Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats
Background & objective: Diazinon (DZN) as an environmental pollutant adversely effects reproductive system. This study was done to investigate the effect of DZN on sex hormone serum levels and ovarian histological changes in rats. Material & Methods: In this study, 40 female Wistar rats were...
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2017-09-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1158-en.pdf |
| _version_ | 1797679621671485440 |
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| author | Esmaeil Fattahi |
| author_facet | Esmaeil Fattahi |
| author_sort | Esmaeil Fattahi |
| collection | DOAJ |
| description | Background & objective: Diazinon (DZN) as an environmental pollutant adversely effects reproductive system. This study was done to investigate the effect of DZN on sex hormone serum levels and ovarian histological changes in rats.
Material & Methods: In this study, 40 female Wistar rats were randomly divided into four groups including experimental groups 1 and 2 that received 5 and 10 mg/kg diazinon intraperitoneally for 4 weeks respectively. The sham group received olive oil, and the control group received no injection. The animals were anesthetized while proestrus phase and blood samples were collected from the heart and the blood serum sex hormones were measured. by Radioimmunoassay (RIA) method. In addition, ovaries were removed and sections were prepared for histological studies. Data were analyzed using one-way ANOVA and Duncan test.
Results: The results showed that the levels of estrogen and progesterone, number of corpus luteum, primordial, primary, growing, Graafian follicles and diameter of oocyte, corpus luteum, theca and granulosa thickness significantly decreased in the experimental groups compared to control group (p<0.001). Also, the number of atretic follicles in the experimental groups significantly increased compared to that of the control (p<0.001).
Conclusion: The results indicated that diazinon causes changes in ovarian tissue and reduces the estrogen and progesterone hormones and can have adverse effects on oogenesis. |
| first_indexed | 2024-03-11T23:17:27Z |
| format | Article |
| id | doaj.art-580241027a514019b9bd1149a0665c52 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:27Z |
| publishDate | 2017-09-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-580241027a514019b9bd1149a0665c522023-09-20T21:23:04ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232017-09-0172233240Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female RatsEsmaeil Fattahi0 Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Background & objective: Diazinon (DZN) as an environmental pollutant adversely effects reproductive system. This study was done to investigate the effect of DZN on sex hormone serum levels and ovarian histological changes in rats. Material & Methods: In this study, 40 female Wistar rats were randomly divided into four groups including experimental groups 1 and 2 that received 5 and 10 mg/kg diazinon intraperitoneally for 4 weeks respectively. The sham group received olive oil, and the control group received no injection. The animals were anesthetized while proestrus phase and blood samples were collected from the heart and the blood serum sex hormones were measured. by Radioimmunoassay (RIA) method. In addition, ovaries were removed and sections were prepared for histological studies. Data were analyzed using one-way ANOVA and Duncan test. Results: The results showed that the levels of estrogen and progesterone, number of corpus luteum, primordial, primary, growing, Graafian follicles and diameter of oocyte, corpus luteum, theca and granulosa thickness significantly decreased in the experimental groups compared to control group (p<0.001). Also, the number of atretic follicles in the experimental groups significantly increased compared to that of the control (p<0.001). Conclusion: The results indicated that diazinon causes changes in ovarian tissue and reduces the estrogen and progesterone hormones and can have adverse effects on oogenesis.http://jabs.fums.ac.ir/article-1-1158-en.pdfdiazinonovaryoocytegraafian folliclefemale rat |
| spellingShingle | Esmaeil Fattahi Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats Journal of Advanced Biomedical Sciences diazinon ovary oocyte graafian follicle female rat |
| title | Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats |
| title_full | Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats |
| title_fullStr | Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats |
| title_full_unstemmed | Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats |
| title_short | Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats |
| title_sort | investigating the effect of diazinon poison on sex hormone serum levels and ovarian follicle in female rats |
| topic | diazinon ovary oocyte graafian follicle female rat |
| url | http://jabs.fums.ac.ir/article-1-1158-en.pdf |
| work_keys_str_mv | AT esmaeilfattahi investigatingtheeffectofdiazinonpoisononsexhormoneserumlevelsandovarianfollicleinfemalerats |