Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma
Abstract Background Tropomyosin 4 (TPM4), a member of the tropomyosin family, is aberrantly expressed and plays an important role in a variety of cancers. However, studies on TPM4 in glioma patients are currently lacking. Objective Our study aimed to evaluate the diagnostic and prognostic characteri...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Neurology |
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| Online Access: | https://doi.org/10.1186/s12883-023-03058-0 |
| _version_ | 1828063687006486528 |
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| author | Yao Li Yanan Zhang Zeyu Wu Peng Sun |
| author_facet | Yao Li Yanan Zhang Zeyu Wu Peng Sun |
| author_sort | Yao Li |
| collection | DOAJ |
| description | Abstract Background Tropomyosin 4 (TPM4), a member of the tropomyosin family, is aberrantly expressed and plays an important role in a variety of cancers. However, studies on TPM4 in glioma patients are currently lacking. Objective Our study aimed to evaluate the diagnostic and prognostic characteristics of TPM4 in glioma and its correlation with immune infiltration. Methods Bioinformatic analysis was performed to determine whether TPM4 has diagnostic and prognostic value for glioma. The following databases and analytical tools were used to explore the clinical significance of TPM4 in glioma: TCGA, GTEx, GEO, STRING, and TISIDB. Results Our study showed that the mRNA and protein expression levels of TPM4 were significantly higher in glioma than in healthy brain tissue. Kaplan–Meier analysis indicated that high expression of TPM4 in glioma correlated with poor prognosis. Univariate Cox analysis indicated that the high expression level of TPM4 in glioma was an independent prognostic characteristic for low overall survival (OS). The areas under the 1-year survival ROC, 2-year survival ROC, and 3-year survival ROC were all greater than 0.8. GO and KEGG enrichment analysis and GSEA showed that humoral immune response and cytokine receptor interaction were significantly enriched in the TPM4 high expression group, where M phase of the cell cycle, neutrophil degranulation, signaling by interleukins, and signaling by rho GTPases were significantly enriched. Furthermore, according to the analysis of immune cell infiltration, TPM4 was associated with tumor infiltration of a variety of immune cells. Conclusions In conclusion, our study suggests that TPM4 may be an effective prognostic biomarker for glioma patients, providing new ideas and research directions for glioma research. |
| first_indexed | 2024-04-10T22:47:00Z |
| format | Article |
| id | doaj.art-5823d8ca4ee54a27a0c48c2d20885a34 |
| institution | Directory Open Access Journal |
| issn | 1471-2377 |
| language | English |
| last_indexed | 2024-04-10T22:47:00Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj.art-5823d8ca4ee54a27a0c48c2d20885a342023-01-15T12:15:03ZengBMCBMC Neurology1471-23772023-01-0123111610.1186/s12883-023-03058-0Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with gliomaYao Li0Yanan Zhang1Zeyu Wu2Peng Sun3Department of Neurosurgery, Qingdao UniversityDepartment of Anesthesiology, Weifang Medical UniversityDepartment of Neurosurgery, Affiliated Hospital of Qingdao UniversityDepartment of Neurosurgery, Affiliated Hospital of Qingdao UniversityAbstract Background Tropomyosin 4 (TPM4), a member of the tropomyosin family, is aberrantly expressed and plays an important role in a variety of cancers. However, studies on TPM4 in glioma patients are currently lacking. Objective Our study aimed to evaluate the diagnostic and prognostic characteristics of TPM4 in glioma and its correlation with immune infiltration. Methods Bioinformatic analysis was performed to determine whether TPM4 has diagnostic and prognostic value for glioma. The following databases and analytical tools were used to explore the clinical significance of TPM4 in glioma: TCGA, GTEx, GEO, STRING, and TISIDB. Results Our study showed that the mRNA and protein expression levels of TPM4 were significantly higher in glioma than in healthy brain tissue. Kaplan–Meier analysis indicated that high expression of TPM4 in glioma correlated with poor prognosis. Univariate Cox analysis indicated that the high expression level of TPM4 in glioma was an independent prognostic characteristic for low overall survival (OS). The areas under the 1-year survival ROC, 2-year survival ROC, and 3-year survival ROC were all greater than 0.8. GO and KEGG enrichment analysis and GSEA showed that humoral immune response and cytokine receptor interaction were significantly enriched in the TPM4 high expression group, where M phase of the cell cycle, neutrophil degranulation, signaling by interleukins, and signaling by rho GTPases were significantly enriched. Furthermore, according to the analysis of immune cell infiltration, TPM4 was associated with tumor infiltration of a variety of immune cells. Conclusions In conclusion, our study suggests that TPM4 may be an effective prognostic biomarker for glioma patients, providing new ideas and research directions for glioma research.https://doi.org/10.1186/s12883-023-03058-0TPM4GliomaOverall survivalPrognosisImmune infiltration |
| spellingShingle | Yao Li Yanan Zhang Zeyu Wu Peng Sun Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma BMC Neurology TPM4 Glioma Overall survival Prognosis Immune infiltration |
| title | Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma |
| title_full | Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma |
| title_fullStr | Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma |
| title_full_unstemmed | Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma |
| title_short | Overexpression of TPM4 is associated with worse prognosis and immune infiltration in patients with glioma |
| title_sort | overexpression of tpm4 is associated with worse prognosis and immune infiltration in patients with glioma |
| topic | TPM4 Glioma Overall survival Prognosis Immune infiltration |
| url | https://doi.org/10.1186/s12883-023-03058-0 |
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