Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a serious infection-causing pathogen in humans and animal. In particular, methicillin-resistant <i>S. aureus</i> (MRSA) is considered one of the major life-threatening pathogens due to its rapid resistance to seve...
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MDPI AG
2023-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/205 |
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| author | Hanan A. Zaher Shimaa El Baz Abdulaziz S. Alothaim Sulaiman A. Alsalamah Mohammed Ibrahim Alghonaim Abdullah S. Alawam Mostafa M. Eraqi |
| author_facet | Hanan A. Zaher Shimaa El Baz Abdulaziz S. Alothaim Sulaiman A. Alsalamah Mohammed Ibrahim Alghonaim Abdullah S. Alawam Mostafa M. Eraqi |
| author_sort | Hanan A. Zaher |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a serious infection-causing pathogen in humans and animal. In particular, methicillin-resistant <i>S. aureus</i> (MRSA) is considered one of the major life-threatening pathogens due to its rapid resistance to several antibiotics in clinical practice. MRSA strains have recently been isolated in a number of animals utilized in food production processes, and these species are thought to be the important sources of the spread of infection and disease in both humans and animals. The main objective of the current study was to assess the prevalence of drug-resistant <i>S. aureus</i>, particularly vancomycin-resistant <i>S. aureus</i> (VRSA) and MRSA, by molecular methods. To address this issue, a total of three hundred samples (200 meat samples from cattle and sheep carcasses (100 of each), 50 hand swabs, and 50 stool samples from abattoir workers) were obtained from slaughterhouses in Egypt provinces. In total, 19% <i>S. aureus</i> was isolated by standard culture techniques, and the antibiotic resistance was confirmed genotypically by amplification <i>nuc</i>A gen. Characteristic resistance genes were identified by PCR with incidence of 31.5%, 19.3%, 8.7%, and 7% for the <i>mec</i>A, <i>Van</i>A, <i>erm</i>A, and <i>tet</i> L genes, respectively, while the <i>aac6-aph</i> gene was not found in any of the isolates. In this study, the virulence genes responsible for <i>S. aureus’</i> resistance to antibiotics had the highest potential for infection or disease transmission to animal carcasses, slaughterhouse workers, and meat products. |
| first_indexed | 2024-03-11T09:15:57Z |
| format | Article |
| id | doaj.art-57ed296a59774af68eb2d84df2bb3b3d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:15:57Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-57ed296a59774af68eb2d84df2bb3b3d2023-11-16T18:41:39ZengMDPI AGAntibiotics2079-63822023-01-0112220510.3390/antibiotics12020205Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse WorkersHanan A. Zaher0Shimaa El Baz1Abdulaziz S. Alothaim2Sulaiman A. Alsalamah3Mohammed Ibrahim Alghonaim4Abdullah S. Alawam5Mostafa M. Eraqi6Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi ArabiaDepartment of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi ArabiaDepartment of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi ArabiaDepartment of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi ArabiaDepartment of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a serious infection-causing pathogen in humans and animal. In particular, methicillin-resistant <i>S. aureus</i> (MRSA) is considered one of the major life-threatening pathogens due to its rapid resistance to several antibiotics in clinical practice. MRSA strains have recently been isolated in a number of animals utilized in food production processes, and these species are thought to be the important sources of the spread of infection and disease in both humans and animals. The main objective of the current study was to assess the prevalence of drug-resistant <i>S. aureus</i>, particularly vancomycin-resistant <i>S. aureus</i> (VRSA) and MRSA, by molecular methods. To address this issue, a total of three hundred samples (200 meat samples from cattle and sheep carcasses (100 of each), 50 hand swabs, and 50 stool samples from abattoir workers) were obtained from slaughterhouses in Egypt provinces. In total, 19% <i>S. aureus</i> was isolated by standard culture techniques, and the antibiotic resistance was confirmed genotypically by amplification <i>nuc</i>A gen. Characteristic resistance genes were identified by PCR with incidence of 31.5%, 19.3%, 8.7%, and 7% for the <i>mec</i>A, <i>Van</i>A, <i>erm</i>A, and <i>tet</i> L genes, respectively, while the <i>aac6-aph</i> gene was not found in any of the isolates. In this study, the virulence genes responsible for <i>S. aureus’</i> resistance to antibiotics had the highest potential for infection or disease transmission to animal carcasses, slaughterhouse workers, and meat products.https://www.mdpi.com/2079-6382/12/2/205<i>Staphylococcus aureus</i>MRSAVRSAcattlesheep |
| spellingShingle | Hanan A. Zaher Shimaa El Baz Abdulaziz S. Alothaim Sulaiman A. Alsalamah Mohammed Ibrahim Alghonaim Abdullah S. Alawam Mostafa M. Eraqi Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers Antibiotics <i>Staphylococcus aureus</i> MRSA VRSA cattle sheep |
| title | Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers |
| title_full | Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers |
| title_fullStr | Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers |
| title_full_unstemmed | Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers |
| title_short | Molecular Basis of Methicillin and Vancomycin Resistance in <i>Staphylococcus aureus</i> from Cattle, Sheep Carcasses and Slaughterhouse Workers |
| title_sort | molecular basis of methicillin and vancomycin resistance in i staphylococcus aureus i from cattle sheep carcasses and slaughterhouse workers |
| topic | <i>Staphylococcus aureus</i> MRSA VRSA cattle sheep |
| url | https://www.mdpi.com/2079-6382/12/2/205 |
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