Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice
<i>Lippia citriodora</i> is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxi...
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/6/875 |
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| author | Katerina Spyridopoulou Tamara Aravidou Evangeli Lampri Eleni Effraimidou Aglaia Pappa Katerina Chlichlia |
| author_facet | Katerina Spyridopoulou Tamara Aravidou Evangeli Lampri Eleni Effraimidou Aglaia Pappa Katerina Chlichlia |
| author_sort | Katerina Spyridopoulou |
| collection | DOAJ |
| description | <i>Lippia citriodora</i> is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxidant potential and to exert antiproliferative activity against breast cancer. The aim of this study was to further investigate potential antitumor effects of <i>L. citriodora</i> oil (LCO) on breast cancer. The in vitro antiproliferative activity of LCO was examined against murine DA3 breast cancer cells by the sulforhodamine B assay. We further explored the LCO’s pro-apoptotic potential with the Annexin-PI method. The LCO’s anti-migratory effect was assessed by the wound-healing assay. LCO was found to inhibit the growth of DA3 cells in vitro, attenuate their migration, and induce apoptosis. Finally, oral administration of LCO for 14 days in mice inhibited by 55% the size of developing tumors in the DA3 murine tumor model. Noteworthy, in the tumor tissue of LCO-treated mice the apoptotic marker cleaved caspase-3 was elevated, while a reduced protein expression of survivin was observed. These results indicate that LCO, as a source of bioactive compounds, has a very interesting nutraceutical potential. |
| first_indexed | 2024-03-10T10:52:34Z |
| format | Article |
| id | doaj.art-5808e68e2dda432c804f132b0708f142 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T10:52:34Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5808e68e2dda432c804f132b0708f1422023-11-21T22:05:55ZengMDPI AGAntioxidants2076-39212021-05-0110687510.3390/antiox10060875Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing MiceKaterina Spyridopoulou0Tamara Aravidou1Evangeli Lampri2Eleni Effraimidou3Aglaia Pappa4Katerina Chlichlia5Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, GreeceDepartment of Medicine, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, Greece<i>Lippia citriodora</i> is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxidant potential and to exert antiproliferative activity against breast cancer. The aim of this study was to further investigate potential antitumor effects of <i>L. citriodora</i> oil (LCO) on breast cancer. The in vitro antiproliferative activity of LCO was examined against murine DA3 breast cancer cells by the sulforhodamine B assay. We further explored the LCO’s pro-apoptotic potential with the Annexin-PI method. The LCO’s anti-migratory effect was assessed by the wound-healing assay. LCO was found to inhibit the growth of DA3 cells in vitro, attenuate their migration, and induce apoptosis. Finally, oral administration of LCO for 14 days in mice inhibited by 55% the size of developing tumors in the DA3 murine tumor model. Noteworthy, in the tumor tissue of LCO-treated mice the apoptotic marker cleaved caspase-3 was elevated, while a reduced protein expression of survivin was observed. These results indicate that LCO, as a source of bioactive compounds, has a very interesting nutraceutical potential.https://www.mdpi.com/2076-3921/10/6/875<i>Lippia citriodora</i>essential oilantiproliferative effectantimigratory activitybreast canceroral administration |
| spellingShingle | Katerina Spyridopoulou Tamara Aravidou Evangeli Lampri Eleni Effraimidou Aglaia Pappa Katerina Chlichlia Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice Antioxidants <i>Lippia citriodora</i> essential oil antiproliferative effect antimigratory activity breast cancer oral administration |
| title | Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice |
| title_full | Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice |
| title_fullStr | Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice |
| title_full_unstemmed | Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice |
| title_short | Antitumor Potential of <i>Lippia citriodora</i> Essential Oil in Breast Tumor-Bearing Mice |
| title_sort | antitumor potential of i lippia citriodora i essential oil in breast tumor bearing mice |
| topic | <i>Lippia citriodora</i> essential oil antiproliferative effect antimigratory activity breast cancer oral administration |
| url | https://www.mdpi.com/2076-3921/10/6/875 |
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