Dendritic mitochondria reach stable positions during circuit development
Mitochondria move throughout neuronal dendrites and localize to sites of energy demand. The prevailing view of dendritic mitochondria as highly motile organelles whose distribution is continually adjusted by neuronal activity via Ca2+-dependent arrests is based on observations in cultured neurons ex...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/11583 |
| _version_ | 1811236874802429952 |
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| author | Michelle C Faits Chunmeng Zhang Florentina Soto Daniel Kerschensteiner |
| author_facet | Michelle C Faits Chunmeng Zhang Florentina Soto Daniel Kerschensteiner |
| author_sort | Michelle C Faits |
| collection | DOAJ |
| description | Mitochondria move throughout neuronal dendrites and localize to sites of energy demand. The prevailing view of dendritic mitochondria as highly motile organelles whose distribution is continually adjusted by neuronal activity via Ca2+-dependent arrests is based on observations in cultured neurons exposed to artificial stimuli. Here, we analyze the movements of mitochondria in ganglion cell dendrites in the intact retina. We find that whereas during development 30% of mitochondria are motile at any time, as dendrites mature, mitochondria all but stop moving and localize stably to synapses and branch points. Neither spontaneous nor sensory-evoked activity and Ca2+ transients alter motility of dendritic mitochondria; and pathological hyperactivity in a mouse model of retinal degeneration elevates rather than reduces motility. Thus, our findings indicate that dendritic mitochondria reach stable positions during a critical developmental period of high motility, and challenge current views about the role of activity in regulating mitochondrial transport in dendrites. |
| first_indexed | 2024-04-12T12:15:38Z |
| format | Article |
| id | doaj.art-57fa62910aa24dfb8ea1b7ba15bd93f3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:15:38Z |
| publishDate | 2016-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-57fa62910aa24dfb8ea1b7ba15bd93f32022-12-22T03:33:26ZengeLife Sciences Publications LtdeLife2050-084X2016-01-01510.7554/eLife.11583Dendritic mitochondria reach stable positions during circuit developmentMichelle C Faits0Chunmeng Zhang1Florentina Soto2Daniel Kerschensteiner3Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, Saint Louis, United States; Graduate Program in Developmental, Regenerative and Stem Cell Biology, Washington University School of Medicine, St. Louis, United StatesDepartment of Ophthalmology and Visual Sciences, Washington University School of Medicine, Saint Louis, United StatesDepartment of Ophthalmology and Visual Sciences, Washington University School of Medicine, Saint Louis, United StatesDepartment of Ophthalmology and Visual Sciences, Washington University School of Medicine, Saint Louis, United States; Department of Neuroscience, Washington University School of Medicine, Saint Louis, United States; Department of Biomedical Engineering, Washington University School of Medicine, Saint Louis, United States; Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, United StatesMitochondria move throughout neuronal dendrites and localize to sites of energy demand. The prevailing view of dendritic mitochondria as highly motile organelles whose distribution is continually adjusted by neuronal activity via Ca2+-dependent arrests is based on observations in cultured neurons exposed to artificial stimuli. Here, we analyze the movements of mitochondria in ganglion cell dendrites in the intact retina. We find that whereas during development 30% of mitochondria are motile at any time, as dendrites mature, mitochondria all but stop moving and localize stably to synapses and branch points. Neither spontaneous nor sensory-evoked activity and Ca2+ transients alter motility of dendritic mitochondria; and pathological hyperactivity in a mouse model of retinal degeneration elevates rather than reduces motility. Thus, our findings indicate that dendritic mitochondria reach stable positions during a critical developmental period of high motility, and challenge current views about the role of activity in regulating mitochondrial transport in dendrites.https://elifesciences.org/articles/11583mitochondriadendritesretinal circuitryneuron |
| spellingShingle | Michelle C Faits Chunmeng Zhang Florentina Soto Daniel Kerschensteiner Dendritic mitochondria reach stable positions during circuit development eLife mitochondria dendrites retinal circuitry neuron |
| title | Dendritic mitochondria reach stable positions during circuit development |
| title_full | Dendritic mitochondria reach stable positions during circuit development |
| title_fullStr | Dendritic mitochondria reach stable positions during circuit development |
| title_full_unstemmed | Dendritic mitochondria reach stable positions during circuit development |
| title_short | Dendritic mitochondria reach stable positions during circuit development |
| title_sort | dendritic mitochondria reach stable positions during circuit development |
| topic | mitochondria dendrites retinal circuitry neuron |
| url | https://elifesciences.org/articles/11583 |
| work_keys_str_mv | AT michellecfaits dendriticmitochondriareachstablepositionsduringcircuitdevelopment AT chunmengzhang dendriticmitochondriareachstablepositionsduringcircuitdevelopment AT florentinasoto dendriticmitochondriareachstablepositionsduringcircuitdevelopment AT danielkerschensteiner dendriticmitochondriareachstablepositionsduringcircuitdevelopment |