Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis
In recent years, high-throughput lipid profiling has contributed to understand the biological, physiological and pathological roles of lipids in living organisms. Across all kingdoms of life, important cell and systemic processes are mediated by lipids including compartmentalization, signaling and e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/6/231 |
| _version_ | 1797566158552956928 |
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| author | Julian Aldana Adriana Romero-Otero Mónica P. Cala |
| author_facet | Julian Aldana Adriana Romero-Otero Mónica P. Cala |
| author_sort | Julian Aldana |
| collection | DOAJ |
| description | In recent years, high-throughput lipid profiling has contributed to understand the biological, physiological and pathological roles of lipids in living organisms. Across all kingdoms of life, important cell and systemic processes are mediated by lipids including compartmentalization, signaling and energy homeostasis. Despite important advances in liquid chromatography and mass spectrometry, sample extraction procedures remain a bottleneck in lipidomic studies, since the wide structural diversity of lipids imposes a constrain in the type and amount of lipids extracted. Differences in extraction yield across lipid classes can induce a bias on down-stream analysis and outcomes. This review aims to summarize current lipid extraction techniques used for untargeted and targeted studies based on mass spectrometry. Considerations, applications, and limitations of these techniques are discussed when used to extract lipids in complex biological matrices, such as tissues, biofluids, foods, and microorganisms. |
| first_indexed | 2024-03-10T19:23:42Z |
| format | Article |
| id | doaj.art-57fe34ccf65843d799d78b2d4bb9518f |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T19:23:42Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-57fe34ccf65843d799d78b2d4bb9518f2023-11-20T02:46:40ZengMDPI AGMetabolites2218-19892020-06-0110623110.3390/metabo10060231Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry AnalysisJulian Aldana0Adriana Romero-Otero1Mónica P. Cala2Metabolomics Core Facility–MetCore, Vicepresidency for Research, Universidad de los Andes, Cra 1 No. 18A—12, Bogota 111711, ColombiaMetabolomics Core Facility–MetCore, Vicepresidency for Research, Universidad de los Andes, Cra 1 No. 18A—12, Bogota 111711, ColombiaMetabolomics Core Facility–MetCore, Vicepresidency for Research, Universidad de los Andes, Cra 1 No. 18A—12, Bogota 111711, ColombiaIn recent years, high-throughput lipid profiling has contributed to understand the biological, physiological and pathological roles of lipids in living organisms. Across all kingdoms of life, important cell and systemic processes are mediated by lipids including compartmentalization, signaling and energy homeostasis. Despite important advances in liquid chromatography and mass spectrometry, sample extraction procedures remain a bottleneck in lipidomic studies, since the wide structural diversity of lipids imposes a constrain in the type and amount of lipids extracted. Differences in extraction yield across lipid classes can induce a bias on down-stream analysis and outcomes. This review aims to summarize current lipid extraction techniques used for untargeted and targeted studies based on mass spectrometry. Considerations, applications, and limitations of these techniques are discussed when used to extract lipids in complex biological matrices, such as tissues, biofluids, foods, and microorganisms.https://www.mdpi.com/2218-1989/10/6/231extractionlipidsuntargeted lipidomicstargeted lipidomics |
| spellingShingle | Julian Aldana Adriana Romero-Otero Mónica P. Cala Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis Metabolites extraction lipids untargeted lipidomics targeted lipidomics |
| title | Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis |
| title_full | Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis |
| title_fullStr | Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis |
| title_full_unstemmed | Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis |
| title_short | Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis |
| title_sort | exploring the lipidome current lipid extraction techniques for mass spectrometry analysis |
| topic | extraction lipids untargeted lipidomics targeted lipidomics |
| url | https://www.mdpi.com/2218-1989/10/6/231 |
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