Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa
<i>Arnica montana</i> is a valuable plant with high demand on the pharmaceutical and cosmetic market due to the presence of helenalin (H) and 11α, 13-dihydrohelenalin (DH) sesquiterpene lactones (SLs), with many applications and anti-inflammatory, anti-tumor, analgesic and other properti...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/11/4379 |
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| author | Agata Parafiniuk Krystyna Kromer Mariusz G. Fleszar Agnieszka Kreitschitz Jerzy Wiśniewski Andrzej Gamian |
| author_facet | Agata Parafiniuk Krystyna Kromer Mariusz G. Fleszar Agnieszka Kreitschitz Jerzy Wiśniewski Andrzej Gamian |
| author_sort | Agata Parafiniuk |
| collection | DOAJ |
| description | <i>Arnica montana</i> is a valuable plant with high demand on the pharmaceutical and cosmetic market due to the presence of helenalin (H) and 11α, 13-dihydrohelenalin (DH) sesquiterpene lactones (SLs), with many applications and anti-inflammatory, anti-tumor, analgesic and other properties. Despite the great importance of these compounds for the protection of the plant and their medicinal value, the content of these lactones and the profile of the compounds present within individual elements of florets and flower heads have not been studied so far, and attempts to localize these compounds in flower tissues have also not been conducted. The three studied <i>Arnica</i> taxa synthesize SLs only in the aerial parts of plants, and the highest content of these substances was found in <i>A. montana</i> cv. Arbo; it was lower in wild species, and a very small amount of H was produced by <i>A. chamissonis</i>. Analysis of dissected fragments of whole inflorescences revealed a specific distribution pattern of these compounds. The lactones content in single florets increased from the top of the corolla to the ovary, with the pappus calyx being a significant source of their production. Histochemical tests for terpenes and methylene ketones indicated the colocalization of lactones with inulin vacuoles. |
| first_indexed | 2024-03-11T03:01:34Z |
| format | Article |
| id | doaj.art-57db674b1cd440aba451a0a095a333e7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:01:34Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-57db674b1cd440aba451a0a095a333e72023-11-18T08:15:51ZengMDPI AGMolecules1420-30492023-05-012811437910.3390/molecules28114379Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> TaxaAgata Parafiniuk0Krystyna Kromer1Mariusz G. Fleszar2Agnieszka Kreitschitz3Jerzy Wiśniewski4Andrzej Gamian5Laboratory of Tissue Cultures, Botanical Garden, Faculty of Biological Sciences, University of Wroclaw, Sienkiewicza 23, 50-525 Wroclaw, PolandLaboratory of Tissue Cultures, Botanical Garden, Faculty of Biological Sciences, University of Wroclaw, Sienkiewicza 23, 50-525 Wroclaw, PolandDepartment of Biochemistry and Immunochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, PolandDepartment of Plant Development Biology, Faculty of Biological Sciences, University of Wroclaw, ul. Kanonia 6/8, 50-328 Wroclaw, PolandDepartment of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, 50-370 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland<i>Arnica montana</i> is a valuable plant with high demand on the pharmaceutical and cosmetic market due to the presence of helenalin (H) and 11α, 13-dihydrohelenalin (DH) sesquiterpene lactones (SLs), with many applications and anti-inflammatory, anti-tumor, analgesic and other properties. Despite the great importance of these compounds for the protection of the plant and their medicinal value, the content of these lactones and the profile of the compounds present within individual elements of florets and flower heads have not been studied so far, and attempts to localize these compounds in flower tissues have also not been conducted. The three studied <i>Arnica</i> taxa synthesize SLs only in the aerial parts of plants, and the highest content of these substances was found in <i>A. montana</i> cv. Arbo; it was lower in wild species, and a very small amount of H was produced by <i>A. chamissonis</i>. Analysis of dissected fragments of whole inflorescences revealed a specific distribution pattern of these compounds. The lactones content in single florets increased from the top of the corolla to the ovary, with the pappus calyx being a significant source of their production. Histochemical tests for terpenes and methylene ketones indicated the colocalization of lactones with inulin vacuoles.https://www.mdpi.com/1420-3049/28/11/4379<i>Arnica montana</i><i>A. chamissonis</i>sesquiterpene lactoneshelenalin and dihydrohelenalin estersbiosynthesis localizationhistochemical study |
| spellingShingle | Agata Parafiniuk Krystyna Kromer Mariusz G. Fleszar Agnieszka Kreitschitz Jerzy Wiśniewski Andrzej Gamian Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa Molecules <i>Arnica montana</i> <i>A. chamissonis</i> sesquiterpene lactones helenalin and dihydrohelenalin esters biosynthesis localization histochemical study |
| title | Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa |
| title_full | Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa |
| title_fullStr | Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa |
| title_full_unstemmed | Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa |
| title_short | Localization of Sesquiterpene Lactones Biosynthesis in Flowers of <i>Arnica</i> Taxa |
| title_sort | localization of sesquiterpene lactones biosynthesis in flowers of i arnica i taxa |
| topic | <i>Arnica montana</i> <i>A. chamissonis</i> sesquiterpene lactones helenalin and dihydrohelenalin esters biosynthesis localization histochemical study |
| url | https://www.mdpi.com/1420-3049/28/11/4379 |
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