LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype
Objectives: (1) To investigate the expression patterns of MΦ1 and MΦ2 phenotype markers of peripheral blood monocyte (PBMC)-derived macrophages in atherosclerosis patients and healthy controls, as well as the expression correlation among these genes. (2) To elucidate whether a high level of liver X...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-04-01
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| Series: | Clinical and Experimental Hypertension |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641963.2017.1288740 |
| _version_ | 1797681512337899520 |
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| author | Mengyuan Liu Weijian Yang Shuling Liu Duncan Hock Bohao Zhang Ruth Ya Huo Xiaoguang Tong Hua Yan |
| author_facet | Mengyuan Liu Weijian Yang Shuling Liu Duncan Hock Bohao Zhang Ruth Ya Huo Xiaoguang Tong Hua Yan |
| author_sort | Mengyuan Liu |
| collection | DOAJ |
| description | Objectives: (1) To investigate the expression patterns of MΦ1 and MΦ2 phenotype markers of peripheral blood monocyte (PBMC)-derived macrophages in atherosclerosis patients and healthy controls, as well as the expression correlation among these genes. (2) To elucidate whether a high level of liver X receptor α (LXRα) expression is associated with anti-inflammatory MΦ2-type polarization. Design: Peripheral blood monocytes (PBMCs) were obtained from 28 patients with carotid artery plaques and 10 normal persons, who did not have carotid artery plaques. M1 and M2 phenotype markers were analyzed after cellular differentiation into macrophages. Human macrophages derived from healthy donors were transfected with plasmid DNA encoding LXRα and control null-plasmids. Gene expression levels were quantified after further differentiation. Results: Three genes (LXRα, CD68, and CD36) were expressed at a significantly lower rate in the atherosclerotic group than normal patients. There were correlations between the expression of LXRα, CD68, and peroxisome proliferator-activated receptor (PPARγ), and between CD163, CD36 and scavenger receptor class A (SRA1). Macrophages over-expressing LXRα exhibited enhanced expression level of MΦ2-type genes and decreased expression level of MΦ1-type genes. Conclusions: PBMCs from healthy persons were predisposed to the MΦ2 differentiation phenotype, which exhibits elevated cholesterol uptake and anti-inflammatory properties. LXRα over-expression polarizes macrophages towards the anti-inflammatory MΦ2 phenotype. |
| first_indexed | 2024-03-11T23:45:55Z |
| format | Article |
| id | doaj.art-57f502a9bca24528bbbd1ec409832f46 |
| institution | Directory Open Access Journal |
| issn | 1064-1963 1525-6006 |
| language | English |
| last_indexed | 2024-03-11T23:45:55Z |
| publishDate | 2018-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Clinical and Experimental Hypertension |
| spelling | doaj.art-57f502a9bca24528bbbd1ec409832f462023-09-19T09:24:45ZengTaylor & Francis GroupClinical and Experimental Hypertension1064-19631525-60062018-04-0140321321710.1080/10641963.2017.12887401288740LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotypeMengyuan Liu0Weijian Yang1Shuling Liu2Duncan Hock3Bohao Zhang4Ruth Ya Huo5Xiaoguang Tong6Hua Yan7Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative DiseasesGraduate School of Tianjin Medical UniversityTianjin Huanhu HospitalUniversity of South FloridaTianjin Huanhu HospitalUniversity of New South WalesTianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative DiseasesTianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative DiseasesObjectives: (1) To investigate the expression patterns of MΦ1 and MΦ2 phenotype markers of peripheral blood monocyte (PBMC)-derived macrophages in atherosclerosis patients and healthy controls, as well as the expression correlation among these genes. (2) To elucidate whether a high level of liver X receptor α (LXRα) expression is associated with anti-inflammatory MΦ2-type polarization. Design: Peripheral blood monocytes (PBMCs) were obtained from 28 patients with carotid artery plaques and 10 normal persons, who did not have carotid artery plaques. M1 and M2 phenotype markers were analyzed after cellular differentiation into macrophages. Human macrophages derived from healthy donors were transfected with plasmid DNA encoding LXRα and control null-plasmids. Gene expression levels were quantified after further differentiation. Results: Three genes (LXRα, CD68, and CD36) were expressed at a significantly lower rate in the atherosclerotic group than normal patients. There were correlations between the expression of LXRα, CD68, and peroxisome proliferator-activated receptor (PPARγ), and between CD163, CD36 and scavenger receptor class A (SRA1). Macrophages over-expressing LXRα exhibited enhanced expression level of MΦ2-type genes and decreased expression level of MΦ1-type genes. Conclusions: PBMCs from healthy persons were predisposed to the MΦ2 differentiation phenotype, which exhibits elevated cholesterol uptake and anti-inflammatory properties. LXRα over-expression polarizes macrophages towards the anti-inflammatory MΦ2 phenotype.http://dx.doi.org/10.1080/10641963.2017.1288740atherosclerosismacrophagepolarizationinflammationliver x receptor (lxrα)peroxisome proliferator-activated receptor (pparγ) |
| spellingShingle | Mengyuan Liu Weijian Yang Shuling Liu Duncan Hock Bohao Zhang Ruth Ya Huo Xiaoguang Tong Hua Yan LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype Clinical and Experimental Hypertension atherosclerosis macrophage polarization inflammation liver x receptor (lxrα) peroxisome proliferator-activated receptor (pparγ) |
| title | LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype |
| title_full | LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype |
| title_fullStr | LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype |
| title_full_unstemmed | LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype |
| title_short | LXRα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over-expression polarizes macrophages towards an anti-inflammatory MΦ2 phenotype |
| title_sort | lxrα is expressed at higher levels in healthy people compared to atherosclerosis patients and its over expression polarizes macrophages towards an anti inflammatory mφ2 phenotype |
| topic | atherosclerosis macrophage polarization inflammation liver x receptor (lxrα) peroxisome proliferator-activated receptor (pparγ) |
| url | http://dx.doi.org/10.1080/10641963.2017.1288740 |
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