Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells
ABSTRACT: Background: Glial tumors are the most common primary malignant central nervous system tumors. They are hard to treat, not only because of the deregulation in multiple pathways but also because they are not contained in a well-defined mass with clear borders. The use of a single therapeuti...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Current Therapeutic Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0011393X2300005X |
| _version_ | 1827920704366968832 |
|---|---|
| author | Ashaq Hussain Mir, PhD Mir Khurshid Iqbal, PhD Mujeeb Zafar Banday, PhD Henah Mehraj Balkhi, PhD Ehtishamul Haq, PhD |
| author_facet | Ashaq Hussain Mir, PhD Mir Khurshid Iqbal, PhD Mujeeb Zafar Banday, PhD Henah Mehraj Balkhi, PhD Ehtishamul Haq, PhD |
| author_sort | Ashaq Hussain Mir, PhD |
| collection | DOAJ |
| description | ABSTRACT: Background: Glial tumors are the most common primary malignant central nervous system tumors. They are hard to treat, not only because of the deregulation in multiple pathways but also because they are not contained in a well-defined mass with clear borders. The use of a single therapeutic agent to target gliomas has yielded unsatisfactory results. Objective: A combination of molecules targeting multiple pathways may prove to be a better alternative. Methods: The effect of caffeic acid phenethyl ester and crocin on the proliferation and death of U87-MG cells over a concentration range was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. A colony formation assay was used to measure the effect of caffeic acid phenethyl ester and crocin on contact inhibition and anchorage independence ability of U87-MG cells. Furthermore, apoptosis in U87-MG cells was analyzed by propidium iodide assay. Real-time polymerase chain reaction and Western blotting were performed to determine the expression level of p53, epidermal growth factor receptor, and proliferating cell nuclear antigen. Results: Caffeic acid phenethyl ester and crocin when used in combination present an anticancer potential for glioma. These molecules, in combination, inhibit proliferation and induce apoptosis in U87-MG glioma cells. Our results provide evidence that combination treatment realigns the expression paradigm of p53, epidermal growth factor receptor, and proliferating cell nuclear antigen in cotreated U87-MG cells. Conclusions: The combination of caffeic acid phenethyl ester and crocin led to inhibition in glioma cell proliferation and might prove to be an effective adjunct to the therapies in vogue. |
| first_indexed | 2024-03-13T04:14:35Z |
| format | Article |
| id | doaj.art-57f4536e30ad414592658695e21829ff |
| institution | Directory Open Access Journal |
| issn | 0011-393X |
| language | English |
| last_indexed | 2024-03-13T04:14:35Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Therapeutic Research |
| spelling | doaj.art-57f4536e30ad414592658695e21829ff2023-06-21T06:50:44ZengElsevierCurrent Therapeutic Research0011-393X2023-01-0198100695Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma CellsAshaq Hussain Mir, PhD0Mir Khurshid Iqbal, PhD1Mujeeb Zafar Banday, PhD2Henah Mehraj Balkhi, PhD3Ehtishamul Haq, PhD4Department of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, IndiaDepartment of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, IndiaDepartment of Biochemistry, Government Medical College, University of Kashmir, Srinagar, Jammu and Kashmir, IndiaDepartment of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, IndiaDepartment of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India; Address correspondence to: Ehtishamul Haq, Department of Biotechnology, Ground Floor, Science Block, University of Kashmir, Srinagar - 190006, Kashmir, Jammu and Kashmir, India.ABSTRACT: Background: Glial tumors are the most common primary malignant central nervous system tumors. They are hard to treat, not only because of the deregulation in multiple pathways but also because they are not contained in a well-defined mass with clear borders. The use of a single therapeutic agent to target gliomas has yielded unsatisfactory results. Objective: A combination of molecules targeting multiple pathways may prove to be a better alternative. Methods: The effect of caffeic acid phenethyl ester and crocin on the proliferation and death of U87-MG cells over a concentration range was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. A colony formation assay was used to measure the effect of caffeic acid phenethyl ester and crocin on contact inhibition and anchorage independence ability of U87-MG cells. Furthermore, apoptosis in U87-MG cells was analyzed by propidium iodide assay. Real-time polymerase chain reaction and Western blotting were performed to determine the expression level of p53, epidermal growth factor receptor, and proliferating cell nuclear antigen. Results: Caffeic acid phenethyl ester and crocin when used in combination present an anticancer potential for glioma. These molecules, in combination, inhibit proliferation and induce apoptosis in U87-MG glioma cells. Our results provide evidence that combination treatment realigns the expression paradigm of p53, epidermal growth factor receptor, and proliferating cell nuclear antigen in cotreated U87-MG cells. Conclusions: The combination of caffeic acid phenethyl ester and crocin led to inhibition in glioma cell proliferation and might prove to be an effective adjunct to the therapies in vogue.http://www.sciencedirect.com/science/article/pii/S0011393X2300005XCAPEcrocinCrocus sativusgliomap53U-87MG |
| spellingShingle | Ashaq Hussain Mir, PhD Mir Khurshid Iqbal, PhD Mujeeb Zafar Banday, PhD Henah Mehraj Balkhi, PhD Ehtishamul Haq, PhD Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells Current Therapeutic Research CAPE crocin Crocus sativus glioma p53 U-87MG |
| title | Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells |
| title_full | Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells |
| title_fullStr | Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells |
| title_full_unstemmed | Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells |
| title_short | Combination of Caffeic Acid Phenethyl Ester and Crocin Realign Potential Molecular Markers in U87-MG Glioma Cells |
| title_sort | combination of caffeic acid phenethyl ester and crocin realign potential molecular markers in u87 mg glioma cells |
| topic | CAPE crocin Crocus sativus glioma p53 U-87MG |
| url | http://www.sciencedirect.com/science/article/pii/S0011393X2300005X |
| work_keys_str_mv | AT ashaqhussainmirphd combinationofcaffeicacidphenethylesterandcrocinrealignpotentialmolecularmarkersinu87mggliomacells AT mirkhurshidiqbalphd combinationofcaffeicacidphenethylesterandcrocinrealignpotentialmolecularmarkersinu87mggliomacells AT mujeebzafarbandayphd combinationofcaffeicacidphenethylesterandcrocinrealignpotentialmolecularmarkersinu87mggliomacells AT henahmehrajbalkhiphd combinationofcaffeicacidphenethylesterandcrocinrealignpotentialmolecularmarkersinu87mggliomacells AT ehtishamulhaqphd combinationofcaffeicacidphenethylesterandcrocinrealignpotentialmolecularmarkersinu87mggliomacells |