Diversity and functional characteristics of intestinal microbiome in patients with varicocele
Objective To investigate the changes of intestinal microbiome in patients with varicocele (VC) with macrogenomics. Methods Fresh feces from 15 VC patients and 15 healthy individuals were collected, and DNA sequences were extracted from the feces. The diversity and functional characteristics were an...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Army Medical University
2023-03-01
|
| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/html/202212019.htm |
| _version_ | 1797854125998735360 |
|---|---|
| author | PENG Yisong LIU Shuang TANG Li XIAO Xu TANG Bo |
| author_facet | PENG Yisong LIU Shuang TANG Li XIAO Xu TANG Bo |
| author_sort | PENG Yisong |
| collection | DOAJ |
| description | Objective To investigate the changes of intestinal microbiome in patients with varicocele (VC) with macrogenomics. Methods Fresh feces from 15 VC patients and 15 healthy individuals were collected, and DNA sequences were extracted from the feces. The diversity and functional characteristics were analyzed by metagenome sequencing methods. Results There were no statistical differences in age and body mass index (BMI) between the 2 groups (P > 0.05). The Alpha diversity results showed no significant difference was observed between them (P > 0.05). The Beta diversity results indicated that the species diversity had significant differences in the feces between the VC patients and healthy individuals (P < 0.01). The dominant floras in both the VC patients and healthy individuals were mainly Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria. At species level, the dominant floras in VC patients included Flavonifractor plautii, Methanobrevibacter smithii, Enterobacter roggenkampii, etc, and those in the healthy individuals were Phascolarctobacterium faecium, Romboutsia ilealis, Bifidobacterium bifidum, Prevotella jejuni, Clostridium perfringens, etc. Metagenomics data identified significant up-regulation of D-glucarate degradation pathways, including D-galactarate degradation I, superpathway of D-glucarate and D-galactarate degradation, D-glucarate degradation I, etc. The alterations were associated with intestinal microbiomes Enterococcus faecalis, Flavonifractor plautii, Raoultella terrigena, etc. Conclusion There are obvious characteristics changes in microflora structure between VC patients and healthy controls. Up-regulation of D-glucarate degradation pathways are found in VC patients. These changes of intestinal microbiome might be one of the underlying pathophysiological mechanism of VC.
|
| first_indexed | 2024-04-09T20:01:56Z |
| format | Article |
| id | doaj.art-57e081cd8731442c8b2c9f2e582d48a3 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-09T20:01:56Z |
| publishDate | 2023-03-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-57e081cd8731442c8b2c9f2e582d48a32023-04-03T04:19:30ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-03-0145649349910.16016/j.2097-0927.202212019Diversity and functional characteristics of intestinal microbiome in patients with varicocelePENG Yisong0 LIU Shuang1TANG Li2XIAO Xu3TANG Bo4Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China Objective To investigate the changes of intestinal microbiome in patients with varicocele (VC) with macrogenomics. Methods Fresh feces from 15 VC patients and 15 healthy individuals were collected, and DNA sequences were extracted from the feces. The diversity and functional characteristics were analyzed by metagenome sequencing methods. Results There were no statistical differences in age and body mass index (BMI) between the 2 groups (P > 0.05). The Alpha diversity results showed no significant difference was observed between them (P > 0.05). The Beta diversity results indicated that the species diversity had significant differences in the feces between the VC patients and healthy individuals (P < 0.01). The dominant floras in both the VC patients and healthy individuals were mainly Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria. At species level, the dominant floras in VC patients included Flavonifractor plautii, Methanobrevibacter smithii, Enterobacter roggenkampii, etc, and those in the healthy individuals were Phascolarctobacterium faecium, Romboutsia ilealis, Bifidobacterium bifidum, Prevotella jejuni, Clostridium perfringens, etc. Metagenomics data identified significant up-regulation of D-glucarate degradation pathways, including D-galactarate degradation I, superpathway of D-glucarate and D-galactarate degradation, D-glucarate degradation I, etc. The alterations were associated with intestinal microbiomes Enterococcus faecalis, Flavonifractor plautii, Raoultella terrigena, etc. Conclusion There are obvious characteristics changes in microflora structure between VC patients and healthy controls. Up-regulation of D-glucarate degradation pathways are found in VC patients. These changes of intestinal microbiome might be one of the underlying pathophysiological mechanism of VC. http://aammt.tmmu.edu.cn/html/202212019.htmvaricocelemicrobiomemetagenomics |
| spellingShingle | PENG Yisong LIU Shuang TANG Li XIAO Xu TANG Bo Diversity and functional characteristics of intestinal microbiome in patients with varicocele 陆军军医大学学报 varicocele microbiome metagenomics |
| title | Diversity and functional characteristics of intestinal microbiome in patients with varicocele |
| title_full | Diversity and functional characteristics of intestinal microbiome in patients with varicocele |
| title_fullStr | Diversity and functional characteristics of intestinal microbiome in patients with varicocele |
| title_full_unstemmed | Diversity and functional characteristics of intestinal microbiome in patients with varicocele |
| title_short | Diversity and functional characteristics of intestinal microbiome in patients with varicocele |
| title_sort | diversity and functional characteristics of intestinal microbiome in patients with varicocele |
| topic | varicocele microbiome metagenomics |
| url | http://aammt.tmmu.edu.cn/html/202212019.htm |
| work_keys_str_mv | AT pengyisong diversityandfunctionalcharacteristicsofintestinalmicrobiomeinpatientswithvaricocele AT liushuang diversityandfunctionalcharacteristicsofintestinalmicrobiomeinpatientswithvaricocele AT tangli diversityandfunctionalcharacteristicsofintestinalmicrobiomeinpatientswithvaricocele AT xiaoxu diversityandfunctionalcharacteristicsofintestinalmicrobiomeinpatientswithvaricocele AT tangbo diversityandfunctionalcharacteristicsofintestinalmicrobiomeinpatientswithvaricocele |