Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis
The PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not wel...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2016-01-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/53/1/53_0150091/_pdf/-char/en |
| _version_ | 1797816936363458560 |
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| author | Gen Hiyama Norio Kansaku Shin Wakui Rosanne McQuaid David Zadworny |
| author_facet | Gen Hiyama Norio Kansaku Shin Wakui Rosanne McQuaid David Zadworny |
| author_sort | Gen Hiyama |
| collection | DOAJ |
| description | The PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not well understood to date. In this study, we aimed to clone and characterize the turkey PREB gene and investigate its mRNA expression in the anterior pituitary gland and pancreas during embryogenesis. Based on the conserved sequence of chicken and mammalian PREB cDNAs, a turkey PREB cDNA fragment was obtained, and after sequencing of the fragment, the 5′- and 3′-ends of mRNA were amplified and determined. To identify the PREB gene structure, polymerase chain reaction (PCR) amplification was performed. The turkey PREB gene consists of 9 exons and 8 introns, and it encodes a 411-amino-acid protein. The expression of PREB mRNA in the anterior pituitary gland was measured during embryogenesis. Levels of PREB mRNA significantly increased at embryonic day 22, with maximum levels being detected on day 25 of ontogeny, which correlated with similar changes in levels of PRL mRNA. The highest level of PREB mRNA was detected on day 19 in the pancreas. However, the highest level of insulin mRNA was detected at embryonic day 25. These results indicate that PREB may be involved in the expression of PRL mRNA in the anterior pituitary gland, whereas insulin mRNA may be expressed independently of the expression of PREB mRNA in the pancreas during embryogenesis. |
| first_indexed | 2024-03-13T08:46:03Z |
| format | Article |
| id | doaj.art-58008fd9d66042d4b9bf028112b54a04 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:03Z |
| publishDate | 2016-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-58008fd9d66042d4b9bf028112b54a042023-05-30T04:25:49ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862016-01-01531677510.2141/jpsa.0150091jpsaCharacterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During EmbryogenesisGen Hiyama0Norio Kansaku1Shin Wakui2Rosanne McQuaid3David Zadworny4Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, JapanDepartment of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, JapanDepartment of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, JapanDepartment of Animal Science, McGill University, CanadaDepartment of Animal Science, McGill University, CanadaThe PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not well understood to date. In this study, we aimed to clone and characterize the turkey PREB gene and investigate its mRNA expression in the anterior pituitary gland and pancreas during embryogenesis. Based on the conserved sequence of chicken and mammalian PREB cDNAs, a turkey PREB cDNA fragment was obtained, and after sequencing of the fragment, the 5′- and 3′-ends of mRNA were amplified and determined. To identify the PREB gene structure, polymerase chain reaction (PCR) amplification was performed. The turkey PREB gene consists of 9 exons and 8 introns, and it encodes a 411-amino-acid protein. The expression of PREB mRNA in the anterior pituitary gland was measured during embryogenesis. Levels of PREB mRNA significantly increased at embryonic day 22, with maximum levels being detected on day 25 of ontogeny, which correlated with similar changes in levels of PRL mRNA. The highest level of PREB mRNA was detected on day 19 in the pancreas. However, the highest level of insulin mRNA was detected at embryonic day 25. These results indicate that PREB may be involved in the expression of PRL mRNA in the anterior pituitary gland, whereas insulin mRNA may be expressed independently of the expression of PREB mRNA in the pancreas during embryogenesis.https://www.jstage.jst.go.jp/article/jpsa/53/1/53_0150091/_pdf/-char/enmrnapancreaspituitary glandpreb |
| spellingShingle | Gen Hiyama Norio Kansaku Shin Wakui Rosanne McQuaid David Zadworny Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis The Journal of Poultry Science mrna pancreas pituitary gland preb |
| title | Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis |
| title_full | Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis |
| title_fullStr | Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis |
| title_full_unstemmed | Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis |
| title_short | Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis |
| title_sort | characterization and expression of turkey prolactin regulatory element binding in the anterior pituitary gland and pancreas during embryogenesis |
| topic | mrna pancreas pituitary gland preb |
| url | https://www.jstage.jst.go.jp/article/jpsa/53/1/53_0150091/_pdf/-char/en |
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