Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis
The successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.855715/full |
| _version_ | 1818015371835211776 |
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| author | Eleni Theodosiou Adrian Tüllinghoff Jörg Toepel Bruno Bühler |
| author_facet | Eleni Theodosiou Adrian Tüllinghoff Jörg Toepel Bruno Bühler |
| author_sort | Eleni Theodosiou |
| collection | DOAJ |
| description | The successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or (immobilized) formulations thereof can serve as biocatalysts. Living cells feature, beside active enzymes, metabolic modules that can be exploited to support energy-dependent and multi-step enzyme-catalyzed reactions. Metabolism can sustainably supply necessary cofactors or cosubstrates at the expense of readily available and cheap resources, rendering external addition of costly cosubstrates unnecessary. However, for the development of an efficient whole-cell biocatalyst, in depth comprehension of metabolic modules and their interconnection with cell growth, maintenance, and product formation is indispensable. In order to maximize the flux through biosynthetic reactions and pathways to an industrially relevant product and respective key performance indices (i.e., titer, yield, and productivity), existing metabolic modules can be redesigned and/or novel artificial ones established. This review focuses on whole-cell bioconversions that are coupled to heterotrophic or phototrophic metabolism and discusses metabolic engineering efforts aiming at 1) increasing regeneration and supply of redox equivalents, such as NAD(P/H), 2) blocking competing fluxes, and 3) increasing the availability of metabolites serving as (co)substrates of desired biosynthetic routes. |
| first_indexed | 2024-04-14T06:56:08Z |
| format | Article |
| id | doaj.art-57e80b3da55b471fbbfab2f62031e311 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-14T06:56:08Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-57e80b3da55b471fbbfab2f62031e3112022-12-22T02:06:53ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-04-011010.3389/fbioe.2022.855715855715Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell BiocatalysisEleni Theodosiou0Adrian Tüllinghoff1Jörg Toepel2Bruno Bühler3Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, GreeceDepartment of Solar Materials, Helmholtz Centre for Environmental Research GmbH—UFZ, Leipzig, GermanyDepartment of Solar Materials, Helmholtz Centre for Environmental Research GmbH—UFZ, Leipzig, GermanyDepartment of Solar Materials, Helmholtz Centre for Environmental Research GmbH—UFZ, Leipzig, GermanyThe successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or (immobilized) formulations thereof can serve as biocatalysts. Living cells feature, beside active enzymes, metabolic modules that can be exploited to support energy-dependent and multi-step enzyme-catalyzed reactions. Metabolism can sustainably supply necessary cofactors or cosubstrates at the expense of readily available and cheap resources, rendering external addition of costly cosubstrates unnecessary. However, for the development of an efficient whole-cell biocatalyst, in depth comprehension of metabolic modules and their interconnection with cell growth, maintenance, and product formation is indispensable. In order to maximize the flux through biosynthetic reactions and pathways to an industrially relevant product and respective key performance indices (i.e., titer, yield, and productivity), existing metabolic modules can be redesigned and/or novel artificial ones established. This review focuses on whole-cell bioconversions that are coupled to heterotrophic or phototrophic metabolism and discusses metabolic engineering efforts aiming at 1) increasing regeneration and supply of redox equivalents, such as NAD(P/H), 2) blocking competing fluxes, and 3) increasing the availability of metabolites serving as (co)substrates of desired biosynthetic routes.https://www.frontiersin.org/articles/10.3389/fbioe.2022.855715/fullwhole-cell redox biocatalysiscentral metabolismTCA cyclemetabolic engineeringcyanobacteriaredox balance |
| spellingShingle | Eleni Theodosiou Adrian Tüllinghoff Jörg Toepel Bruno Bühler Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis Frontiers in Bioengineering and Biotechnology whole-cell redox biocatalysis central metabolism TCA cycle metabolic engineering cyanobacteria redox balance |
| title | Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis |
| title_full | Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis |
| title_fullStr | Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis |
| title_full_unstemmed | Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis |
| title_short | Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis |
| title_sort | exploitation of hetero and phototrophic metabolic modules for redox intensive whole cell biocatalysis |
| topic | whole-cell redox biocatalysis central metabolism TCA cycle metabolic engineering cyanobacteria redox balance |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.855715/full |
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