Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance
Abstract Without any realistic prospect of comprehensive global vaccine coverage and lasting immunity, control of pandemics such as COVID-19 will require implementation of large-scale, rapid identification and isolation of infectious individuals to limit further transmission. Here, we describe an au...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-08263-4 |
| _version_ | 1819045620538671104 |
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| author | Robert E. Dewhurst Tatjana Heinrich Paul Watt Paul Ostergaard Jose M. Marimon Mariana Moreira Philip E. Houldsworth Jack D. Rudrum David Wood Sulev Kõks |
| author_facet | Robert E. Dewhurst Tatjana Heinrich Paul Watt Paul Ostergaard Jose M. Marimon Mariana Moreira Philip E. Houldsworth Jack D. Rudrum David Wood Sulev Kõks |
| author_sort | Robert E. Dewhurst |
| collection | DOAJ |
| description | Abstract Without any realistic prospect of comprehensive global vaccine coverage and lasting immunity, control of pandemics such as COVID-19 will require implementation of large-scale, rapid identification and isolation of infectious individuals to limit further transmission. Here, we describe an automated, high-throughput integrated screening platform, incorporating saliva-based loop-mediated isothermal amplification (LAMP) technology, that is designed for population-scale sensitive detection of infectious carriers of SARS-CoV-2 RNA. Central to this surveillance system is the “Sentinel” testing instrument, which is capable of reporting results within 25 min of saliva sample collection with a throughput of up to 3840 results per hour. It incorporates continuous flow loading of samples at random intervals to cost-effectively adjust for fluctuations in testing demand. Independent validation of our saliva-based RT-LAMP technology on an automated LAMP instrument coined the “Sentinel”, found 98.7% sensitivity, 97.6% specificity, and 98% accuracy against a RT-PCR comparator assay, confirming its suitability for surveillance screening. This Sentinel surveillance system offers a feasible and scalable approach to complement vaccination, to curb the spread of COVID-19 variants, and control future pandemics to save lives. |
| first_indexed | 2024-12-21T10:31:28Z |
| format | Article |
| id | doaj.art-57d6b09f937f42d49a5db80c3dc569c0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T10:31:28Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-57d6b09f937f42d49a5db80c3dc569c02022-12-21T19:07:11ZengNature PortfolioScientific Reports2045-23222022-04-0112111510.1038/s41598-022-08263-4Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillanceRobert E. Dewhurst0Tatjana Heinrich1Paul Watt2Paul Ostergaard3Jose M. Marimon4Mariana Moreira5Philip E. Houldsworth6Jack D. Rudrum7David Wood8Sulev Kõks9Perron Institute for Neurological and Translational SciencePerron Institute for Neurological and Translational ScienceAvicena Systems LtdAvicena Systems LtdBiodonostia Health Research Institute, Infectious Diseases Area, Osakidetza Basque Health Service, Donostialdea Integrated Health OrganizationLancs Lamp LaboratoryLancs Lamp LaboratoryPerron Institute for Neurological and Translational ScienceUniversity of Western AustraliaPerron Institute for Neurological and Translational ScienceAbstract Without any realistic prospect of comprehensive global vaccine coverage and lasting immunity, control of pandemics such as COVID-19 will require implementation of large-scale, rapid identification and isolation of infectious individuals to limit further transmission. Here, we describe an automated, high-throughput integrated screening platform, incorporating saliva-based loop-mediated isothermal amplification (LAMP) technology, that is designed for population-scale sensitive detection of infectious carriers of SARS-CoV-2 RNA. Central to this surveillance system is the “Sentinel” testing instrument, which is capable of reporting results within 25 min of saliva sample collection with a throughput of up to 3840 results per hour. It incorporates continuous flow loading of samples at random intervals to cost-effectively adjust for fluctuations in testing demand. Independent validation of our saliva-based RT-LAMP technology on an automated LAMP instrument coined the “Sentinel”, found 98.7% sensitivity, 97.6% specificity, and 98% accuracy against a RT-PCR comparator assay, confirming its suitability for surveillance screening. This Sentinel surveillance system offers a feasible and scalable approach to complement vaccination, to curb the spread of COVID-19 variants, and control future pandemics to save lives.https://doi.org/10.1038/s41598-022-08263-4 |
| spellingShingle | Robert E. Dewhurst Tatjana Heinrich Paul Watt Paul Ostergaard Jose M. Marimon Mariana Moreira Philip E. Houldsworth Jack D. Rudrum David Wood Sulev Kõks Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance Scientific Reports |
| title | Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance |
| title_full | Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance |
| title_fullStr | Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance |
| title_full_unstemmed | Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance |
| title_short | Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance |
| title_sort | validation of a rapid saliva based and ultra sensitive sars cov 2 screening system for pandemic scale infection surveillance |
| url | https://doi.org/10.1038/s41598-022-08263-4 |
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