| Summary: | Sustainable management of agricultural resources is needed to meet people’s increasing demands for food, fiber and energy while maintaining the quality of the environment and protecting natural resources. With the rapid growth of agriculture and the mechanization of farming, the agricultural sector has become one of the main contributors to the increase in CO<sub>2</sub> emissions and other greenhouse gases in the world. Therefore, the aim of this study was to determine the effect, dependence and correlations of CO<sub>2</sub> in soil with native vegetation (presence/absence, corn yield) and climatic conditions (soil temperature and moisture) during three years of measurements under different management practices in a classical conventional agroecosystem. This research contains four different treatments: control treatment (CT), dolomite/organic fertilization (DOL/OF), mineral fertilization (MF) and black fallow (BF). During the investigated period, the average overall C-CO<sub>2</sub> flux ranged from 7.98 kg ha<sup>−1</sup> day<sup>−1</sup> on bare soil to 16.26 kg ha<sup>−1</sup> day<sup>−1</sup> on soil treated with mineral fertilization. No statistically significant difference was observed among different fertilization treatments, except in 2013 and 2015 when comparing different fertilization treatments to bare soil. In all three years, there was a positive correlation between average C-CO<sub>2</sub> fluxes and soil temperature. Additionally, in 2013 and 2017, there was a positive correlation between average C-CO<sub>2</sub> fluxes and soil moisture, while a negative correlation was observed in 2015. Obtained values of crop yield ranged from 0.89 t ha<sup>−1</sup> in the control treatment (in 2015) to 14.81 t ha<sup>−1</sup> in the treatment with mineral fertilization (in 2017). Growing global concern about the effects of climate change calls for intensive research on the carbon cycle, and these results will contribute to the understanding of carbon transformation in different crops and soil management practices.
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