The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2010-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
| _version_ | 1811207280635412480 |
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| author | Geraldo Mäder Priscilla M. Zamberlan Nelson J.R. Fagundes Tielli Magnus Francisco M. Salzano Sandro L. Bonatto Loreta B. Freitas |
| author_facet | Geraldo Mäder Priscilla M. Zamberlan Nelson J.R. Fagundes Tielli Magnus Francisco M. Salzano Sandro L. Bonatto Loreta B. Freitas |
| author_sort | Geraldo Mäder |
| collection | DOAJ |
| description | The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora. |
| first_indexed | 2024-04-12T04:01:41Z |
| format | Article |
| id | doaj.art-580679fc56d2430f819251d15c16fdb3 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-12T04:01:41Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-580679fc56d2430f819251d15c16fdb32022-12-22T03:48:42ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852010-01-0133199108The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)Geraldo MäderPriscilla M. ZamberlanNelson J.R. FagundesTielli MagnusFrancisco M. SalzanoSandro L. BonattoLoreta B. FreitasThe discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018genetic diversityintraspecific variabilityITSPassifloraphylogeography |
| spellingShingle | Geraldo Mäder Priscilla M. Zamberlan Nelson J.R. Fagundes Tielli Magnus Francisco M. Salzano Sandro L. Bonatto Loreta B. Freitas The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) Genetics and Molecular Biology genetic diversity intraspecific variability ITS Passiflora phylogeography |
| title | The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
| title_full | The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
| title_fullStr | The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
| title_full_unstemmed | The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
| title_short | The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
| title_sort | use and limits of its data in the analysis of intraspecific variation in passiflora l passifloraceae |
| topic | genetic diversity intraspecific variability ITS Passiflora phylogeography |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
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