Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues
Gold nanoparticles (AuNPs) have a wide-ranging application and are widespread in samples with complex matrices; thus, efficient analytical procedures are necessary to identify and characterize this analyte. A sensitive analytical method for determination of AuNPs content in biological tissues, based...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/11/12/3370 |
| _version_ | 1797501979167031296 |
|---|---|
| author | Oana Cadar Teodora Mocan Cecilia Roman Marin Senila |
| author_facet | Oana Cadar Teodora Mocan Cecilia Roman Marin Senila |
| author_sort | Oana Cadar |
| collection | DOAJ |
| description | Gold nanoparticles (AuNPs) have a wide-ranging application and are widespread in samples with complex matrices; thus, efficient analytical procedures are necessary to identify and characterize this analyte. A sensitive analytical method for determination of AuNPs content in biological tissues, based on microwave-assisted acid wet digestion and graphite furnace atomic absorption spectrometry (GFAAS) validated in accordance with the requirements of Eurachem guideline and ISO 17025 standard, is presented in this study. The digestion procedure was optimized, and the figures of merit such as selectivity, limit of detection (0.43 µg L<sup>−1</sup>), limit of quantification (1.29 µg L<sup>−1</sup>, corresponding to 12.9 µg kg<sup>−1</sup> in tissue sample, considering the digestion), working range, linearity, repeatability ((RSD<sub>r</sub> 4.15%), intermediate precision (RSD<sub>R</sub> 8.07%), recovery in accuracy study (97%), were methodically evaluated. The measurement uncertainty was assessed considering the main sources of uncertainties and the calculated relative expanded uncertainty (k = 2) was 12.5%. The method was applied for the determination of AuNPs in six biological tissues (liver, small intestine, heart, lungs, brain and kidneys) and the found concentrations were generally at low levels, close or lower than LOQ. |
| first_indexed | 2024-03-10T03:26:17Z |
| format | Article |
| id | doaj.art-57ffba24111e41cb8f3bf6975caf078b |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:26:17Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-57ffba24111e41cb8f3bf6975caf078b2023-11-23T09:51:39ZengMDPI AGNanomaterials2079-49912021-12-011112337010.3390/nano11123370Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological TissuesOana Cadar0Teodora Mocan1Cecilia Roman2Marin Senila3INCDO-INOE 2000, Research Institute for Analytical Instrumentation, RO-400296 Cluj-Napoca, RomaniaPhysiology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, RO-400006 Cluj-Napoca, RomaniaINCDO-INOE 2000, Research Institute for Analytical Instrumentation, RO-400296 Cluj-Napoca, RomaniaINCDO-INOE 2000, Research Institute for Analytical Instrumentation, RO-400296 Cluj-Napoca, RomaniaGold nanoparticles (AuNPs) have a wide-ranging application and are widespread in samples with complex matrices; thus, efficient analytical procedures are necessary to identify and characterize this analyte. A sensitive analytical method for determination of AuNPs content in biological tissues, based on microwave-assisted acid wet digestion and graphite furnace atomic absorption spectrometry (GFAAS) validated in accordance with the requirements of Eurachem guideline and ISO 17025 standard, is presented in this study. The digestion procedure was optimized, and the figures of merit such as selectivity, limit of detection (0.43 µg L<sup>−1</sup>), limit of quantification (1.29 µg L<sup>−1</sup>, corresponding to 12.9 µg kg<sup>−1</sup> in tissue sample, considering the digestion), working range, linearity, repeatability ((RSD<sub>r</sub> 4.15%), intermediate precision (RSD<sub>R</sub> 8.07%), recovery in accuracy study (97%), were methodically evaluated. The measurement uncertainty was assessed considering the main sources of uncertainties and the calculated relative expanded uncertainty (k = 2) was 12.5%. The method was applied for the determination of AuNPs in six biological tissues (liver, small intestine, heart, lungs, brain and kidneys) and the found concentrations were generally at low levels, close or lower than LOQ.https://www.mdpi.com/2079-4991/11/12/3370gold nanoparticlesGF-AASvalidationbiological tissue |
| spellingShingle | Oana Cadar Teodora Mocan Cecilia Roman Marin Senila Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues Nanomaterials gold nanoparticles GF-AAS validation biological tissue |
| title | Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues |
| title_full | Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues |
| title_fullStr | Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues |
| title_full_unstemmed | Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues |
| title_short | Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues |
| title_sort | analytical performance and validation of a reliable method based on graphite furnace atomic absorption spectrometry for the determination of gold nanoparticles in biological tissues |
| topic | gold nanoparticles GF-AAS validation biological tissue |
| url | https://www.mdpi.com/2079-4991/11/12/3370 |
| work_keys_str_mv | AT oanacadar analyticalperformanceandvalidationofareliablemethodbasedongraphitefurnaceatomicabsorptionspectrometryforthedeterminationofgoldnanoparticlesinbiologicaltissues AT teodoramocan analyticalperformanceandvalidationofareliablemethodbasedongraphitefurnaceatomicabsorptionspectrometryforthedeterminationofgoldnanoparticlesinbiologicaltissues AT ceciliaroman analyticalperformanceandvalidationofareliablemethodbasedongraphitefurnaceatomicabsorptionspectrometryforthedeterminationofgoldnanoparticlesinbiologicaltissues AT marinsenila analyticalperformanceandvalidationofareliablemethodbasedongraphitefurnaceatomicabsorptionspectrometryforthedeterminationofgoldnanoparticlesinbiologicaltissues |