Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Japan.Ultraviolet (UV) light is an effective disinfection method. In particular, UV light-emitting diodes (UV-LEDs) are expected to have many applications as light sources owing to their compact form factor and wide range of choices of wavelengths. However, the UV sensitivity of microorganisms for each UV wavelength has not been evaluated comprehensively because standard experimental conditions based on LED characteristics have not been established. Therefore, it is necessary to establish a standard evaluation method based on LED characteristics. Here, we developed a new UV-LED device based on strictly controlled irradiation conditions using LEDs for each wavelength (250–365 nm), checked the validity of the device characteristics and evaluated the UV sensitivity of Escherichia coli using this new evaluation method. For this new device, we considered accurate irradiance, accurate spectra, irradiance uniformity, accurate dose, beam angle, surrounding material reflections, and sample condition. From our results, the following UV irradiation conditions were established as standard: 1 mW/cm2 irradiance, bacterial solution with absorbance value of A600 = 0.5 diluted 10 times solution, solution volume of 1 mL, working distance (WD) of 100 mm. In order to compare the effects of irradiation under uniform conditions on inactivation of microorganisms, we assessed inactivation effect of E. coli by LED irradiation at each wavelength using the U280 LED as a standard wavelength. The inactivation effect for U280 LED irradiation was −0.95 ± 0.21 log at a dose of 4 mJ/cm2. Under this condition of dose, our results showed a high wavelength dependence of the inactivation effect at each UV wavelength peaking at 267 nm. Our study showed that this irradiation system was validated for the standard UV irradiation system and could be contributed to the establishment of food and water hygiene control methods and the development of equipment for the prevention of infectious diseases.http://www.sciencedirect.com/science/article/pii/S240584402403487XUV-LEDsEscherichia coliUV doseStandard evaluation methodMicroorganism inactivation |
| spellingShingle | Kai Ishida Yushi Onoda Yasuko Kadomura-Ishikawa Miharu Nagahashi Michiyo Yamashita Shiho Fukushima Toshihiko Aizawa Shigeharu Yamauchi Yasuo Fujikawa Tomotake Tanaka Takashi Uebanso Masatake Akutagawa Kazuaki Mawatari Akira Takahashi Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes Heliyon UV-LEDs Escherichia coli UV dose Standard evaluation method Microorganism inactivation |
| title | Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes |
| title_full | Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes |
| title_fullStr | Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes |
| title_full_unstemmed | Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes |
| title_short | Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes |
| title_sort | development of a standard evaluation method for microbial uv sensitivity using light emitting diodes |
| topic | UV-LEDs Escherichia coli UV dose Standard evaluation method Microorganism inactivation |
| url | http://www.sciencedirect.com/science/article/pii/S240584402403487X |
| work_keys_str_mv | AT kaiishida developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT yushionoda developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT yasukokadomuraishikawa developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT miharunagahashi developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT michiyoyamashita developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT shihofukushima developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT toshihikoaizawa developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT shigeharuyamauchi developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT yasuofujikawa developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT tomotaketanaka developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT takashiuebanso developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT masatakeakutagawa developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT kazuakimawatari developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes AT akiratakahashi developmentofastandardevaluationmethodformicrobialuvsensitivityusinglightemittingdiodes |