Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis.
Wood is difficult for most animals to digest due to large amounts of indigestible polymers, but some wood-feeding insects are considered to be able to utilize it as food with the aid of microbial symbionts. Most members of flower longicorn beetles (Coleoptera: Cerambycidae: Lepturinae) feed on necta...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0282351 |
| _version_ | 1797842975319916544 |
|---|---|
| author | Mako Kishigami Fumiaki Matsuoka Akiteru Maeno Shohei Yamagishi Hisashi Abe Wataru Toki |
| author_facet | Mako Kishigami Fumiaki Matsuoka Akiteru Maeno Shohei Yamagishi Hisashi Abe Wataru Toki |
| author_sort | Mako Kishigami |
| collection | DOAJ |
| description | Wood is difficult for most animals to digest due to large amounts of indigestible polymers, but some wood-feeding insects are considered to be able to utilize it as food with the aid of microbial symbionts. Most members of flower longicorn beetles (Coleoptera: Cerambycidae: Lepturinae) feed on nectar and pollen of flowers as adults and wood as larvae. In some lepturines, associations with yeasts are known: female adults possess fungus-storing organs (termed mycetangia) at ovipositors, and larvae also possess such organs (termed mycetomes) in their midguts to carry the associated yeasts. Despite the high diversity of Lepturinae in the world, lepturine-yeast associations, such as the consistency of associated yeasts among the beetle's developmental stages and ecological function of yeast symbionts, have been poorly documented. Here, we investigated the yeast symbiont of the Japanese common lepturine Leptura ochraceofasciata. X-ray computed microtomography revealed that a pair of tube-like, S-shaped mycetangia was located at the basal part of the ovipositor and that a muscle bundle joined the apex of the mycetangium to spiculum ventrale of sternum VIII. All female adults harbored only one yeast species, Scheffersomyces insectosa, in the mycetangia. All larvae harbored S. insectosa exclusively in the mycetomes. Scheffersomyces insectosa was also recovered from surfaces of eggs. Scheffersomyces insectosa assimilated wood-associated sugars including xylose, cellobiose, and xylan in culture. These results suggest the intimate association between L. ochraceofasciata and S. insectosa: S. insectosa is transmitted from the mother to offspring during oviposition and may be related to larval growth in wood. |
| first_indexed | 2024-04-09T16:58:04Z |
| format | Article |
| id | doaj.art-5805162463fd45d68a28b5b918f79c81 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T16:58:04Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5805162463fd45d68a28b5b918f79c812023-04-21T05:34:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01183e028235110.1371/journal.pone.0282351Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis.Mako KishigamiFumiaki MatsuokaAkiteru MaenoShohei YamagishiHisashi AbeWataru TokiWood is difficult for most animals to digest due to large amounts of indigestible polymers, but some wood-feeding insects are considered to be able to utilize it as food with the aid of microbial symbionts. Most members of flower longicorn beetles (Coleoptera: Cerambycidae: Lepturinae) feed on nectar and pollen of flowers as adults and wood as larvae. In some lepturines, associations with yeasts are known: female adults possess fungus-storing organs (termed mycetangia) at ovipositors, and larvae also possess such organs (termed mycetomes) in their midguts to carry the associated yeasts. Despite the high diversity of Lepturinae in the world, lepturine-yeast associations, such as the consistency of associated yeasts among the beetle's developmental stages and ecological function of yeast symbionts, have been poorly documented. Here, we investigated the yeast symbiont of the Japanese common lepturine Leptura ochraceofasciata. X-ray computed microtomography revealed that a pair of tube-like, S-shaped mycetangia was located at the basal part of the ovipositor and that a muscle bundle joined the apex of the mycetangium to spiculum ventrale of sternum VIII. All female adults harbored only one yeast species, Scheffersomyces insectosa, in the mycetangia. All larvae harbored S. insectosa exclusively in the mycetomes. Scheffersomyces insectosa was also recovered from surfaces of eggs. Scheffersomyces insectosa assimilated wood-associated sugars including xylose, cellobiose, and xylan in culture. These results suggest the intimate association between L. ochraceofasciata and S. insectosa: S. insectosa is transmitted from the mother to offspring during oviposition and may be related to larval growth in wood.https://doi.org/10.1371/journal.pone.0282351 |
| spellingShingle | Mako Kishigami Fumiaki Matsuoka Akiteru Maeno Shohei Yamagishi Hisashi Abe Wataru Toki Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis. PLoS ONE |
| title | Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis. |
| title_full | Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis. |
| title_fullStr | Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis. |
| title_full_unstemmed | Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis. |
| title_short | Yeast associated with flower longicorn beetle Leptura ochraceofasciata (Cerambycidae: Lepturinae), with implication for its function in symbiosis. |
| title_sort | yeast associated with flower longicorn beetle leptura ochraceofasciata cerambycidae lepturinae with implication for its function in symbiosis |
| url | https://doi.org/10.1371/journal.pone.0282351 |
| work_keys_str_mv | AT makokishigami yeastassociatedwithflowerlongicornbeetlelepturaochraceofasciatacerambycidaelepturinaewithimplicationforitsfunctioninsymbiosis AT fumiakimatsuoka yeastassociatedwithflowerlongicornbeetlelepturaochraceofasciatacerambycidaelepturinaewithimplicationforitsfunctioninsymbiosis AT akiterumaeno yeastassociatedwithflowerlongicornbeetlelepturaochraceofasciatacerambycidaelepturinaewithimplicationforitsfunctioninsymbiosis AT shoheiyamagishi yeastassociatedwithflowerlongicornbeetlelepturaochraceofasciatacerambycidaelepturinaewithimplicationforitsfunctioninsymbiosis AT hisashiabe yeastassociatedwithflowerlongicornbeetlelepturaochraceofasciatacerambycidaelepturinaewithimplicationforitsfunctioninsymbiosis AT watarutoki yeastassociatedwithflowerlongicornbeetlelepturaochraceofasciatacerambycidaelepturinaewithimplicationforitsfunctioninsymbiosis |