Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats

Background/Aim: Ulcerative colitis (UC) is a type of chronic inflammatory bowel disease with unknown etiology. Several therapeutic strategies such as consumption of medicinal plants have been used for its treatment. The aim of this study was to evaluate healing effects of Calendula officinalis hydro...

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Main Authors: Nader Tanideh, Akram Jamshidzadeh, Masood Sepehrimanesh, Masood Hosseinzadeh, Omid Koohi-Hosseinabadi, Asma Najibi, Mozhdeh Raam, Sajad Daneshi, Seyedeh-Leili Asadi-Yousefabad
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-01-01
Series:The Saudi Journal of Gastroenterology
Subjects:
Online Access:http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2016;volume=22;issue=1;spage=50;epage=56;aulast=Tanideh
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author Nader Tanideh
Akram Jamshidzadeh
Masood Sepehrimanesh
Masood Hosseinzadeh
Omid Koohi-Hosseinabadi
Asma Najibi
Mozhdeh Raam
Sajad Daneshi
Seyedeh-Leili Asadi-Yousefabad
author_facet Nader Tanideh
Akram Jamshidzadeh
Masood Sepehrimanesh
Masood Hosseinzadeh
Omid Koohi-Hosseinabadi
Asma Najibi
Mozhdeh Raam
Sajad Daneshi
Seyedeh-Leili Asadi-Yousefabad
author_sort Nader Tanideh
collection DOAJ
description Background/Aim: Ulcerative colitis (UC) is a type of chronic inflammatory bowel disease with unknown etiology. Several therapeutic strategies such as consumption of medicinal plants have been used for its treatment. The aim of this study was to evaluate healing effects of Calendula officinalis hydroalcoholic extract in experimentally induced UC in rat. Materials and Methods: Ninety-six rats, weighing 200 ± 20 g, were randomly divided into eight equal groups. UC induced by 3% acetic acid and oral doses of C. officinalis extract, 1500 and 3000 mg/kg, and enema (gel 10% and 20%) were given. Two groups as positive controls were given asacol (enema) and oral mesalamine. Negative control groups were given normal saline and base gel. On days 3 and 7, intestinal histopathology and weight changes, plus oxidative stress indices including malondialdehyde (MDA) level and myeloperoxidase (MPO) activity were assayed. Results: A significant increase in the body weight of rats was seen in the group given C. officinalis extract 3000 mg/kg orally, oral mesalamine, and 20% intracolonic gel form of marigold extract compared with negative control and base gel groups during the experimental period. Acute inflammation and granular atrophy after UC induction were resolved completely completely by both 20% intracolonic gel and 3000 mg/kg orally. An increase in MPO activity and a decrease in MDA level in response to oral and intracolonic gel form of C. officinalis were observed 3 and and 7 days after treatment (P < 0.05). Conclusion: Our results indicate that oral and enema forms of hydroalcoholic extract of C. officinalis can be offered as are potential therapeutic agents for UC induced in rats.
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spelling doaj.art-580406d61d0045a9b68503101b833fa52022-12-21T19:10:09ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492016-01-01221505610.4103/1319-3767.173759Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male ratsNader TanidehAkram JamshidzadehMasood SepehrimaneshMasood HosseinzadehOmid Koohi-HosseinabadiAsma NajibiMozhdeh RaamSajad DaneshiSeyedeh-Leili Asadi-YousefabadBackground/Aim: Ulcerative colitis (UC) is a type of chronic inflammatory bowel disease with unknown etiology. Several therapeutic strategies such as consumption of medicinal plants have been used for its treatment. The aim of this study was to evaluate healing effects of Calendula officinalis hydroalcoholic extract in experimentally induced UC in rat. Materials and Methods: Ninety-six rats, weighing 200 ± 20 g, were randomly divided into eight equal groups. UC induced by 3% acetic acid and oral doses of C. officinalis extract, 1500 and 3000 mg/kg, and enema (gel 10% and 20%) were given. Two groups as positive controls were given asacol (enema) and oral mesalamine. Negative control groups were given normal saline and base gel. On days 3 and 7, intestinal histopathology and weight changes, plus oxidative stress indices including malondialdehyde (MDA) level and myeloperoxidase (MPO) activity were assayed. Results: A significant increase in the body weight of rats was seen in the group given C. officinalis extract 3000 mg/kg orally, oral mesalamine, and 20% intracolonic gel form of marigold extract compared with negative control and base gel groups during the experimental period. Acute inflammation and granular atrophy after UC induction were resolved completely completely by both 20% intracolonic gel and 3000 mg/kg orally. An increase in MPO activity and a decrease in MDA level in response to oral and intracolonic gel form of C. officinalis were observed 3 and and 7 days after treatment (P < 0.05). Conclusion: Our results indicate that oral and enema forms of hydroalcoholic extract of C. officinalis can be offered as are potential therapeutic agents for UC induced in rats.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2016;volume=22;issue=1;spage=50;epage=56;aulast=TanidehHistopathologymarigoldoxidative stressulcerative colitis
spellingShingle Nader Tanideh
Akram Jamshidzadeh
Masood Sepehrimanesh
Masood Hosseinzadeh
Omid Koohi-Hosseinabadi
Asma Najibi
Mozhdeh Raam
Sajad Daneshi
Seyedeh-Leili Asadi-Yousefabad
Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats
The Saudi Journal of Gastroenterology
Histopathology
marigold
oxidative stress
ulcerative colitis
title Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats
title_full Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats
title_fullStr Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats
title_full_unstemmed Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats
title_short Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats
title_sort healing acceleration of acetic acid induced colitis by marigold calendula officinalis in male rats
topic Histopathology
marigold
oxidative stress
ulcerative colitis
url http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2016;volume=22;issue=1;spage=50;epage=56;aulast=Tanideh
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