Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease
Abstract Background Chronic obstructive pulmonary disease (COPD) affecting 334 million people in the world remains a major cause of morbidity and mortality. Proper diagnosis of COPD is still a challenge and largely solely based on spirometric criteria. We aimed to investigate the potential of nitros...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Journal of Occupational Medicine and Toxicology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12995-024-00409-6 |
| _version_ | 1797219457821573120 |
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| author | Romain Freund Jean‑Jacques Sauvain Guillaume Suarez Pascal Wild Thomas Charreau Amélie Debatisse Kirushanthi Sakthithasan Valérie Jouannique Jacques A. Pralong Irina Guseva Canu |
| author_facet | Romain Freund Jean‑Jacques Sauvain Guillaume Suarez Pascal Wild Thomas Charreau Amélie Debatisse Kirushanthi Sakthithasan Valérie Jouannique Jacques A. Pralong Irina Guseva Canu |
| author_sort | Romain Freund |
| collection | DOAJ |
| description | Abstract Background Chronic obstructive pulmonary disease (COPD) affecting 334 million people in the world remains a major cause of morbidity and mortality. Proper diagnosis of COPD is still a challenge and largely solely based on spirometric criteria. We aimed to investigate the potential of nitrosative/oxidative stress and related metabolic biomarkers in exhaled breath condensate (EBC) to discriminate COPD patients. Methods Three hundred three participants were randomly selected from a 15,000-transit worker cohort within the Respiratory disease Occupational Biomonitoring Collaborative Project (ROBoCoP). COPD was defined using the Global Initiative for Chronic Obstructive Lung Disease (GOLD) criteria as post-bronchodilator ratio of Forced Expiratory Volume in 1st second to Forced Vital Capacity < 0.7 in spirometry validated by an experienced pulmonologist. Discriminative power of biomarker profiles in EBC was analyzed using linear discriminant analyses. Results Amongst 300 participants with validated spirometry, 50.3% were female, 52.3 years old in average, 36.0% were current smokers, 12.7% ex-smokers with mean tobacco exposure of 15.4 pack-years. Twenty-one participants (7.0%) were diagnosed as COPD, including 19 new diagnoses, 12 of which with a mild COPD stage (GOLD 1). Amongst 8 biomarkers measured in EBC, combination of 2 biomarkers, Lactate and Malondialdehyde (MDA) significantly discriminated COPD subjects from non-COPD, with a 71%-accuracy, area under the receiver curve of 0.78 (p-value < 0.001), and a negative predictive value of 96%. Conclusions These findings support the potential of biomarkers in EBC, in particular lactate and MDA, to discriminate COPD patients even at a mild or moderate stage. These EBC biomarkers present a non-invasive and drugless technique, which can improve COPD diagnosis in the future. |
| first_indexed | 2024-04-24T12:33:57Z |
| format | Article |
| id | doaj.art-57f5ff83ce2b4716b57991cf9b0981f6 |
| institution | Directory Open Access Journal |
| issn | 1745-6673 |
| language | English |
| last_indexed | 2024-04-24T12:33:57Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Occupational Medicine and Toxicology |
| spelling | doaj.art-57f5ff83ce2b4716b57991cf9b0981f62024-04-07T11:34:47ZengBMCJournal of Occupational Medicine and Toxicology1745-66732024-04-0119111010.1186/s12995-024-00409-6Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary diseaseRomain Freund0Jean‑Jacques Sauvain1Guillaume Suarez2Pascal Wild3Thomas Charreau4Amélie Debatisse5Kirushanthi Sakthithasan6Valérie Jouannique7Jacques A. Pralong8Irina Guseva Canu9Department of Occupational and Environmental Health (DSTE), Centre for Primary Care and Public Health (Unisanté), University of LausanneDepartment of Occupational and Environmental Health (DSTE), Centre for Primary Care and Public Health (Unisanté), University of LausanneDepartment of Occupational and Environmental Health (DSTE), Centre for Primary Care and Public Health (Unisanté), University of LausanneDepartment of Occupational and Environmental Health (DSTE), Centre for Primary Care and Public Health (Unisanté), University of LausanneDepartment of Occupational and Environmental Health (DSTE), Centre for Primary Care and Public Health (Unisanté), University of LausanneAutonomous Paris Transport Authority (RATP)Autonomous Paris Transport Authority (RATP)Autonomous Paris Transport Authority (RATP)Faculty of Medicine, University of GenevaDepartment of Occupational and Environmental Health (DSTE), Centre for Primary Care and Public Health (Unisanté), University of LausanneAbstract Background Chronic obstructive pulmonary disease (COPD) affecting 334 million people in the world remains a major cause of morbidity and mortality. Proper diagnosis of COPD is still a challenge and largely solely based on spirometric criteria. We aimed to investigate the potential of nitrosative/oxidative stress and related metabolic biomarkers in exhaled breath condensate (EBC) to discriminate COPD patients. Methods Three hundred three participants were randomly selected from a 15,000-transit worker cohort within the Respiratory disease Occupational Biomonitoring Collaborative Project (ROBoCoP). COPD was defined using the Global Initiative for Chronic Obstructive Lung Disease (GOLD) criteria as post-bronchodilator ratio of Forced Expiratory Volume in 1st second to Forced Vital Capacity < 0.7 in spirometry validated by an experienced pulmonologist. Discriminative power of biomarker profiles in EBC was analyzed using linear discriminant analyses. Results Amongst 300 participants with validated spirometry, 50.3% were female, 52.3 years old in average, 36.0% were current smokers, 12.7% ex-smokers with mean tobacco exposure of 15.4 pack-years. Twenty-one participants (7.0%) were diagnosed as COPD, including 19 new diagnoses, 12 of which with a mild COPD stage (GOLD 1). Amongst 8 biomarkers measured in EBC, combination of 2 biomarkers, Lactate and Malondialdehyde (MDA) significantly discriminated COPD subjects from non-COPD, with a 71%-accuracy, area under the receiver curve of 0.78 (p-value < 0.001), and a negative predictive value of 96%. Conclusions These findings support the potential of biomarkers in EBC, in particular lactate and MDA, to discriminate COPD patients even at a mild or moderate stage. These EBC biomarkers present a non-invasive and drugless technique, which can improve COPD diagnosis in the future.https://doi.org/10.1186/s12995-024-00409-6COPDRespiratoryExhaled breath condensateBiomarkerOxidative stress |
| spellingShingle | Romain Freund Jean‑Jacques Sauvain Guillaume Suarez Pascal Wild Thomas Charreau Amélie Debatisse Kirushanthi Sakthithasan Valérie Jouannique Jacques A. Pralong Irina Guseva Canu Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease Journal of Occupational Medicine and Toxicology COPD Respiratory Exhaled breath condensate Biomarker Oxidative stress |
| title | Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease |
| title_full | Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease |
| title_fullStr | Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease |
| title_full_unstemmed | Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease |
| title_short | Discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease |
| title_sort | discriminative potential of exhaled breath condensate biomarkers with respect to chronic obstructive pulmonary disease |
| topic | COPD Respiratory Exhaled breath condensate Biomarker Oxidative stress |
| url | https://doi.org/10.1186/s12995-024-00409-6 |
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