Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn
Abstract The existence of purple-pericarp super-sweetcorn based on the supersweet mutation, shrunken2 (sh2), has not been previously reported, due to its extremely tight genetic linkage to a non-functional anthocyanin biosynthesis gene, anthocyaninless1 (a1). Generally, pericarp-pigmented starchy pu...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28083-4 |
| _version_ | 1797945947626405888 |
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| author | Apurba Anirban Alice Hayward Hung T. Hong Ardashir Kharabian Masouleh Robert J. Henry Tim J. O’Hare |
| author_facet | Apurba Anirban Alice Hayward Hung T. Hong Ardashir Kharabian Masouleh Robert J. Henry Tim J. O’Hare |
| author_sort | Apurba Anirban |
| collection | DOAJ |
| description | Abstract The existence of purple-pericarp super-sweetcorn based on the supersweet mutation, shrunken2 (sh2), has not been previously reported, due to its extremely tight genetic linkage to a non-functional anthocyanin biosynthesis gene, anthocyaninless1 (a1). Generally, pericarp-pigmented starchy purple corn contains significantly higher anthocyanin. The development of purple-pericarp super-sweetcorn is dependent on breaking the a1–sh2 tight genetic linkage, which occurs at a very low frequency of < 1 in 1000 meiotic crossovers. Here, to develop purple-pericarp super-sweetcorn, an initial cross between a male purple-pericarp maize, ‘Costa Rica’ (A1Sh2.A1Sh2) and a female white shrunken2 super-sweetcorn, ‘Tims-white’ (a1sh2.a1sh2), was conducted. Subsequent self-pollination based on purple-pericarp-shrunken kernels identified a small frequency (0.08%) of initial heterozygous F3 segregants (A1a1.sh2sh2) producing a fully sh2 cob with a purple-pericarp phenotype, enabled by breaking the close genetic linkage between the a1 and sh2 genes. Resulting rounds of self-pollination generated a F6 homozygous purple-pericarp super-sweetcorn (A1A1.sh2sh2) line, ‘Tim1’. Genome sequencing revealed a recombination break between the a1 and yz1 genes of the a1–yz1-x1–sh2 multigenic interval. The novel purple-pericarp super-sweetcorn produced a similar concentration of anthocyanin and sugar as in its purple-pericarp maize and white super-sweetcorn parents, respectively, potentially adding a broader range of health benefits than currently exists with standard yellow/white sweetcorn. |
| first_indexed | 2024-04-10T21:03:07Z |
| format | Article |
| id | doaj.art-57d1112097284f128e8f3eb40f880760 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T21:03:07Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-57d1112097284f128e8f3eb40f8807602023-01-22T12:09:58ZengNature PortfolioScientific Reports2045-23222023-01-0113111310.1038/s41598-023-28083-4Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcornApurba Anirban0Alice Hayward1Hung T. Hong2Ardashir Kharabian Masouleh3Robert J. Henry4Tim J. O’Hare5Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture & Food Innovation (QAAFI), The University of QueenslandCentre for Horticulture Science, QAAFI, The University of QueenslandCentre for Nutrition and Food Sciences, Queensland Alliance for Agriculture & Food Innovation (QAAFI), The University of QueenslandCentre for Crop Science, QAAFI, The University of QueenslandCentre for Crop Science, QAAFI, The University of QueenslandCentre for Nutrition and Food Sciences, Queensland Alliance for Agriculture & Food Innovation (QAAFI), The University of QueenslandAbstract The existence of purple-pericarp super-sweetcorn based on the supersweet mutation, shrunken2 (sh2), has not been previously reported, due to its extremely tight genetic linkage to a non-functional anthocyanin biosynthesis gene, anthocyaninless1 (a1). Generally, pericarp-pigmented starchy purple corn contains significantly higher anthocyanin. The development of purple-pericarp super-sweetcorn is dependent on breaking the a1–sh2 tight genetic linkage, which occurs at a very low frequency of < 1 in 1000 meiotic crossovers. Here, to develop purple-pericarp super-sweetcorn, an initial cross between a male purple-pericarp maize, ‘Costa Rica’ (A1Sh2.A1Sh2) and a female white shrunken2 super-sweetcorn, ‘Tims-white’ (a1sh2.a1sh2), was conducted. Subsequent self-pollination based on purple-pericarp-shrunken kernels identified a small frequency (0.08%) of initial heterozygous F3 segregants (A1a1.sh2sh2) producing a fully sh2 cob with a purple-pericarp phenotype, enabled by breaking the close genetic linkage between the a1 and sh2 genes. Resulting rounds of self-pollination generated a F6 homozygous purple-pericarp super-sweetcorn (A1A1.sh2sh2) line, ‘Tim1’. Genome sequencing revealed a recombination break between the a1 and yz1 genes of the a1–yz1-x1–sh2 multigenic interval. The novel purple-pericarp super-sweetcorn produced a similar concentration of anthocyanin and sugar as in its purple-pericarp maize and white super-sweetcorn parents, respectively, potentially adding a broader range of health benefits than currently exists with standard yellow/white sweetcorn.https://doi.org/10.1038/s41598-023-28083-4 |
| spellingShingle | Apurba Anirban Alice Hayward Hung T. Hong Ardashir Kharabian Masouleh Robert J. Henry Tim J. O’Hare Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn Scientific Reports |
| title | Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn |
| title_full | Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn |
| title_fullStr | Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn |
| title_full_unstemmed | Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn |
| title_short | Breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin-rich purple-pericarp super-sweetcorn |
| title_sort | breaking the tight genetic linkage between the a1 and sh2 genes led to the development of anthocyanin rich purple pericarp super sweetcorn |
| url | https://doi.org/10.1038/s41598-023-28083-4 |
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