Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice.
A strain of human CD3ε transgenic mice, tgε26, exhibits severe immunodeficiency associated with early arrest of T cell development. Complete loss of T cells is observed in homozygous tgε26 mice, but not in heterozygotes, suggesting that genomic disruption due to transgenic integration may contribute...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3008721?pdf=render |
| _version_ | 1818509334246588416 |
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| author | Izumi Ohigashi Yuki Yamasaki Tsukasa Hirashima Yousuke Takahama |
| author_facet | Izumi Ohigashi Yuki Yamasaki Tsukasa Hirashima Yousuke Takahama |
| author_sort | Izumi Ohigashi |
| collection | DOAJ |
| description | A strain of human CD3ε transgenic mice, tgε26, exhibits severe immunodeficiency associated with early arrest of T cell development. Complete loss of T cells is observed in homozygous tgε26 mice, but not in heterozygotes, suggesting that genomic disruption due to transgenic integration may contribute to the arrest of T cell development. Here we report the identification of the transgenic integration site in tgε26 mice. We found that multiple copies of the human CD3ε transgene are inserted between the Sstr5 and Metrn loci on chromosome 17, and that this is accompanied by duplication of the neighboring genomic region spanning 323 kb. However, none of the genes in this region were abrogated. These results suggest that the severe immunodeficiency seen in tgε26 mice is not due to gene disruption resulting from transgenic integration. |
| first_indexed | 2024-12-10T22:44:09Z |
| format | Article |
| id | doaj.art-57d6d1f576c6421198eed80dcb518394 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:44:09Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-57d6d1f576c6421198eed80dcb5183942022-12-22T01:30:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01512e1439110.1371/journal.pone.0014391Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice.Izumi OhigashiYuki YamasakiTsukasa HirashimaYousuke TakahamaA strain of human CD3ε transgenic mice, tgε26, exhibits severe immunodeficiency associated with early arrest of T cell development. Complete loss of T cells is observed in homozygous tgε26 mice, but not in heterozygotes, suggesting that genomic disruption due to transgenic integration may contribute to the arrest of T cell development. Here we report the identification of the transgenic integration site in tgε26 mice. We found that multiple copies of the human CD3ε transgene are inserted between the Sstr5 and Metrn loci on chromosome 17, and that this is accompanied by duplication of the neighboring genomic region spanning 323 kb. However, none of the genes in this region were abrogated. These results suggest that the severe immunodeficiency seen in tgε26 mice is not due to gene disruption resulting from transgenic integration.http://europepmc.org/articles/PMC3008721?pdf=render |
| spellingShingle | Izumi Ohigashi Yuki Yamasaki Tsukasa Hirashima Yousuke Takahama Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice. PLoS ONE |
| title | Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice. |
| title_full | Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice. |
| title_fullStr | Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice. |
| title_full_unstemmed | Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice. |
| title_short | Identification of the transgenic integration site in immunodeficient tgε26 human CD3ε transgenic mice. |
| title_sort | identification of the transgenic integration site in immunodeficient tgε26 human cd3ε transgenic mice |
| url | http://europepmc.org/articles/PMC3008721?pdf=render |
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