Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection
The work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target analyte....
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MDPI AG
2022-12-01
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Series: | Biosensors |
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Online Access: | https://www.mdpi.com/2079-6374/12/12/1166 |
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author | Nadezhda A. Taranova Alisa A. Bulanaya Anatoly V. Zherdev Boris B. Dzantiev |
author_facet | Nadezhda A. Taranova Alisa A. Bulanaya Anatoly V. Zherdev Boris B. Dzantiev |
author_sort | Nadezhda A. Taranova |
collection | DOAJ |
description | The work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target analyte. Starting from the common ICA protocol with an LOD equal to 11.2 ng/mL, three approaches were realized: (1) replacement of spherical gold nanoparticles with gold nanoflowers having a branched surface (20-fold lowering the LOD); (2) enhanced labeling of immune complexes via nanoparticle aggregates (15-fold lowering); (3) in-situ growth of bound nanoparticles by reduction of gold salts (3-fold lowering). Single and combined implementations of these approaches have been studied. It has been shown that the LOD decrease for combined approaches is close to the multiplied contribution of each of them. The final LOD for FABP was 0.05 ng/mL, which is 220 times lower than the LOD for the common ICA protocol. The efficiency of the enhanced ICA with three combined approaches was confirmed by testing human serum samples for FABP presence and content. The development presents a new efficient technique for rapid sensitive detection of FABP for medical diagnostics. Moreover, the demonstrated multiple enhancements could be applied for various demanded analytes. |
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format | Article |
id | doaj.art-58071dfbe1b24ca1820cfa495b090104 |
institution | Directory Open Access Journal |
issn | 2079-6374 |
language | English |
last_indexed | 2024-03-09T17:16:08Z |
publishDate | 2022-12-01 |
publisher | MDPI AG |
record_format | Article |
series | Biosensors |
spelling | doaj.art-58071dfbe1b24ca1820cfa495b0901042023-11-24T13:37:42ZengMDPI AGBiosensors2079-63742022-12-011212116610.3390/bios12121166Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein DetectionNadezhda A. Taranova0Alisa A. Bulanaya1Anatoly V. Zherdev2Boris B. Dzantiev3A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaThe work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target analyte. Starting from the common ICA protocol with an LOD equal to 11.2 ng/mL, three approaches were realized: (1) replacement of spherical gold nanoparticles with gold nanoflowers having a branched surface (20-fold lowering the LOD); (2) enhanced labeling of immune complexes via nanoparticle aggregates (15-fold lowering); (3) in-situ growth of bound nanoparticles by reduction of gold salts (3-fold lowering). Single and combined implementations of these approaches have been studied. It has been shown that the LOD decrease for combined approaches is close to the multiplied contribution of each of them. The final LOD for FABP was 0.05 ng/mL, which is 220 times lower than the LOD for the common ICA protocol. The efficiency of the enhanced ICA with three combined approaches was confirmed by testing human serum samples for FABP presence and content. The development presents a new efficient technique for rapid sensitive detection of FABP for medical diagnostics. Moreover, the demonstrated multiple enhancements could be applied for various demanded analytes.https://www.mdpi.com/2079-6374/12/12/1166immunochromatographygold nanoparticlessensitivity enhancementfatty acid binding proteincardiomarker |
spellingShingle | Nadezhda A. Taranova Alisa A. Bulanaya Anatoly V. Zherdev Boris B. Dzantiev Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection Biosensors immunochromatography gold nanoparticles sensitivity enhancement fatty acid binding protein cardiomarker |
title | Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection |
title_full | Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection |
title_fullStr | Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection |
title_full_unstemmed | Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection |
title_short | Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection |
title_sort | triple enhancement for sensitive immunochromatographic assay a case study for human fatty acid binding protein detection |
topic | immunochromatography gold nanoparticles sensitivity enhancement fatty acid binding protein cardiomarker |
url | https://www.mdpi.com/2079-6374/12/12/1166 |
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