TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals
<i>Streptococcus suis</i> (<i>S. suis</i>) serotype 2 (SS2) is the causative agent of swine streptococcosis and can cause severe diseases in both pigs and humans. Although the traditional inactive vaccine can protect pigs from SS2 infection, novel vaccine candidates are neede...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/2/184 |
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| author | Zhaofei Wang Mengting Guo Licheng Kong Ya Gao Jingjiao Ma Yuqiang Cheng Henan Wang Yaxian Yan Jianhe Sun |
| author_facet | Zhaofei Wang Mengting Guo Licheng Kong Ya Gao Jingjiao Ma Yuqiang Cheng Henan Wang Yaxian Yan Jianhe Sun |
| author_sort | Zhaofei Wang |
| collection | DOAJ |
| description | <i>Streptococcus suis</i> (<i>S. suis</i>) serotype 2 (SS2) is the causative agent of swine streptococcosis and can cause severe diseases in both pigs and humans. Although the traditional inactive vaccine can protect pigs from SS2 infection, novel vaccine candidates are needed to overcome its shortcomings. Three infection-associated proteins in <i>S. suis</i>—muramidase-released protein (MRP), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and DLD, a novel putative dihydrolipoamide dehydrogenase—have been previously identified by immunoproteomic assays. In this study, the effective immune protection of the recombinant trivalent protein GAPDH-MRP-DLD (JointS) against SS2, SS7, and SS9 was determined in zebrafish. To improve the immune efficacy of JointS, monophosphoryl lipid A (MPLA) as a TLR4 agonist adjuvant, which induces a strong innate immune response in the immune cells of mice and pigs, was combined with JointS to immunize the mice. The results showed that immunized mice could induce the production of a high titer of anti-<i>S. suis</i> antibodies; as a result, 100% of mice survived after SS2 infection. Furthermore, JointS provides good protection against virulent SS2 strain infections in piglets. Given the above, there is potential to develop JointS as a novel subunit vaccine for piglets to prevent infection by SS2 and other <i>S. suis</i> serotypes. |
| first_indexed | 2024-03-09T00:38:24Z |
| format | Article |
| id | doaj.art-57f44d2f4bf841cc987d1608d93ec6d8 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T00:38:24Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-57f44d2f4bf841cc987d1608d93ec6d82023-12-11T18:00:22ZengMDPI AGVaccines2076-393X2021-02-019218410.3390/vaccines9020184TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in AnimalsZhaofei Wang0Mengting Guo1Licheng Kong2Ya Gao3Jingjiao Ma4Yuqiang Cheng5Henan Wang6Yaxian Yan7Jianhe Sun8Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<i>Streptococcus suis</i> (<i>S. suis</i>) serotype 2 (SS2) is the causative agent of swine streptococcosis and can cause severe diseases in both pigs and humans. Although the traditional inactive vaccine can protect pigs from SS2 infection, novel vaccine candidates are needed to overcome its shortcomings. Three infection-associated proteins in <i>S. suis</i>—muramidase-released protein (MRP), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and DLD, a novel putative dihydrolipoamide dehydrogenase—have been previously identified by immunoproteomic assays. In this study, the effective immune protection of the recombinant trivalent protein GAPDH-MRP-DLD (JointS) against SS2, SS7, and SS9 was determined in zebrafish. To improve the immune efficacy of JointS, monophosphoryl lipid A (MPLA) as a TLR4 agonist adjuvant, which induces a strong innate immune response in the immune cells of mice and pigs, was combined with JointS to immunize the mice. The results showed that immunized mice could induce the production of a high titer of anti-<i>S. suis</i> antibodies; as a result, 100% of mice survived after SS2 infection. Furthermore, JointS provides good protection against virulent SS2 strain infections in piglets. Given the above, there is potential to develop JointS as a novel subunit vaccine for piglets to prevent infection by SS2 and other <i>S. suis</i> serotypes.https://www.mdpi.com/2076-393X/9/2/184<i>Streptococcus suis</i>JointSzebrafishmicepigletsubunit vaccine |
| spellingShingle | Zhaofei Wang Mengting Guo Licheng Kong Ya Gao Jingjiao Ma Yuqiang Cheng Henan Wang Yaxian Yan Jianhe Sun TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals Vaccines <i>Streptococcus suis</i> JointS zebrafish mice piglet subunit vaccine |
| title | TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals |
| title_full | TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals |
| title_fullStr | TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals |
| title_full_unstemmed | TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals |
| title_short | TLR4 Agonist Combined with Trivalent Protein JointS of <i>Streptococcus suis</i> Provides Immunological Protection in Animals |
| title_sort | tlr4 agonist combined with trivalent protein joints of i streptococcus suis i provides immunological protection in animals |
| topic | <i>Streptococcus suis</i> JointS zebrafish mice piglet subunit vaccine |
| url | https://www.mdpi.com/2076-393X/9/2/184 |
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