The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia
Introdution: Muscle weakness that is caused by cancer called Cachexia. One of the causes of the formation of the cachexia is the change in protein degradation, and the ubiquitous protease and Autophagy Lysosomes system is the most important protein breakdown system. Resistance training has been one...
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2019-07-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/article-1-4706-en.html |
| _version_ | 1811310229318533120 |
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| author | Mahdi Samadi Zahra Mojtahedi Farhad Daryanoosh Javad Nemati |
| author_facet | Mahdi Samadi Zahra Mojtahedi Farhad Daryanoosh Javad Nemati |
| author_sort | Mahdi Samadi |
| collection | DOAJ |
| description | Introdution: Muscle weakness that is caused by cancer called Cachexia. One of the causes of the formation of the cachexia is the change in protein degradation, and the ubiquitous protease and Autophagy Lysosomes system is the most important protein breakdown system. Resistance training has been one the best stimulator of increasing muscular mass. Therefore, the purpose of this study was to investigate the effect of a period of resistance training on the muscle protease activity and autophagy flux in mice with cancer induce cachexia.
Methods: This study was conducted on 12 male BALB / c mice (age 6 weeks) and CT-26 tumor implanted into them. Then they were divided into two groups of resistance training (n=6) and control (n=6). Training group performed 6 weeks progressive resistance training. The protease activity was evaluated by fluorogenic substrate method and LC3B and p62 evaluated by western blot method. Independent t-test was carried out at P<0.05 for statistical analysis using Prism (7) software.
Results: There was no significant difference in Proteasome activity (p=0.13), muscle weight (p=0.24), and CT26 tumor volume (p≥0.05) between two groups of resistance training and control, but p62 decreased in the resistance training group (p=0.032).
Conclusion: However, the autophagy flux has improved in the part of cargo breakdown with resistance training, but perhaps the presence of a tumor inhibits muscle response to resistance training or this type of resistance training is not an appropriate intervention for treating cachectic muscle. To treat cachexia through the resistance training, either this method would be combined with other interventions or other methods of the resistance training can be applied. |
| first_indexed | 2024-04-13T09:55:28Z |
| format | Article |
| id | doaj.art-57d087f04bbf4f4daa376ece1faf11c0 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-04-13T09:55:28Z |
| publishDate | 2019-07-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-57d087f04bbf4f4daa376ece1faf11c02022-12-22T02:51:24ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332019-07-0127313131326The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexiaMahdi Samadi0Zahra Mojtahedi1Farhad Daryanoosh2Javad Nemati3 School of Education and Psychology, Department of Sport Sciences, Shiraz University Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences School of Education and Psychology, Department of Sport Sciences, Shiraz University School of Education and Psychology, Department of Sport Sciences, Shiraz University, Introdution: Muscle weakness that is caused by cancer called Cachexia. One of the causes of the formation of the cachexia is the change in protein degradation, and the ubiquitous protease and Autophagy Lysosomes system is the most important protein breakdown system. Resistance training has been one the best stimulator of increasing muscular mass. Therefore, the purpose of this study was to investigate the effect of a period of resistance training on the muscle protease activity and autophagy flux in mice with cancer induce cachexia. Methods: This study was conducted on 12 male BALB / c mice (age 6 weeks) and CT-26 tumor implanted into them. Then they were divided into two groups of resistance training (n=6) and control (n=6). Training group performed 6 weeks progressive resistance training. The protease activity was evaluated by fluorogenic substrate method and LC3B and p62 evaluated by western blot method. Independent t-test was carried out at P<0.05 for statistical analysis using Prism (7) software. Results: There was no significant difference in Proteasome activity (p=0.13), muscle weight (p=0.24), and CT26 tumor volume (p≥0.05) between two groups of resistance training and control, but p62 decreased in the resistance training group (p=0.032). Conclusion: However, the autophagy flux has improved in the part of cargo breakdown with resistance training, but perhaps the presence of a tumor inhibits muscle response to resistance training or this type of resistance training is not an appropriate intervention for treating cachectic muscle. To treat cachexia through the resistance training, either this method would be combined with other interventions or other methods of the resistance training can be applied.http://jssu.ssu.ac.ir/article-1-4706-en.htmlCancer CachexiaProteasome activityResistance trainingAutophagy flux |
| spellingShingle | Mahdi Samadi Zahra Mojtahedi Farhad Daryanoosh Javad Nemati The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Cancer Cachexia Proteasome activity Resistance training Autophagy flux |
| title | The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia |
| title_full | The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia |
| title_fullStr | The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia |
| title_full_unstemmed | The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia |
| title_short | The effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia |
| title_sort | effect of a period of resistance training on the muscle proteasome activity and autophgy flux in mice with cancer induce cachexia |
| topic | Cancer Cachexia Proteasome activity Resistance training Autophagy flux |
| url | http://jssu.ssu.ac.ir/article-1-4706-en.html |
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