Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i>
Paralytic shellfish toxins (PSTs) are an increasingly important source of pollution. Bivalves, as the main transmission medium, accumulate and metabolize PSTs while protecting themselves from damage. At present, the resistance mechanism of bivalves to PSTs is unclear. In this study, <i>Mytilus...
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MDPI AG
2022-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/8/516 |
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| author | Chenfan Dong Haiyan Wu Guanchao Zheng Jixing Peng Mengmeng Guo Zhijun Tan |
| author_facet | Chenfan Dong Haiyan Wu Guanchao Zheng Jixing Peng Mengmeng Guo Zhijun Tan |
| author_sort | Chenfan Dong |
| collection | DOAJ |
| description | Paralytic shellfish toxins (PSTs) are an increasingly important source of pollution. Bivalves, as the main transmission medium, accumulate and metabolize PSTs while protecting themselves from damage. At present, the resistance mechanism of bivalves to PSTs is unclear. In this study, <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> were used as experimental shellfish species for in situ monitoring. We compared the inflammatory-related gene responses of the two shellfish during PSTs exposure by using transcriptomes. The results showed that the accumulation and metabolism rate of PSTs in <i>M. galloprovincialis</i> was five-fold higher than that in <i>A. irradians</i>. The inflammatory balance mechanism of <i>M. galloprovincialis</i> involved the co-regulation of the MAPK-based and AMPK-based anti-inflammatory pathways. <i>A. irradians</i> bore a higher risk of death because it did not have the balance system, and the regulation of apoptosis-related pathways such as the PI3K-AKT signaling pathway were upregulated. Taken together, the regulation of the inflammatory balance coincides with the ability of bivalves to cope with PSTs. Inflammation is an important factor that affects the metabolic pattern of PSTs in bivalves. This study provides new evidence to support the studies on the resistance mechanism of bivalves to PSTs. |
| first_indexed | 2024-03-09T09:47:46Z |
| format | Article |
| id | doaj.art-5809c757b75247cebcce4a5190f6806a |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T09:47:46Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | Toxins |
| spelling | doaj.art-5809c757b75247cebcce4a5190f6806a2023-12-02T00:23:18ZengMDPI AGToxins2072-66512022-07-0114851610.3390/toxins14080516Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i>Chenfan Dong0Haiyan Wu1Guanchao Zheng2Jixing Peng3Mengmeng Guo4Zhijun Tan5Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaParalytic shellfish toxins (PSTs) are an increasingly important source of pollution. Bivalves, as the main transmission medium, accumulate and metabolize PSTs while protecting themselves from damage. At present, the resistance mechanism of bivalves to PSTs is unclear. In this study, <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> were used as experimental shellfish species for in situ monitoring. We compared the inflammatory-related gene responses of the two shellfish during PSTs exposure by using transcriptomes. The results showed that the accumulation and metabolism rate of PSTs in <i>M. galloprovincialis</i> was five-fold higher than that in <i>A. irradians</i>. The inflammatory balance mechanism of <i>M. galloprovincialis</i> involved the co-regulation of the MAPK-based and AMPK-based anti-inflammatory pathways. <i>A. irradians</i> bore a higher risk of death because it did not have the balance system, and the regulation of apoptosis-related pathways such as the PI3K-AKT signaling pathway were upregulated. Taken together, the regulation of the inflammatory balance coincides with the ability of bivalves to cope with PSTs. Inflammation is an important factor that affects the metabolic pattern of PSTs in bivalves. This study provides new evidence to support the studies on the resistance mechanism of bivalves to PSTs.https://www.mdpi.com/2072-6651/14/8/516paralytic shellfish toxinoxidative stressinflammatory balance<i>Mytilus galloprovincialis</i><i>Argopecten irradians</i> |
| spellingShingle | Chenfan Dong Haiyan Wu Guanchao Zheng Jixing Peng Mengmeng Guo Zhijun Tan Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> Toxins paralytic shellfish toxin oxidative stress inflammatory balance <i>Mytilus galloprovincialis</i> <i>Argopecten irradians</i> |
| title | Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> |
| title_full | Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> |
| title_fullStr | Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> |
| title_full_unstemmed | Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> |
| title_short | Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in <i>Mytilus galloprovincialis</i> and <i>Argopecten irradians</i> |
| title_sort | transcriptome analysis reveals mapk ampk as a key regulator of the inflammatory response in pst detoxification in i mytilus galloprovincialis i and i argopecten irradians i |
| topic | paralytic shellfish toxin oxidative stress inflammatory balance <i>Mytilus galloprovincialis</i> <i>Argopecten irradians</i> |
| url | https://www.mdpi.com/2072-6651/14/8/516 |
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