Transparent Cross-Flow Platform as Chemiluminescence Detection Cell in Cross Injection Analysis
This work presents the use of a transparent ‘Cross Injection Analysis’ (CIA) platform as a flow system for chemiluminescence (CL) measurements. The CL-CIA flow device incorporates introduction channels for samples and reagents, and the reaction and detection channels are in one acrylic unit. A photo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/3/1316 |
| Summary: | This work presents the use of a transparent ‘Cross Injection Analysis’ (CIA) platform as a flow system for chemiluminescence (CL) measurements. The CL-CIA flow device incorporates introduction channels for samples and reagents, and the reaction and detection channels are in one acrylic unit. A photomultiplier tube placed above the reaction channel detects the emitted luminescence. The system was applied to the analysis of (i) Co(II) via the Co(II)-catalyzed H<sub>2</sub>O<sub>2</sub>-luminol reaction and (ii) paracetamol via its inhibitory effect on the catalytic activity of Fe(CN)<sub>6</sub><sup>3−</sup> on the H<sub>2</sub>O<sub>2</sub>-luminol reaction. A linear calibration was obtained for Co(II) in the range of 0.002 to 0.025 mg L<sup>−1</sup> Co(II) (r<sup>2</sup> = 0.9977) for the determination of Co(II) in water samples. The linear calibration obtained for the paracetamol was 10 to 200 mg L<sup>−1</sup> (r<sup>2</sup> = 0.9906) for the determination of pharmaceutical products. The sample throughput was 60 samples h<sup>−1</sup>. The precision was ≤4.2% RSD. The consumption of the samples and reagents was ca. 170 µL per analysis cycle. |
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| ISSN: | 1420-3049 |