Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust
Thinopyrum intermedium (JJJsJsStSt, 2n = 6x = 42), a member of tertiary gene pool of hexaploid wheat (Triticum aestivum L., AABBDD, 2n = 6x = 42), provides several beneficial genes for wheat improvement. In this study, line CH51 was developed from the BC1F8 progeny of a partial wheat-Th. intermedium...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006281/full |
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| author | Xiaojun Zhang Jianbo Li Jianbo Li Jianbo Li Yudi Ge Haixia Guan Guangrong Li Shuwei Zhang Xiaolu Wang Xin Li Zhijian Chang Peng Zhang Juqing Jia Cheng Liu |
| author_facet | Xiaojun Zhang Jianbo Li Jianbo Li Jianbo Li Yudi Ge Haixia Guan Guangrong Li Shuwei Zhang Xiaolu Wang Xin Li Zhijian Chang Peng Zhang Juqing Jia Cheng Liu |
| author_sort | Xiaojun Zhang |
| collection | DOAJ |
| description | Thinopyrum intermedium (JJJsJsStSt, 2n = 6x = 42), a member of tertiary gene pool of hexaploid wheat (Triticum aestivum L., AABBDD, 2n = 6x = 42), provides several beneficial genes for wheat improvement. In this study, line CH51 was developed from the BC1F8 progeny of a partial wheat-Th. intermedium amphiploid TAI8335 (2n = 56) and wheat cultivar (cv.) Jintai 170. Somatic metaphase chromosome counting showed that CH51 had stable 42 chromosomes. Genomic in situ hybridization (GISH) analysis showed that CH51 had 40 wheat chromosomes and two Th. intermedium chromosomes involving translocation between Js- and St-genome chromosomes. Non-denaturing fluorescence in situ hybridization (ND-FISH) analysis revealed that CH51 lacked a pair of wheat chromosome 6B. Wheat 55K SNP array analysis verified that chromosome 6B had the highest percentage of missing SNP loci in both CH51 and Chinese Spring (CS) nullisomic 6B-tetrasomic 6D (CS-N6BT6D) and had the highest percentage of polymorphic SNP loci between CH51 and cv. Jintai 170. We identified that CH51 was a wheat-Th. intermedium T6StS.6JsL (6B) disomic substitution line. Disease resistance assessment showed that CH51 exhibited high levels of resistance to the prevalent Chinese leaf rust and stripe rust races in the field. Therefore, the newly developed line CH51 can be utilized as a potential germplasm in wheat disease resistance breeding. |
| first_indexed | 2024-12-10T15:03:10Z |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T15:03:10Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-581fddb254fe414b882af1eb40c87a742022-12-22T01:44:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10062811006281Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rustXiaojun Zhang0Jianbo Li1Jianbo Li2Jianbo Li3Yudi Ge4Haixia Guan5Guangrong Li6Shuwei Zhang7Xiaolu Wang8Xin Li9Zhijian Chang10Peng Zhang11Juqing Jia12Cheng Liu13College of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaPlant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, AustraliaSchool of Life Sciences, Shanxi University, Taiyuan, Shanxi, ChinaPlant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, AustraliaSchool of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaCollege of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaCollege of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaPlant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, AustraliaCollege of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaThinopyrum intermedium (JJJsJsStSt, 2n = 6x = 42), a member of tertiary gene pool of hexaploid wheat (Triticum aestivum L., AABBDD, 2n = 6x = 42), provides several beneficial genes for wheat improvement. In this study, line CH51 was developed from the BC1F8 progeny of a partial wheat-Th. intermedium amphiploid TAI8335 (2n = 56) and wheat cultivar (cv.) Jintai 170. Somatic metaphase chromosome counting showed that CH51 had stable 42 chromosomes. Genomic in situ hybridization (GISH) analysis showed that CH51 had 40 wheat chromosomes and two Th. intermedium chromosomes involving translocation between Js- and St-genome chromosomes. Non-denaturing fluorescence in situ hybridization (ND-FISH) analysis revealed that CH51 lacked a pair of wheat chromosome 6B. Wheat 55K SNP array analysis verified that chromosome 6B had the highest percentage of missing SNP loci in both CH51 and Chinese Spring (CS) nullisomic 6B-tetrasomic 6D (CS-N6BT6D) and had the highest percentage of polymorphic SNP loci between CH51 and cv. Jintai 170. We identified that CH51 was a wheat-Th. intermedium T6StS.6JsL (6B) disomic substitution line. Disease resistance assessment showed that CH51 exhibited high levels of resistance to the prevalent Chinese leaf rust and stripe rust races in the field. Therefore, the newly developed line CH51 can be utilized as a potential germplasm in wheat disease resistance breeding.https://www.frontiersin.org/articles/10.3389/fpls.2022.1006281/fullThinopyrum intermediumGISHFISHwheat 55K SNP arraydisease resistance |
| spellingShingle | Xiaojun Zhang Jianbo Li Jianbo Li Jianbo Li Yudi Ge Haixia Guan Guangrong Li Shuwei Zhang Xiaolu Wang Xin Li Zhijian Chang Peng Zhang Juqing Jia Cheng Liu Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust Frontiers in Plant Science Thinopyrum intermedium GISH FISH wheat 55K SNP array disease resistance |
| title | Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust |
| title_full | Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust |
| title_fullStr | Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust |
| title_full_unstemmed | Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust |
| title_short | Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust |
| title_sort | molecular cytogenetic characterization of a new wheat thinopyrum intermedium homoeologous group 6 chromosome disomic substitution line with resistance to leaf rust and stripe rust |
| topic | Thinopyrum intermedium GISH FISH wheat 55K SNP array disease resistance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006281/full |
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