Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria

Background and Aim: Green tea, a rich source of flavonoids, has tremendous oral health potential. This study evaluates the effect of brewing green tea at varying concentrations and duration on its antimicrobial activity against oral bacteria. Materials and Methods: Green tea was brewed in 15 concent...

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Main Authors: Harpreet Singh Grover, Himanshu Deswal, Veenu Madaan Hans, L Sumitra Devi
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2017-01-01
Series:Saudi Journal of Oral Sciences
Subjects:
Online Access:http://www.saudijos.org//article.asp?issn=1658-6816;year=2017;volume=4;issue=1;spage=18;epage=21;aulast=Grover
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author Harpreet Singh Grover
Himanshu Deswal
Veenu Madaan Hans
L Sumitra Devi
author_facet Harpreet Singh Grover
Himanshu Deswal
Veenu Madaan Hans
L Sumitra Devi
author_sort Harpreet Singh Grover
collection DOAJ
description Background and Aim: Green tea, a rich source of flavonoids, has tremendous oral health potential. This study evaluates the effect of brewing green tea at varying concentrations and duration on its antimicrobial activity against oral bacteria. Materials and Methods: Green tea was brewed in 15 concentration and time combinations. Paper disc diffusion test was carried out with these infusions on Tryptic Soy Agar plates inoculated with oral bacteria. Zone of inhibition after 72 h of incubation at 37°C was measured in millimeters. Results: Higher zone of inhibition was observed in concentration brewed for 15 min than 30 and 60 min. Zone of inhibition was directly proportional to the concentration of green tea brewed. Most effective concentration was 80 mg/ml with a mean of 2.83 mm which was significantly higher than mean zone of inhibition for 60 mg/ml (P < 0.05). Conclusion: Increasing the concentration and decreasing the brewing time of green tea results in a higher inhibitory effect on growth of oral bacteria.
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spelling doaj.art-57efb9ed1a7843d49d020dbe4dc349e32022-12-22T03:35:16ZengWolters Kluwer Medknow PublicationsSaudi Journal of Oral Sciences1658-68162017-01-0141182110.4103/1658-6816.200138Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteriaHarpreet Singh GroverHimanshu DeswalVeenu Madaan HansL Sumitra DeviBackground and Aim: Green tea, a rich source of flavonoids, has tremendous oral health potential. This study evaluates the effect of brewing green tea at varying concentrations and duration on its antimicrobial activity against oral bacteria. Materials and Methods: Green tea was brewed in 15 concentration and time combinations. Paper disc diffusion test was carried out with these infusions on Tryptic Soy Agar plates inoculated with oral bacteria. Zone of inhibition after 72 h of incubation at 37°C was measured in millimeters. Results: Higher zone of inhibition was observed in concentration brewed for 15 min than 30 and 60 min. Zone of inhibition was directly proportional to the concentration of green tea brewed. Most effective concentration was 80 mg/ml with a mean of 2.83 mm which was significantly higher than mean zone of inhibition for 60 mg/ml (P < 0.05). Conclusion: Increasing the concentration and decreasing the brewing time of green tea results in a higher inhibitory effect on growth of oral bacteria.http://www.saudijos.org//article.asp?issn=1658-6816;year=2017;volume=4;issue=1;spage=18;epage=21;aulast=GroverAntibacterialCamellia sinensisgreen teaoral health
spellingShingle Harpreet Singh Grover
Himanshu Deswal
Veenu Madaan Hans
L Sumitra Devi
Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria
Saudi Journal of Oral Sciences
Antibacterial
Camellia sinensis
green tea
oral health
title Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria
title_full Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria
title_fullStr Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria
title_full_unstemmed Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria
title_short Effect of concentration and brewing time on the antimicrobial activity of Camellia sinensis (green tea) against oral bacteria
title_sort effect of concentration and brewing time on the antimicrobial activity of camellia sinensis green tea against oral bacteria
topic Antibacterial
Camellia sinensis
green tea
oral health
url http://www.saudijos.org//article.asp?issn=1658-6816;year=2017;volume=4;issue=1;spage=18;epage=21;aulast=Grover
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