Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note
Introduction: Nuclear medicine patients are isolated in a room after the injection of a radiopharmaceutical. They may be active Wi-Fi option of its smartphone mobile or other environmental radiofrequency waves. The hypothesis of this study was the evaluation of increased biological effects of the si...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580822000334 |
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| author | Ehsan Khodamoradi Shima Afrashi Karim Khoshgard Farshid Fathi Soodeh Shahasavari Rasool Azmoonfar Masoud Najafi |
| author_facet | Ehsan Khodamoradi Shima Afrashi Karim Khoshgard Farshid Fathi Soodeh Shahasavari Rasool Azmoonfar Masoud Najafi |
| author_sort | Ehsan Khodamoradi |
| collection | DOAJ |
| description | Introduction: Nuclear medicine patients are isolated in a room after the injection of a radiopharmaceutical. They may be active Wi-Fi option of its smartphone mobile or other environmental radiofrequency waves. The hypothesis of this study was the evaluation of increased biological effects of the simultaneous exposure to gamma-ray and the Wi-Fi waves by measuring the level of the increased double strand-breaks DNA in peripheral blood lymphocyte in the rat. Materials and methods: Fifty male Wistar rats were exposed for 2, 24, and 72 h only by Wi-Fi, 99m Tc, and simultaneously by Wi-Fi and 99m Tc. The power density levels of Wi-Fi emitter at 15 cm was 4.2nW/ cm2. An activity of 100 μCi of 99m Tc was injected intraperitoneally. Blood samples were taken by cardiac puncture following general anesthesia. Mononuclear cells are extraction by Ficoll-Hypaque density gradient centrifugation. The number of gamma-H2AX foci per nucleus was counted by flow cytometry. The statistical differences between experimental groups at 2, 24, and 72 h were determined with a repeated measure's analysis of variance. The significant difference between groups at the same time was analyzed with the Kruskal-Wallis Test. Results: The manner of gamma-H2AX expression was not the same for three groups in time. The number of gamma-H2AX foci between the three groups was a significant difference after 72 h. Conclusion: Simultaneous Wi-Fi and gamma-ray exposures can increase the number of double-strand break DNA in peripheral blood lymphocytes to exposure of gamma-ray to 72 h after technetium injection in the rat. |
| first_indexed | 2024-04-12T11:02:20Z |
| format | Article |
| id | doaj.art-5814f13527844bbd811d546f79c10c73 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-12T11:02:20Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-5814f13527844bbd811d546f79c10c732022-12-22T03:35:54ZengElsevierBiochemistry and Biophysics Reports2405-58082022-07-0130101232Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection noteEhsan Khodamoradi0Shima Afrashi1Karim Khoshgard2Farshid Fathi3Soodeh Shahasavari4Rasool Azmoonfar5Masoud Najafi6Department of Radiology and Nuclear Medicine, School of Paramedical Sciences, Kermanshah University of Medical Sciences, Kermanshah, IranStudent Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Medical Physics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Corresponding author. Medical Physics Department, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Health Information Management, School of Paramedical, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Radiology, School of Paramedical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran; Corresponding author. Department of Radiology, School of Paramedical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.Department of Radiology and Nuclear Medicine, School of Paramedical Sciences, Kermanshah University of Medical Sciences, Kermanshah, IranIntroduction: Nuclear medicine patients are isolated in a room after the injection of a radiopharmaceutical. They may be active Wi-Fi option of its smartphone mobile or other environmental radiofrequency waves. The hypothesis of this study was the evaluation of increased biological effects of the simultaneous exposure to gamma-ray and the Wi-Fi waves by measuring the level of the increased double strand-breaks DNA in peripheral blood lymphocyte in the rat. Materials and methods: Fifty male Wistar rats were exposed for 2, 24, and 72 h only by Wi-Fi, 99m Tc, and simultaneously by Wi-Fi and 99m Tc. The power density levels of Wi-Fi emitter at 15 cm was 4.2nW/ cm2. An activity of 100 μCi of 99m Tc was injected intraperitoneally. Blood samples were taken by cardiac puncture following general anesthesia. Mononuclear cells are extraction by Ficoll-Hypaque density gradient centrifugation. The number of gamma-H2AX foci per nucleus was counted by flow cytometry. The statistical differences between experimental groups at 2, 24, and 72 h were determined with a repeated measure's analysis of variance. The significant difference between groups at the same time was analyzed with the Kruskal-Wallis Test. Results: The manner of gamma-H2AX expression was not the same for three groups in time. The number of gamma-H2AX foci between the three groups was a significant difference after 72 h. Conclusion: Simultaneous Wi-Fi and gamma-ray exposures can increase the number of double-strand break DNA in peripheral blood lymphocytes to exposure of gamma-ray to 72 h after technetium injection in the rat.http://www.sciencedirect.com/science/article/pii/S2405580822000334Wi-Fi99m TcDouble strand-break DNAGamma-H2AXFlowcytometry |
| spellingShingle | Ehsan Khodamoradi Shima Afrashi Karim Khoshgard Farshid Fathi Soodeh Shahasavari Rasool Azmoonfar Masoud Najafi Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note Biochemistry and Biophysics Reports Wi-Fi 99m Tc Double strand-break DNA Gamma-H2AX Flowcytometry |
| title | Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note |
| title_full | Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note |
| title_fullStr | Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note |
| title_full_unstemmed | Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note |
| title_short | Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note |
| title_sort | simultaneous effect of gamma and wi fi radiation on gamma h2ax expression in peripheral blood of rat a radio protection note |
| topic | Wi-Fi 99m Tc Double strand-break DNA Gamma-H2AX Flowcytometry |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822000334 |
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