35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed
AbstractA mutation in the ovine ABCG2 gene could influence the fat content and milk yield in sheep. The aim of this study was to identify a 35-bp deletion/insertion in intron 5 of the ABCG2 gene in two herds of the Bulgarian Dairy Synthetic Population (BDSP) breed. The first population was raised in...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2022.2107949 |
| _version_ | 1797974125272104960 |
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| author | Milena Bozhilova-Sakova Ivona Dimitrova Nevyana Stancheva Maya Ignatova Tanya Ivanova |
| author_facet | Milena Bozhilova-Sakova Ivona Dimitrova Nevyana Stancheva Maya Ignatova Tanya Ivanova |
| author_sort | Milena Bozhilova-Sakova |
| collection | DOAJ |
| description | AbstractA mutation in the ovine ABCG2 gene could influence the fat content and milk yield in sheep. The aim of this study was to identify a 35-bp deletion/insertion in intron 5 of the ABCG2 gene in two herds of the Bulgarian Dairy Synthetic Population (BDSP) breed. The first population was raised in the Agricultural Institute in Shumen and included 116 ewes. The second one was bred at the Institute of Animal Science – Kostinbrod and included 68 ewes. Genomic DNA was extracted from a total of 184 blood samples. The allelic variants were determined by polymerase chain reaction (PCR) amplification with a specific primer set. The results revealed the presence of the wild-type allele “+” with a frequency of 0.53 and the mutant allele “-” with a frequency of 0.47 in BDSP - Shumen. In the animals from BDSP - Kostinbrod, the wild-type allele “+” had a frequency of 0.71 and the mutant allele “-”, a frequency of 0.29. All three possible genotypes were identified in both herds. In BDSP ewes - Shumen, the wild-type genotype “+/+” had a frequency of 0.22; the heterozygous genotype, a frequency of 0.63; and the mutant genotype “-/-”, a frequency of 0.15. In BDSP - Kostinbrod, the wild-type genotype “+/+” had a frequency of 0.55, the heterozygous genotype “+/-”, 0.32; and the mutant genotype “-/-”, 0.13. Both tested herds were not consistent with the Hardy-Weinberg equilibrium (HWE). In BDSP – Shumen p = 0.01 and in BDSP – Kostinbrod p = 0.05. |
| first_indexed | 2024-04-11T04:15:07Z |
| format | Article |
| id | doaj.art-57f55dc0447d45febe04f4988cf6b63a |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-11T04:15:07Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-57f55dc0447d45febe04f4988cf6b63a2022-12-31T18:03:17ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302022-12-0136171772310.1080/13102818.2022.210794935-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breedMilena Bozhilova-Sakova0Ivona Dimitrova1Nevyana Stancheva2Maya Ignatova3Tanya Ivanova4Department of Genetics, Breeding and Reproduction, Institute of Animal Science, Agricultural Academy, Kostinbrod, BulgariaAgronomy Faculty, Department of Plant Protection, University of Forestry, Sofia, BulgariaDepartment of Animal Science, Agricultural Academy, Agricultural Institute, Shumen, BulgariaDepartment of Genetics, Breeding and Reproduction, Institute of Animal Science, Agricultural Academy, Kostinbrod, BulgariaDepartment of Genetics, Breeding and Reproduction, Institute of Animal Science, Agricultural Academy, Kostinbrod, BulgariaAbstractA mutation in the ovine ABCG2 gene could influence the fat content and milk yield in sheep. The aim of this study was to identify a 35-bp deletion/insertion in intron 5 of the ABCG2 gene in two herds of the Bulgarian Dairy Synthetic Population (BDSP) breed. The first population was raised in the Agricultural Institute in Shumen and included 116 ewes. The second one was bred at the Institute of Animal Science – Kostinbrod and included 68 ewes. Genomic DNA was extracted from a total of 184 blood samples. The allelic variants were determined by polymerase chain reaction (PCR) amplification with a specific primer set. The results revealed the presence of the wild-type allele “+” with a frequency of 0.53 and the mutant allele “-” with a frequency of 0.47 in BDSP - Shumen. In the animals from BDSP - Kostinbrod, the wild-type allele “+” had a frequency of 0.71 and the mutant allele “-”, a frequency of 0.29. All three possible genotypes were identified in both herds. In BDSP ewes - Shumen, the wild-type genotype “+/+” had a frequency of 0.22; the heterozygous genotype, a frequency of 0.63; and the mutant genotype “-/-”, a frequency of 0.15. In BDSP - Kostinbrod, the wild-type genotype “+/+” had a frequency of 0.55, the heterozygous genotype “+/-”, 0.32; and the mutant genotype “-/-”, 0.13. Both tested herds were not consistent with the Hardy-Weinberg equilibrium (HWE). In BDSP – Shumen p = 0.01 and in BDSP – Kostinbrod p = 0.05.https://www.tandfonline.com/doi/10.1080/13102818.2022.2107949ABCG2 genesheepBulgarian Dairy Synthetic Population breed35 bp deletion/insertion |
| spellingShingle | Milena Bozhilova-Sakova Ivona Dimitrova Nevyana Stancheva Maya Ignatova Tanya Ivanova 35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed Biotechnology & Biotechnological Equipment ABCG2 gene sheep Bulgarian Dairy Synthetic Population breed 35 bp deletion/insertion |
| title | 35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed |
| title_full | 35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed |
| title_fullStr | 35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed |
| title_full_unstemmed | 35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed |
| title_short | 35-bp deletion in ABCG2 gene: mini-review and report on two herds of Bulgarian dairy synthetic population sheep breed |
| title_sort | 35 bp deletion in abcg2 gene mini review and report on two herds of bulgarian dairy synthetic population sheep breed |
| topic | ABCG2 gene sheep Bulgarian Dairy Synthetic Population breed 35 bp deletion/insertion |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2022.2107949 |
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