Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways
Introduction: Recent studies in our laboratory have shown that some bile acids, such as chenodeoxycholic acid (CDCA), can exert cellular protective effects when encapsulated with viable β-cells via anti-inflammatory and anti-oxidative stress mechanisms. However, to explore their full potential, form...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/8/1184 |
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| author | Armin Mooranian Thomas Foster Corina M. Ionescu Daniel Walker Melissa Jones Susbin Raj Wagle Bozica Kovacevic Jacqueline Chester Edan Johnston Elaine Wong Marcus D. Atlas Momir Mikov Hani Al-Salami |
| author_facet | Armin Mooranian Thomas Foster Corina M. Ionescu Daniel Walker Melissa Jones Susbin Raj Wagle Bozica Kovacevic Jacqueline Chester Edan Johnston Elaine Wong Marcus D. Atlas Momir Mikov Hani Al-Salami |
| author_sort | Armin Mooranian |
| collection | DOAJ |
| description | Introduction: Recent studies in our laboratory have shown that some bile acids, such as chenodeoxycholic acid (CDCA), can exert cellular protective effects when encapsulated with viable β-cells via anti-inflammatory and anti-oxidative stress mechanisms. However, to explore their full potential, formulating such bile acids (that are intrinsically lipophilic) can be challenging, particularly if larger doses are required for optimal pharmacological effects. One promising approach is the development of nano gels. Accordingly, this study aimed to examine biological effects of various concentrations of CDCA using various solubilising nano gel systems on encapsulated β-cells. Methods: Using our established cellular encapsulation system, the Ionic Gelation Vibrational Jet Flow technology, a wide range of CDCA β-cell capsules were produced and examined for morphological, biological, and inflammatory profiles. Results and Conclusion: Capsules’ morphology and topographic characteristics remained similar, regardless of CDCA or nano gel concentrations. The best pharmacological, anti-inflammatory, and cellular respiration, metabolism, and energy production effects were observed at high CDCA and nano gel concentrations, suggesting dose-dependent cellular protective and positive effects of CDCA when incorporated with high loading nano gel. |
| first_indexed | 2024-03-10T08:29:12Z |
| format | Article |
| id | doaj.art-5801f58b8fb84bd4abefbb82ca77218e |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T08:29:12Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-5801f58b8fb84bd4abefbb82ca77218e2023-11-22T09:13:56ZengMDPI AGPharmaceutics1999-49232021-07-01138118410.3390/pharmaceutics13081184Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification PathwaysArmin Mooranian0Thomas Foster1Corina M. Ionescu2Daniel Walker3Melissa Jones4Susbin Raj Wagle5Bozica Kovacevic6Jacqueline Chester7Edan Johnston8Elaine Wong9Marcus D. Atlas10Momir Mikov11Hani Al-Salami12The Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaHearing Therapeutics, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Perth, WA 6009, AustraliaHearing Therapeutics, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Perth, WA 6009, AustraliaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21101 Novi Sad, SerbiaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, AustraliaIntroduction: Recent studies in our laboratory have shown that some bile acids, such as chenodeoxycholic acid (CDCA), can exert cellular protective effects when encapsulated with viable β-cells via anti-inflammatory and anti-oxidative stress mechanisms. However, to explore their full potential, formulating such bile acids (that are intrinsically lipophilic) can be challenging, particularly if larger doses are required for optimal pharmacological effects. One promising approach is the development of nano gels. Accordingly, this study aimed to examine biological effects of various concentrations of CDCA using various solubilising nano gel systems on encapsulated β-cells. Methods: Using our established cellular encapsulation system, the Ionic Gelation Vibrational Jet Flow technology, a wide range of CDCA β-cell capsules were produced and examined for morphological, biological, and inflammatory profiles. Results and Conclusion: Capsules’ morphology and topographic characteristics remained similar, regardless of CDCA or nano gel concentrations. The best pharmacological, anti-inflammatory, and cellular respiration, metabolism, and energy production effects were observed at high CDCA and nano gel concentrations, suggesting dose-dependent cellular protective and positive effects of CDCA when incorporated with high loading nano gel.https://www.mdpi.com/1999-4923/13/8/1184bile acidsβ-cellsmicrocapsulesdiabetes mellituschenodeoxycholic acidnano gel |
| spellingShingle | Armin Mooranian Thomas Foster Corina M. Ionescu Daniel Walker Melissa Jones Susbin Raj Wagle Bozica Kovacevic Jacqueline Chester Edan Johnston Elaine Wong Marcus D. Atlas Momir Mikov Hani Al-Salami Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways Pharmaceutics bile acids β-cells microcapsules diabetes mellitus chenodeoxycholic acid nano gel |
| title | Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways |
| title_full | Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways |
| title_fullStr | Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways |
| title_full_unstemmed | Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways |
| title_short | Enhanced Bilosomal Properties Resulted in Optimum Pharmacological Effects by Increased Acidification Pathways |
| title_sort | enhanced bilosomal properties resulted in optimum pharmacological effects by increased acidification pathways |
| topic | bile acids β-cells microcapsules diabetes mellitus chenodeoxycholic acid nano gel |
| url | https://www.mdpi.com/1999-4923/13/8/1184 |
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