The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs
BackgroundEndolymphatic hydrops (EH) is considered as the pathological correlate of Menière’s disease (MD) and cause of hearing loss. The mechanism of EH, remaining unrevealed, poses challenges for formalized clinical trials.ObjectiveThis study aims to investigate the development of hearing loss, as...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2022.836093/full |
| _version_ | 1819023328537477120 |
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| author | Shu-Qi Wang Shu-Qi Wang Chen-Long Li Chen-Long Li Chen-Long Li Jing-Qi Xu Jing-Qi Xu Li-Li Chen Li-Li Chen Li-Li Chen You-Zhou Xie You-Zhou Xie You-Zhou Xie Pei-Dong Dai Pei-Dong Dai Liu-Jie Ren Liu-Jie Ren Liu-Jie Ren Wen-Juan Yao Wen-Juan Yao Tian-Yu Zhang Tian-Yu Zhang Tian-Yu Zhang |
| author_facet | Shu-Qi Wang Shu-Qi Wang Chen-Long Li Chen-Long Li Chen-Long Li Jing-Qi Xu Jing-Qi Xu Li-Li Chen Li-Li Chen Li-Li Chen You-Zhou Xie You-Zhou Xie You-Zhou Xie Pei-Dong Dai Pei-Dong Dai Liu-Jie Ren Liu-Jie Ren Liu-Jie Ren Wen-Juan Yao Wen-Juan Yao Tian-Yu Zhang Tian-Yu Zhang Tian-Yu Zhang |
| author_sort | Shu-Qi Wang |
| collection | DOAJ |
| description | BackgroundEndolymphatic hydrops (EH) is considered as the pathological correlate of Menière’s disease (MD) and cause of hearing loss. The mechanism of EH, remaining unrevealed, poses challenges for formalized clinical trials.ObjectiveThis study aims to investigate the development of hearing loss, as well as the effect of dehydration treatment on EH animal models.MethodsIn this study, different severity EH animal models were created. The laser Doppler vibrometer (LDV) and auditory brainstem responses (ABR) were used to study the effects of EH and the dehydration effects of mannitol. The LDV was used to measure the vibration of the round window membrane (RWM) reflecting the changes in inner ear impedance. ABR was used to evaluate the hearing changes. Furthermore, tissue section and scanning electron microscopy (SEM) observations were used to analyze the anatomical change to the cochlea and outer hair cells.ResultsThe RWM vibrations decreased with the severity of EH, indicating an increase in the cochlear impedance. The dehydration therapy lowered the impedance to restore acoustic transduction in EH 10- and 20-day animal models. Simultaneously, the ABR thresholds increased in EH models and were restored after dehydration. Moreover, a difference in the hearing was found between ABR and LDV results in severe EH animal models, and the dehydration therapy was less effective, indicating a sensorineural hearing loss (SNHL).ConclusionEndolymphatic hydrops causes hearing loss by increasing the cochlear impedance in all tested groups, and mannitol dehydration is an effective therapy to restore hearing. However, SNHL occurs for the EH 30-day animal models, limiting the effectiveness of dehydration. Our results suggest the use of dehydrating agents in the early stage of EH. |
| first_indexed | 2024-12-21T04:37:09Z |
| format | Article |
| id | doaj.art-57f1000913334faea985f5ba6c7faff9 |
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| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-21T04:37:09Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-57f1000913334faea985f5ba6c7faff92022-12-21T19:15:49ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-04-011610.3389/fncel.2022.836093836093The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea PigsShu-Qi Wang0Shu-Qi Wang1Chen-Long Li2Chen-Long Li3Chen-Long Li4Jing-Qi Xu5Jing-Qi Xu6Li-Li Chen7Li-Li Chen8Li-Li Chen9You-Zhou Xie10You-Zhou Xie11You-Zhou Xie12Pei-Dong Dai13Pei-Dong Dai14Liu-Jie Ren15Liu-Jie Ren16Liu-Jie Ren17Wen-Juan Yao18Wen-Juan Yao19Tian-Yu Zhang20Tian-Yu Zhang21Tian-Yu Zhang22Department of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaHearing Medicine Key Laboratory, National Health Commission of China, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaHearing Medicine Key Laboratory, National Health Commission of China, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaHearing Medicine Key Laboratory, National Health Commission of China, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaHearing Medicine Key Laboratory, National Health Commission of China, Shanghai, ChinaSchool of Mechanics and Engineering Science, Shanghai University, Shanghai, ChinaShanghai Institute of Applied Mathematics and Mechanics, Shanghai, ChinaDepartment of Facial Plastic Reconstruction Surgery, Eye and ENT Hospital of Fudan University, Shanghai, ChinaENT Institute, Eye and ENT Hospital of Fudan University, Shanghai, ChinaHearing Medicine Key Laboratory, National Health Commission of China, Shanghai, ChinaBackgroundEndolymphatic hydrops (EH) is considered as the pathological correlate of Menière’s disease (MD) and cause of hearing loss. The mechanism of EH, remaining unrevealed, poses challenges for formalized clinical trials.ObjectiveThis study aims to investigate the development of hearing loss, as well as the effect of dehydration treatment on EH animal models.MethodsIn this study, different severity EH animal models were created. The laser Doppler vibrometer (LDV) and auditory brainstem responses (ABR) were used to study the effects of EH and the dehydration effects of mannitol. The LDV was used to measure the vibration of the round window membrane (RWM) reflecting the changes in inner ear impedance. ABR was used to evaluate the hearing changes. Furthermore, tissue section and scanning electron microscopy (SEM) observations were used to analyze the anatomical change to the cochlea and outer hair cells.ResultsThe RWM vibrations decreased with the severity of EH, indicating an increase in the cochlear impedance. The dehydration therapy lowered the impedance to restore acoustic transduction in EH 10- and 20-day animal models. Simultaneously, the ABR thresholds increased in EH models and were restored after dehydration. Moreover, a difference in the hearing was found between ABR and LDV results in severe EH animal models, and the dehydration therapy was less effective, indicating a sensorineural hearing loss (SNHL).ConclusionEndolymphatic hydrops causes hearing loss by increasing the cochlear impedance in all tested groups, and mannitol dehydration is an effective therapy to restore hearing. However, SNHL occurs for the EH 30-day animal models, limiting the effectiveness of dehydration. Our results suggest the use of dehydrating agents in the early stage of EH.https://www.frontiersin.org/articles/10.3389/fncel.2022.836093/fullendolymphatic hydropsdehydration therapyMénière’s diseaselaser Doppler vibrometrycochlear impedance |
| spellingShingle | Shu-Qi Wang Shu-Qi Wang Chen-Long Li Chen-Long Li Chen-Long Li Jing-Qi Xu Jing-Qi Xu Li-Li Chen Li-Li Chen Li-Li Chen You-Zhou Xie You-Zhou Xie You-Zhou Xie Pei-Dong Dai Pei-Dong Dai Liu-Jie Ren Liu-Jie Ren Liu-Jie Ren Wen-Juan Yao Wen-Juan Yao Tian-Yu Zhang Tian-Yu Zhang Tian-Yu Zhang The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs Frontiers in Cellular Neuroscience endolymphatic hydrops dehydration therapy Ménière’s disease laser Doppler vibrometry cochlear impedance |
| title | The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs |
| title_full | The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs |
| title_fullStr | The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs |
| title_full_unstemmed | The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs |
| title_short | The Effect of Endolymphatic Hydrops and Mannitol Dehydration Treatment on Guinea Pigs |
| title_sort | effect of endolymphatic hydrops and mannitol dehydration treatment on guinea pigs |
| topic | endolymphatic hydrops dehydration therapy Ménière’s disease laser Doppler vibrometry cochlear impedance |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2022.836093/full |
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