Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis
Abstract Background Huazhuo Tiaozhi granule (HTG) is a herbal medicine formula widely used in clinical practice for hypolipidaemic effects. However, the molecular mechanisms underlying dyslipidaemia treatment have not been well elucidated. Results A significant reduction in the levels of total chole...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Clinical Epigenetics |
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| Online Access: | https://doi.org/10.1186/s13148-023-01573-y |
| _version_ | 1797636817184358400 |
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| author | Xiangjun Yin Min Li Yongzhi Wang Guifang Zhao Tao Yang Yuqing Zhang Jianbo Guo Tiantian Meng Ruolin Du Honglin Li Zhe Wang Jian Zhang Qingyong He |
| author_facet | Xiangjun Yin Min Li Yongzhi Wang Guifang Zhao Tao Yang Yuqing Zhang Jianbo Guo Tiantian Meng Ruolin Du Honglin Li Zhe Wang Jian Zhang Qingyong He |
| author_sort | Xiangjun Yin |
| collection | DOAJ |
| description | Abstract Background Huazhuo Tiaozhi granule (HTG) is a herbal medicine formula widely used in clinical practice for hypolipidaemic effects. However, the molecular mechanisms underlying dyslipidaemia treatment have not been well elucidated. Results A significant reduction in the levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) was observed in the serum of patients with dyslipidaemia after HTG treatment, without disruption in the levels of aspartate transaminase (AST), alanine transaminase (ALT), urea nitrogen (BUN), and creatinine (Cr). The dyslipidaemia rat model was induced by a high-fat diet and treated with Xuezhikang (0.14 g/kg/d) or HTG (9.33 g crude herb/kg/day) by gavage for 8 weeks. Body weight and liver index were markedly decreased in dyslipidaemic rats after treatment with Xuezhikang or HTG. HTG administration markedly ameliorated hyperlipidaemia by decreasing the levels of TC and LDL-C in serum and hepatic lipid accumulation. In vitro, lipid accumulation in LO2 and HepG2 cells was alleviated by serum treatment with HTG. High lactylation was observed in 198 proteins, including lactylation of histone H2B (K6), H4 (K80). Deep sequencing of microRNAs showed that miR-155-5p was significantly downregulated. Conclusions This study demonstrates that HTG is an effective and safe formula for treating dyslipidaemia, which promotes lactylation in hepatocytes, and the retardation of miR-155-5p biogenesis. Graphical abstract |
| first_indexed | 2024-03-11T12:39:35Z |
| format | Article |
| id | doaj.art-57f2bd54839f4951b75433724fb89436 |
| institution | Directory Open Access Journal |
| issn | 1868-7083 |
| language | English |
| last_indexed | 2024-03-11T12:39:35Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Epigenetics |
| spelling | doaj.art-57f2bd54839f4951b75433724fb894362023-11-05T12:21:40ZengBMCClinical Epigenetics1868-70832023-11-0115111410.1186/s13148-023-01573-yHerbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesisXiangjun Yin0Min Li1Yongzhi Wang2Guifang Zhao3Tao Yang4Yuqing Zhang5Jianbo Guo6Tiantian Meng7Ruolin Du8Honglin Li9Zhe Wang10Jian Zhang11Qingyong He12School of Basic Medical Science, Zhejiang Chinese Medical UniversityDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesShanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and TechnologyDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesLKS Faculty of Medicine, The University of Hong KongDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesShanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and TechnologyDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesShanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and TechnologyDepartment of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical SciencesAbstract Background Huazhuo Tiaozhi granule (HTG) is a herbal medicine formula widely used in clinical practice for hypolipidaemic effects. However, the molecular mechanisms underlying dyslipidaemia treatment have not been well elucidated. Results A significant reduction in the levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) was observed in the serum of patients with dyslipidaemia after HTG treatment, without disruption in the levels of aspartate transaminase (AST), alanine transaminase (ALT), urea nitrogen (BUN), and creatinine (Cr). The dyslipidaemia rat model was induced by a high-fat diet and treated with Xuezhikang (0.14 g/kg/d) or HTG (9.33 g crude herb/kg/day) by gavage for 8 weeks. Body weight and liver index were markedly decreased in dyslipidaemic rats after treatment with Xuezhikang or HTG. HTG administration markedly ameliorated hyperlipidaemia by decreasing the levels of TC and LDL-C in serum and hepatic lipid accumulation. In vitro, lipid accumulation in LO2 and HepG2 cells was alleviated by serum treatment with HTG. High lactylation was observed in 198 proteins, including lactylation of histone H2B (K6), H4 (K80). Deep sequencing of microRNAs showed that miR-155-5p was significantly downregulated. Conclusions This study demonstrates that HTG is an effective and safe formula for treating dyslipidaemia, which promotes lactylation in hepatocytes, and the retardation of miR-155-5p biogenesis. Graphical abstracthttps://doi.org/10.1186/s13148-023-01573-yDyslipidaemiaLactylationHistonemiR-155-5pHerbal medicine formulaHuazhuo Tiaozhi granule |
| spellingShingle | Xiangjun Yin Min Li Yongzhi Wang Guifang Zhao Tao Yang Yuqing Zhang Jianbo Guo Tiantian Meng Ruolin Du Honglin Li Zhe Wang Jian Zhang Qingyong He Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis Clinical Epigenetics Dyslipidaemia Lactylation Histone miR-155-5p Herbal medicine formula Huazhuo Tiaozhi granule |
| title | Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis |
| title_full | Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis |
| title_fullStr | Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis |
| title_full_unstemmed | Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis |
| title_short | Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis |
| title_sort | herbal medicine formula huazhuo tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and mir 155 5p biogenesis |
| topic | Dyslipidaemia Lactylation Histone miR-155-5p Herbal medicine formula Huazhuo Tiaozhi granule |
| url | https://doi.org/10.1186/s13148-023-01573-y |
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