Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model
Background and aim: The fruits of Cocos nucifera have long been used in traditional medicine as anti-inflammatory, antimicrobial, antiparasitic, anticancer, and cardiotonic. This study aimed to evaluate the anti-diabetic and antilipidemic activities in the diabetic rat model.Material and methods: Co...
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| Format: | Article |
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International Journal of Scientific Research in Dental and Medical Sciences (IJSRDMS)
2022-03-01
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| Series: | International Journal of Scientific Research in Dental and Medical Sciences |
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| Online Access: | http://www.ijsrdms.com/article_144804_d668960f6b0f8b90431a4f45b30f345c.pdf |
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| author | Farjana Akter N. M. Mahmudul Bhuiya Nazmul Hasan Begum Rokeya Sheikh Raihan Zobaer Al Mahmud |
| author_facet | Farjana Akter N. M. Mahmudul Bhuiya Nazmul Hasan Begum Rokeya Sheikh Raihan Zobaer Al Mahmud |
| author_sort | Farjana Akter |
| collection | DOAJ |
| description | Background and aim: The fruits of Cocos nucifera have long been used in traditional medicine as anti-inflammatory, antimicrobial, antiparasitic, anticancer, and cardiotonic. This study aimed to evaluate the anti-diabetic and antilipidemic activities in the diabetic rat model.Material and methods: Cocos nucifera endocarp was extracted with Methanol and then fractionated with different solvents, namely h-hexane, Chloroform, and ethyl acetate. A total of 30 Streptozotocin-induced diabetic rats were used in six experimental groups. All the animals were treated for 14 days period. The measured outcomes were fasting blood sugar (FBS), lipid profiles, acute hypoglycaemic effects, and changes in body weights. Antioxidative studies and phytochemical screening were also performed.Results: A significant hypoglycemic effect was found in the group treated with ethyl acetate fractions (6.33 ± 0.32 mmol/L, p<0.05) when compared to placebo (7.85±0.38 mmol/L). After a glucose load, the same fraction also showed a significant antihyperglycemic effect within 75 minutes of treatment. Both ethyl acetate and chloroform fractions reduced the baseline serum cholesterol and triglyceride levels. However, no significant change in body weight was reported in any treatment groups. In the DPPH free radical scavenging assay, the methanolic fraction and ethyl acetate fractions showed notable free radical scavenging activity with IC50 value 32.31 μg/ml and 27.55 respectively, compared to standard Tert-Butyl-1-hydroxytolune (23.92 μg/ml), indicating potential antioxidative properties.Conclusions: Cocos nucifera endocarp showed promising anti-diabetic and antilipidemic properties. The ethyl acetate soluble fraction might be further explored to isolate active medicinal compounds in drug discovery. |
| first_indexed | 2024-12-13T14:17:03Z |
| format | Article |
| id | doaj.art-5823fd732544419fb851a7665d48cb97 |
| institution | Directory Open Access Journal |
| issn | 2676-5497 2676-5373 |
| language | English |
| last_indexed | 2024-12-13T14:17:03Z |
| publishDate | 2022-03-01 |
| publisher | International Journal of Scientific Research in Dental and Medical Sciences (IJSRDMS) |
| record_format | Article |
| series | International Journal of Scientific Research in Dental and Medical Sciences |
| spelling | doaj.art-5823fd732544419fb851a7665d48cb972022-12-21T23:42:16ZengInternational Journal of Scientific Research in Dental and Medical Sciences (IJSRDMS)International Journal of Scientific Research in Dental and Medical Sciences2676-54972676-53732022-03-014181510.30485/ijsrdms.2022.320342.1228144804Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat ModelFarjana Akter0N. M. Mahmudul Bhuiya1Nazmul Hasan2Begum Rokeya3Sheikh Raihan4Zobaer Al Mahmud5Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, BangladeshDepartment of Pharmacy, Jagannath University, Dhaka, BangladeshDepartment of Pharmacy, Chittagong University, Chittagong, BangladeshDepartment of Pharmacology, Bangladesh University of Health Sciences, Dhaka, BangladeshDepartment of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, BangladeshDepartment of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, BangladeshBackground and aim: The fruits of Cocos nucifera have long been used in traditional medicine as anti-inflammatory, antimicrobial, antiparasitic, anticancer, and cardiotonic. This study aimed to evaluate the anti-diabetic and antilipidemic activities in the diabetic rat model.Material and methods: Cocos nucifera endocarp was extracted with Methanol and then fractionated with different solvents, namely h-hexane, Chloroform, and ethyl acetate. A total of 30 Streptozotocin-induced diabetic rats were used in six experimental groups. All the animals were treated for 14 days period. The measured outcomes were fasting blood sugar (FBS), lipid profiles, acute hypoglycaemic effects, and changes in body weights. Antioxidative studies and phytochemical screening were also performed.Results: A significant hypoglycemic effect was found in the group treated with ethyl acetate fractions (6.33 ± 0.32 mmol/L, p<0.05) when compared to placebo (7.85±0.38 mmol/L). After a glucose load, the same fraction also showed a significant antihyperglycemic effect within 75 minutes of treatment. Both ethyl acetate and chloroform fractions reduced the baseline serum cholesterol and triglyceride levels. However, no significant change in body weight was reported in any treatment groups. In the DPPH free radical scavenging assay, the methanolic fraction and ethyl acetate fractions showed notable free radical scavenging activity with IC50 value 32.31 μg/ml and 27.55 respectively, compared to standard Tert-Butyl-1-hydroxytolune (23.92 μg/ml), indicating potential antioxidative properties.Conclusions: Cocos nucifera endocarp showed promising anti-diabetic and antilipidemic properties. The ethyl acetate soluble fraction might be further explored to isolate active medicinal compounds in drug discovery.http://www.ijsrdms.com/article_144804_d668960f6b0f8b90431a4f45b30f345c.pdfcocoshypoglycemic agentshypolipidemic agentsoxidative stress |
| spellingShingle | Farjana Akter N. M. Mahmudul Bhuiya Nazmul Hasan Begum Rokeya Sheikh Raihan Zobaer Al Mahmud Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model International Journal of Scientific Research in Dental and Medical Sciences cocos hypoglycemic agents hypolipidemic agents oxidative stress |
| title | Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model |
| title_full | Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model |
| title_fullStr | Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model |
| title_full_unstemmed | Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model |
| title_short | Cocos Nucifera Endocarp Extract Exhibits Anti-diabetic and Antilipidemic Activities in Diabetic Rat Model |
| title_sort | cocos nucifera endocarp extract exhibits anti diabetic and antilipidemic activities in diabetic rat model |
| topic | cocos hypoglycemic agents hypolipidemic agents oxidative stress |
| url | http://www.ijsrdms.com/article_144804_d668960f6b0f8b90431a4f45b30f345c.pdf |
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