External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling
A discrepancy between cytology and bacterial culture methods is sometimes observed in canine otitis externa. The objective of this study was to compare results from cytology, bacterial culture and 16S amplicon profiling. Twenty samples from 16 dogs with chronic suppurative otitis externa were collec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/7/366 |
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| author | Caroline Leonard Damien Thiry Bernard Taminiau Georges Daube Jacques Fontaine |
| author_facet | Caroline Leonard Damien Thiry Bernard Taminiau Georges Daube Jacques Fontaine |
| author_sort | Caroline Leonard |
| collection | DOAJ |
| description | A discrepancy between cytology and bacterial culture methods is sometimes observed in canine otitis externa. The objective of this study was to compare results from cytology, bacterial culture and 16S amplicon profiling. Twenty samples from 16 dogs with chronic suppurative otitis externa were collected. A direct cytological evaluation was carried out during the consultations. Aerobic bacterial culture and susceptibility were performed by an external laboratory used in routine practice. For 16S amplicon profiling, DNA was extracted and the hypervariable segment V1–V3 of the 16S rDNA was amplified and then sequenced with a MiSeq Illumina sequence carried out by the Mothur software using the SILVA database. A good correlation between cytology and bacterial culture was observed in 60% of the samples. Some bacterial species revealed by bacterial culture were present with low relative abundance (<10%) in 16S amplicon profiling. Some bacterial species revealed by the 16S amplicon profiling analysis were not identified with culture; most of the time, the offending species was a <i>Corynebacterium</i>. To conclude, a careful interpretation of the results of bacterial culture should be made and always be in agreement with the cytology. The 16S amplicon profiling method appears to be a more sensitive method for detecting strains present in suppurative otitis but does not provide information on bacterial susceptibility. |
| first_indexed | 2024-03-09T12:51:53Z |
| format | Article |
| id | doaj.art-57f6f286102748c6af21a9ff8ac913a6 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T12:51:53Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-57f6f286102748c6af21a9ff8ac913a62023-11-30T22:04:54ZengMDPI AGVeterinary Sciences2306-73812022-07-019736610.3390/vetsci9070366External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon ProfilingCaroline Leonard0Damien Thiry1Bernard Taminiau2Georges Daube3Jacques Fontaine4Department for Clinical Sciences, Faculté de Médecine Vétérinaire, University of Liege, 4000 Liege, BelgiumBacteriology Laboratory, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumFundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine B43b, University of Liege, 4000 Liege, BelgiumFundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine B43b, University of Liege, 4000 Liege, BelgiumDepartment for Clinical Sciences, Faculté de Médecine Vétérinaire, University of Liege, 4000 Liege, BelgiumA discrepancy between cytology and bacterial culture methods is sometimes observed in canine otitis externa. The objective of this study was to compare results from cytology, bacterial culture and 16S amplicon profiling. Twenty samples from 16 dogs with chronic suppurative otitis externa were collected. A direct cytological evaluation was carried out during the consultations. Aerobic bacterial culture and susceptibility were performed by an external laboratory used in routine practice. For 16S amplicon profiling, DNA was extracted and the hypervariable segment V1–V3 of the 16S rDNA was amplified and then sequenced with a MiSeq Illumina sequence carried out by the Mothur software using the SILVA database. A good correlation between cytology and bacterial culture was observed in 60% of the samples. Some bacterial species revealed by bacterial culture were present with low relative abundance (<10%) in 16S amplicon profiling. Some bacterial species revealed by the 16S amplicon profiling analysis were not identified with culture; most of the time, the offending species was a <i>Corynebacterium</i>. To conclude, a careful interpretation of the results of bacterial culture should be made and always be in agreement with the cytology. The 16S amplicon profiling method appears to be a more sensitive method for detecting strains present in suppurative otitis but does not provide information on bacterial susceptibility.https://www.mdpi.com/2306-7381/9/7/366suppurative otitis externa16S amplicon profilingbacterial culturebacterial susceptibilitycanine |
| spellingShingle | Caroline Leonard Damien Thiry Bernard Taminiau Georges Daube Jacques Fontaine External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling Veterinary Sciences suppurative otitis externa 16S amplicon profiling bacterial culture bacterial susceptibility canine |
| title | External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling |
| title_full | External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling |
| title_fullStr | External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling |
| title_full_unstemmed | External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling |
| title_short | External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling |
| title_sort | external ear canal evaluation in dogs with chronic suppurative otitis externa comparison of direct cytology bacterial culture and 16s amplicon profiling |
| topic | suppurative otitis externa 16S amplicon profiling bacterial culture bacterial susceptibility canine |
| url | https://www.mdpi.com/2306-7381/9/7/366 |
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